Spectradyne nCS1 Operation Manual Download Page 18 | Manualshive

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Spectradyne nCS1 Operation Manual Download Page 18

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Spectradyne LLC, All rights reserved

18

Version 2.5.0 Revision 3, July 2020

The Auto-analysis Engine

Automatic Processing of Raw Data

➢

Flagged acquisitions (of raw data, see Figure 8) are placed in the Auto-analysis Engine
queue for automatic processing into Spectradyne Stats files.

➢

Raw data is processed using standard parameters (refer to page 25 for details):

o

Peak Detection Threshold: 3.0 x the standard deviation of the baseline noise.

▪

NOTE: The peak threshold value should not be changed unless directed to
by Spectradyne.

➢

Control the auto-

processing engine using the “Pause”, “Go”

and “Skip”

buttons.

Figure 13

. Auto-analysis Engine Interface.

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Summary of Contents for nCS1

Page 1: ...Spectradyne nCS1 TM Operation Manual Spectradyne LLC www NanoparticleAnalyzer com...

Page 2: ...the Analyte into the Cartridge 7 Load the Cartridge into the nCS1 7 nCS1 Control and Data Acquisition Software 9 Software Startup 9 Enter Run Details 9 Initializing the Cartridge 10 Single click to Ac...

Page 3: ...a Analysis 19 After Data Acquisition 20 End Measurement Run 20 Prepare the Next Cartridge for Analysis if continuing with measurements 20 Remove the Used Cartridge 20 Insert the Next Analysis Cartridg...

Page 4: ...the Diameter and Concentration Scaling of the CSD 29 2 2 Filter the Set of Detected Particles to Exclude False Positives 31 3 Combine and Compare 34 3 1 Combine multiple Stats files into a single Com...

Page 5: ...rement RMM techniques A clean disposable cartridge targets a specific size range of particles for each analysis eliminating concerns of contamination between samples Specifications Dimensions 12 x 12...

Page 6: ...t USB connection Use the USB cable provided with the instrument to connect the nCS1 to a computer with Spectradyne software installed The USB port is located on the back panel Power on Turn on the ins...

Page 7: ...3 IMPORTANT Ensure that any air bubbles in the sample are acceptably positioned in the reservoir see Figure 2 Gently tap the cartridge on a flat surface to shift remove any bubbles if necessary Figur...

Page 8: ...he button ring light blue on the front panel of the instrument must be illuminated before proceeding Press the cartridge engage button on the front of the nCS1 to raise the cartridge into the instrume...

Page 9: ...Box Number First six digit date code printed on the cartridge box e g 190915 NN Save Location Use the folder icon to specify a location for saving data Data File Prefix Specify a prefix for all data...

Page 10: ...rn shown in Figure 6 When the routine is complete data acquisition will begin automatically Figure 6 Run Control frame and fill monitors status Troubleshooting Tip If the priming routine encounters an...

Page 11: ...isition Stop Point value is reached see page 18 In continuous mode each acquisition is automatically flagged for processing unless a significant number of defects are detected in the data see page 18...

Page 12: ...eline Drifting of the baseline is acceptable In some situations there may be occasional steps in the baseline These steps may be acceptable depending on their severity If the raw data shows too many s...

Page 13: ...e event of a blockage Press the Clear Constriction button Figure 9 and wait for the routine to complete Continue acquisitions as normal press Continuous on Acquisition panel How much data is enough Th...

Page 14: ...efrigerate running buffer if intended for future use o Add 25 50 mL of deionized water to running buffer bottles or separate DI water bottles and replace in the instrument Note Use only fresh deionize...

Page 15: ...pectradyne technician P1 IN port must always have the higher pressure setting than the rest of the ports To set the pressure at a specific port enter a PSI value max 10 psi into the corresponding entr...

Page 16: ...onding boxes see Figure 11 Click ON SET to apply the setting Check the bias slider to ensure balance Press the OFF button to turn off the voltage and turn on the fill monitor sensors Sensors IMPORTANT...

Page 17: ...and store it in a safe place for future use Drain Bottle Lines This automatic sequence drains any remaining liquid in the lines back into the bottles Click the Drain Bottle Lines button and follow th...

