Sutter Instrument BV-10 Operation Manual Download Page 1

Page: 1 / 38

One Digital Drive

Novato, CA 94949

Voice: 415-883-0128 Web: www.sutter.com

Fax:

415-883-0572 Email: [email protected]

BV-10

Micropipette Beveler

Operation Manual

Rev. 3.00 ( 20180423)

Summary of Contents for BV-10

Page 1: ...One Digital Drive Novato CA 94949 Voice 415 883 0128 Web www sutter com Fax 415 883 0572 Email info sutter com BV 10 Micropipette Beveler Operation Manual Rev 3 00 20180423...

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Page 5: ...n for which it is designed SAFETY WARNINGS AND PRECAUTIONS Electrical Operate the BV 10 using 110 120 V AC 60 Hz or 220 240 V AC 50 Hz line voltage This instrument is designed for connection to a stan...

Page 6: ...this device Operate this unit using the indicated line voltage This unit is designed for operation in a laboratory environment Pollution Degree I This unit is designed for connection to a standard lab...

Page 7: ...ion of Package Contents 13 2 2 Set Up and Assembly Instructions 14 3 OPERATION 17 3 1 Grinding Procedure 17 3 1 1 General Information 17 3 1 2 Control of Beveling by Measuring Electrical Resistance 18...

Page 8: ...1 1 Manipulator 9 Figure 1 2 Pedestal Grinding Assembly 10 Figure 1 3 BVM CE Electrode Resistance Meter 11 Figure 2 1 BV 10 beveler component layout 13 Figure 2 2 Pedestal grinding plate assembly 15 F...

Page 9: ...ate rotates on a stationary optically flat pedestal by virtue of a magnetic coupling with a rotating drive ring Rotational speed is approximately 60 rpm Pipette positioning relative to the grinding pl...

Page 10: ...Electrode Impedance Meter and BV 10S 40X Stereo Microscope 1 4 Technical Support Unlimited technical support is provided by Sutter Instrument Company at no charge to our customers Our technical suppor...

Page 11: ...ontrol knob used to position the pipette just above the grinding plate surface a fine control knob micrometer used to control the actual beveling of pipettes The sensitivity of the fine control can be...

Page 12: ...quartz surface a magnetic drive ring that rotates around the pedestal and upper and lower retaining rings which hold the grinding plate The grinding plate rotates wobble free on the flat quartz surfa...

Page 13: ...ler Resistance is indicated by a precision analog meter which allows the user to easily identify changes in microelectrode resistance while beveling Each meter is calibrated using the active lead that...

Page 14: ...To aid viewing the beveling process an optical attachment is available The unit consists of a 40x stereomicroscope and mounting hardware For large diameter tips 1 m the attachment allows the user to b...

Page 15: ...x 1 1 Beveler motor base with motor housing halogen lamp and pedestal assembly 2 Reference wick 3 Reference wick holder 4 Pedestal oil 5 Degreaser 6 Operating Manual 7 Abrasive grinding plates 2 and r...

Page 16: ...05 to 1 m and a 0 3 m alumina abrasive plate for beveling pipette tips in the 0 5 to 1 m range Diamond coated plates are available for beveling larger tips and can be ordered from Sutter Instrument Co...

Page 17: ...hole in the mounting block The end of the bar with the threaded hole and flat face is directed to the left The end of the bar with the setscrew is directed to the right Orient the bar such that the f...

Page 18: ...te the scope counter clockwise relative to the vertical post To adjust the angle of the scope relative to the beveler plate hold the scope with one hand while loosening the thumbscrew that clamps the...

Page 19: ...e abrasive surface and then monitoring the progress of beveling until a predetermined criterion is met In cases where the tips are so fine that they cannot be viewed with a microscope monitoring the m...

Page 20: ...er The electrode will first make contact with the saline ring and then with the grinding plate As soon as the first change in resistance is noted stop advancing the electrode If the resistance remains...

Page 21: ...s from the tip Now continue to lower the tip until the character of the bubble stream changes usually it appears that the bubble stream has disappeared though it has probably broken up into a stream o...

Page 22: ...ement from mechanical shocks When you wish to make a reading switch to the X5 range If the reading is less than 100 Mohms switch to the X0 1 range This procedure will give the greatest resolution When...

Page 23: ...single number that uniquely defines the resistance of a pipette Thus two accurate instruments employing different measuring currents could produce completely different measured values of an electrode...

Page 24: ...BV 10 OPERATION MANUAL REV 3 00 20180423 22 This page intentionally left blank...

Page 25: ...with a Kimwipe1 Rinse the plate with distilled water and blot dry with a Kimwipe It is necessary to have the abrasive surface cleaned of grease and oil so that a thin layer of saline or water may be...

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Page 27: ...sive plate for scoring If either surface is scored call Sutter Instrument for technical support 5 3 Beveling Takes a Long Time Abrasive rating of plate is too fine for desired tip size Try a larger ab...

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Page 29: ...at the factory The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect after which the cost is the responsibility of the customer The...

Page 30: ...BV 10 OPERATION MANUAL REV 3 00 20180423 28 This page intentionally left blank...

Page 31: ...0 to 20 m tip sizes 104E Diamond abrasive plate very fine 0 5 m light brown for 0 7 to 2 0 m tip sizes 104F Diamond abrasive plate extra fine 0 1 m green for 0 2 to 1 0 m tip sizes 007 Degreaser bott...

Page 32: ...BV 10 OPERATION MANUAL REV 3 00 20180423 30 This page intentionally left blank...

Page 33: ...wer entry module Figure C 1 Power entry module The fuse that is readily visible in the fuse holder when you take it out is the one that is active when the holder is installed A spare fuse is also stor...

Page 34: ...ace only with the same type and rating Miniature Fuse 5 x 20 mm IEC 60127 2 250VAC Time Lag T Low Breaking Capacity Glass Tube At 110 120 VAC Rating 0 2A 200mA Examples Schurter 0034 3110 At 220 240 V...

Page 35: ...g on abrasive plate used Grinding Surface Variation Less than 1 0 m Grinding Speed 60 RPM Beveling Angle Range 5 90 degrees adjustable Micromanipulator Coarse drive 0 075 in dial revolution Fine drive...

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Page 37: ...eling by monitoring bubble size19 grinding procedure general information 17 Grinding Procedure 17 I Introduction 7 M mains fuses 3 32 Manipulator 9 models 8 Motorbase 8 N notes user 36 O operation pre...

Page 38: ...BV 10 OPERATION MANUAL REV 3 00 20180423 36 meter does not work 25 no beveling occurs 25 pipettes break during beveling 25 Troubleshooting 25 U Unpacking and setting up 13 W warranty 27 NOTES...

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