Smith-Root eDNA Sampler Instruction Manual Download Page 1

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11569.01 eDNA Backpack Sampler Instruction Manual

INSTRUCTION MANUAL

For eDNA Sampler videos and tutorials, visit:

www.smith-root.com/support/tutorials

Summary of Contents for eDNA Sampler

Page 1: ...11569 01 eDNA Backpack Sampler Instruction Manual INSTRUCTION MANUAL For eDNA Sampler videos and tutorials visit www smith root com support tutorials...

Page 2: ...s The restocking fee is figured at 10 of the purchase price but not less than 20 00 Customers receiving equipment in damaged condition will be referred to the shipping company for insurance reimbursem...

Page 3: ...ocation purchased separately 5 Long tubing for eDNA Sampler IN port 6 Short tubing for eDNA Sampler OUT port 7 Remote control for eDNA Sampler pump 8 Rechargeable batteries and charger for remote cont...

Page 4: ...t G GPS antenna H Remote Control Unit I Input power cable J Battery standalone sampler K Harness L Upper dry bag M Lower dry bag N Waterproof door Backpack unit only A B C D E F G H I J K M N L Backpa...

Page 5: ...e battery to eDNA Sam pler power cable 4 Fold down dry bag to cover the bat tery 5 Using a small screwdriver remove the water tight cover from remote control battery compartment Do not fully remove th...

Page 6: ...op Pump control Initiate or end the water filtration cycle 9 Log ID The event ID number in the csv data record 10 GPS signal strength 11 Remote control connection indicator 12 Current sample 13 Sample...

Page 7: ...ater in the filter and tubing when target ing specific sample volumes When the start button is pressed in Auto Mode a new sample ID and data record will be generated equivalent to pressing the Reset b...

Page 8: ...PSI 2 Minimum Flow Flow rate at which the Low Flow Alarm will sound 5 rapid beeps 3 Volume Offset Value deducted from target volume to alarm user when to invert filter 4 Display Mode Mode selection b...

Page 9: ...itting from the out tube with no bubbles 3 Stop the pump and press the reset button on the interface 4 Invert the filter housings by rotating pole while retaining as much water in the extension tubes...

Page 10: ...ipod 4 Extend the first telescoping pole sec tion section with clamp and run the long piece of tubing end with adapt er up through the guides to the end of extension near clamp 5 Connect other long tu...

Page 11: ...Continue filtering until 1 The system beeps 2 times indicating you need to remove nozzle to achieve target volume Auto Mode 2 The system beeps 5 times indicating that flow has dropped below a registr...

Page 12: ...ing this procedure in the worst case scenario highest likely level of suspended particulate such as after a rain event Then a standardized sample volume e g 1L can be set for all samples knowing that...

Page 13: ...op the filtration cycle and try a larger pore size filter 7 Allow the pump to run while mon itoring the Flow Rate graph on the interface Once the pressure limit is reached the flow rate will begin to...

Page 14: ...lding process see above 3 Label the sample with an ID and place the preserved sample in a storage box Samples can then be stored in the dry bags of the eDNA Sampler unit 4 Record the Log ID from the i...

Page 15: ...backpack and walking along a transect The system uses GPS to plot the eDNA filtration transect while also giving the user a constant output of ml meter thus allowing for standardization of volume fil...

Page 16: ...nterface to start a new data record 2 If the environment will require you stop and start the pump repeatedly to avoid obstructions or shallow areas program the eDNA Sampler to Man ual mode In Auto mod...

Page 17: ...much water in the extension tubes as possible 3 Move around the obstruction to the next point in your sampling transect 4 Press the Start Stop button while simultaneously flipping the pole back into t...

Page 18: ...does not need to be sterilized between each sample However when moving between watersheds it may be advisable to ster ilize the system to avoid transferring bacteria or pathogens between sensitive sys...

Page 19: ...fully flush the system with fresh water prior to storage Note Do not use bleach concentrations 2 as this will damage the pump b Run the pump in manual mode at 1L min for 2 minutes but do not run the...

Page 20: ...ransfer the data logs to the USB drive 3 When prompted remove the USB drive from unit A separate csv file is created for each sampling event Log ID To locate the data associated with a particular samp...

Page 21: ...ting with pump Change the remote control batteries It s advised to carry spare AA batteries and a 1 Phillips screwdriver when sampling TIPS AND TRICKS FOR LONG TERM OPERATION Follow the pump storage p...

Page 22: ...ample packet containing a 47mm self preserving filter housing and at tach the extension tube to the housing Save the foil pouch for subsequent filter storage 2 Attach suction tubing to the filter hous...

Page 23: ...raction 9 Once in the laboratory for DNA extraction remove the filter housing with the preserved 47mm eDNA membrane from the storage bag 10 Open the filter housing using the pull tab and reveal the eD...

Page 24: ...ions including bipod 41 5 1054 1mm to 144 3657 6mm L X 5 25 133 4mm D Telescoping pole weight with bipod 7 lbs 3 18kg Remote control dimensions 4 7 119 4mm L X 2 5 63 5mm W X 0 94 23 9mm H Remote weig...

Page 25: ...info smith root com 360 573 0202 Vancouver WA USA www smith root com Since 1964...