Page 18: ...ne queue for automatic processing into Spectradyne Stats files Raw data is processed using standard parameters refer to page 25 for details o Peak Detection Threshold 3 0 x the standard deviation of t...

Page 19: ...ements on the Live CSD o Specify an integration range using the Measure Size Range box entries provided and click Set o The measured concentration for a specific size range with statistical error is d...

Page 20: ...n of the End Run routine lower the stage by pressing the button on the front panel of the instrument and remove the cartridge The nCS1 is now ready for the next measurement run load a new cartridge fo...

Page 21: ...support spectradynellc com Optimal sample parameters Concentration 109 particles ml below 300 nm 108 particles mL above 300 nm Calibration beads optional Add calibration beads to final concentration 1...

Page 22: ...d processing parameters This step yields a quantitative Concentration Spectral Density CSDTM distribution 2 Refine Filter Any necessary refinement to the diameter scaling is performed e g to account f...

Page 23: ...Spectradyne LLC All rights reserved 23 Version 2 5 0 Revision 3 July 2020 Data Viewer Software User Interface Figure 15 Overview of the Spectradyne Viewer software user interface...

Page 24: ...of the plot options in the Raw Data Plot Parameters frame Raw display the raw data as it was collected Polished display the raw data after default signal processing PS Filtered display the raw data a...

Page 25: ...ains all the controls for processing raw data as well as relevant measured values for the selected raw data file The default processing parameter values are appropriate for use in most cases These def...

Page 26: ...ion tab Note Stats Files A Stats file shares the same base filename as the raw file from which it was generated with an additional extension of the form _ssNN In the extension NN is an integer that in...

Page 27: ...weighted or volume weighted size distribution and between concentration density or absolute concentration X Axis frame X Limits Specify the x axis limits Log X Plot in log scale on the x axis Binning...

Page 28: ...etermines whether data from a single Stats file or multiple Stats files will be displayed Figure 16 Inspecting individual peaks detected in the raw data Note Refine Data Group Any outlier Stats files...

Page 29: ...d to generate Stats files in the Currently Loaded files list These Stats files will contain measurements of many individual particles in the same sample under the same measurement conditions and can t...

Page 30: ...he default calibration and the user may wish to refine the diameter scaling to align known features of the CSD e g a peak of calibration particles with the appropriate diameter or concentration Proced...

Page 31: ...applied to be effective o Under Entire State click Selected or All to apply the loaded values to a single selected file or to all files Note Refining the diameter scaling using a known feature in the...

Page 32: ...s the baseline noise some false positives i e peaks in the raw data that do not likely represent real particles naturally are included in the Stats file data set see Figure 17 The goal of this step in...

Page 33: ...e range of 0 2 4 0 approx Signal to noise S N o S N 15 cartridge dependent o A general sense for an appropriate S N cutoff can also be obtained by inspecting peaks in the raw data Figure 17 and Viewin...

Page 34: ...parameter and include an infinite range of diameters Polygon Filter has 2D user defined polygonal boundaries similar to a gate commonly used in analysis of flow cytometry results o Select the filter p...

Page 35: ...bined file File name of the form prefix _YYYYMMDD_ccNN h5 where NN is an incrementing number unique for each day o A Run Report Same file name as Combined file with xlsx extension 3 2 Compare Multiple...

Page 36: ...0 D90 values corresponding to 10 50 and 90 of particles are available for display on the CSD In the Quantification tab of the Viewer select the size range of interest either graphically or by manually...

Page 37: ...el xlsx format file containing a complete summary of the measurement It contains o Meta information about the measurement o Graphical size distributions in linear and logarithmic y axes o Summary stat...

Page 38: ...Three formats are available for export Microsoft Excel format xlsx MS DOS CSV format csv Lumetics Link format lum For Excel format the file selected in the Currently Loaded files list is exported to...

Page 39: ...Spectradyne LLC All rights reserved 39 Version 2 5 0 Revision 3 July 2020 Cartridge Size and Concentration Coverage A more up to date cartridge size coverage may be found on our website...

Page 40: ...operation of the nCS1 and its software can be obtained by sending email to support spectradynellc com At Spectradyne we are eager to help with your particle analysis Please contact us for help with sp...

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