4
WRITE
PROTECTION
(Locked/-
Unlocked)
"
locked/unlocked"
}Prevents program from being edited when in Locked
mode.
PRESSURE
SETTING
(Range 0-999)
Reports the programmed value of the air pressure during the active
cooling phase of the pull cycle.
DATE/TIME
Reports the date and time of the last program edit.
HEAT
(Range 0-999)
HEAT controls the level of electrical current supplied to the filament. The
HEAT required to melt a piece of glass is a function of the filament
installed and the particular glass size and composition. It is important
that the HEAT value be set relative to the Ramp Test value as discussed
in the Operation Section. Generally, changes to HEAT will be made in
steps of about 5 units since in most cases smaller changes will have little
effect.
PULL
(Range 0-255)
This parameter controls the force of the hard pull. In general, the higher
the PULL, the smaller the pipette’s tip diameter and the longer the taper.
Useful changes in PULL strength are 10 units or more to see an effect.
VELOCITY
(Range 0-255)
The velocity of the glass carriage system is measured as the glass softens
and begins to pull apart under a constant load. The increasing velocity of
the initial pull is determined by the viscosity of the glass, which in turn is
a function of the glass temperature. The adjustable velocity allows for a
selection of a precise glass temperature as the trip point for the hard pull.
Useful values for velocity range from 10 to 150 with lower values being
used for patch and injection pipettes and higher values for micropipettes.
SEE THE PROGRAMS SECTION FOR A DISCUSSION OF THE
SIGNIFICANCE OF VELOCITY=0.
TIME
(Range 0-255)
TIME is one of two available modes of cooling and controls the length of
time the cooling air is active. If VEL>0 then one unit of TIME represents
1/2ms. If VEL=0 then one unit of TIME represents 10ms. SEE THE
PROGRAMS SECTION FOR A DISCUSSION OF THE SIGNIFICANCE
OF TIME=0.
DELAY
(Range 0-255)
DELAY is a cooling mode, which controls the delay time between when
the heat turns off and when the hard pull is activated. The air is
automatically turned on for 300ms. The higher the DELAY value, the
cooler the glass will be when the hard pull is executed. Thus, increasing
the DELAY results with decreased taper length and increased tip
diameter. If VEL>0 then one unit of DELAY represents 1 (one)
millisecond (ms). If VEL=0 (when using fire-polish mode), then one unit
represents 10 (ten) ms. SEE THE PROGRAMS SECTION FOR A
DISCUSSION OF THE SIGNIFICANCE OF DELAY=0.
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)
Summary of Contents for P-97
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