Sigma 147161 Operating Manual Download

Page: 1 / 91

Translation of the original operating manual

sb
10/2012
Rev. 1.22 of 08/05/2020
Sigma 4-16KHS; ;

part no. 0701802

Sigma 4-16KHS

from serial no. 147161

Refrigerated Centrifuge
with Heating System

Operating Manual

Please retain for later use!

Summary of Contents for 147161

Page 1: ...e original operating manual sb 10 2012 Rev 1 22 of 08 05 2020 Sigma 4 16KHS part no 0701802 Sigma 4 16KHS from serial no 147161 Refrigerated Centrifuge with Heating System Operating Manual Please reta...

Page 2: ...Refrigerated Centrifuge with Heating System Sigma 4 16KHS 2 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802...

Page 3: ...of 08 05 2020 sb 3 91 Translation of the original operating manual part no 0701802 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 F...

Page 4: ...Refrigerated Centrifuge with Heating System Sigma 4 16KHS 4 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802...

Page 5: ...2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety instructions...

Page 6: ...of a rotor 29 6 2 2 2 Installation of an angle rotor with a hermetic lid 30 6 2 2 3 Installation of accessories 32 6 2 2 4 Adapters 34 6 2 2 5 Vessels 34 6 3 Control system Spincontrol S 35 6 3 1 Use...

Page 7: ...8 1 3 Rotors buckets and carriers 64 8 1 4 Load bearing bolts 65 8 1 5 Glass breakage 66 8 2 Sterilisation and disinfection of the rotor chamber and accessories 66 8 2 1 Autoclaving 67 8 3 Service 68...

Page 8: ...Refrigerated Centrifuge with Heating System Sigma 4 16KHS Table of contents 8 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802...

Page 9: ...a Standardm odule 010 Allgem eine Inform ationen 010 0020 Bestimm ungsgem e Verw endung Sigma 26 m od_1405318951312_68 docx 191709 2 1 1 2 Intended use Centrifuges are power driven machines that separ...

Page 10: ...dmodule 010 Allgemei ne Informati onen 010 0050 N ormen und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declarat...

Page 11: ...n Pos 16 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 020 Aufbau und Wirkungsw eise 020 0010 Aufbau der Zentrifuge 26 m od_1405318982945_68 docx 191793 2 1 2 1 Layout of the centrifuge Pos 17 10...

Page 12: ..._4 16KH S 020 Aufbau und Wirkungsw eise 020 0010 0020 Typenschild 4 16KHS 29 mod_1405326892357_68 docx 198625 3 1 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5...

Page 13: ...cally as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor...

Page 14: ...rifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram prov...

Page 15: ...1378 2 1 3 1 Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma Dangerous voltage I On Power Hot surface 0 Off Power Caution Risk of bruising Name pl...

Page 16: ...es and symbols to indicate hazards are used DANGER This symbol stands for a direct hazard to the life and health of persons Non observance of these symbols causes serious health problems up to life en...

Page 17: ...y The centrifuge has to be maintained regularly see chapter 8 Maintenance and service Components that are not in a perfect state must be replaced immediately Pos 37 010 U niversalmodule Leerzeile 0 m...

Page 18: ...g in compliance with the relevant standards Pos 44 010 U niversalmodule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 45 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0...

Page 19: ..._0 docx 114 1 Pos 49 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0040 C hemische und bi ologische Sicher hei t 26 m od_1405319028141_68 docx 192101 3 1 3 6 4 Chemical a...

Page 20: ...bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of a rotor Observe the instructions on the installati...

Page 21: ...ion cracks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service lif...

Page 22: ...bucket receives a batch number enabling conclusions to be drawn concerning the production process and the subsequent quality inspection Some rotors also have an additional serial number providing furt...

Page 23: ...og box see chapter 7 2 Table of error codes Pos 66 010 U niversalmodule Leerzeile 8pt 12 mod_1337063062114_0 docx 65687 1 Pos 67 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030...

Page 24: ...gen Sigma Standar dmodule 030 Sicher heit 030 0100 R estrisiken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The centrifuge was built in accordance with the state of the art and in co...

Page 25: ...m od_1405319161442_68 docx 192367 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage tem...

Page 26: ...Lagerung und Tr ansport 040 0050 Transportsicherung 4 16KS_KHS berschrift 29 mod_1405326914634_68 docx 198709 2 1 4 5 Transport safety device Pos 90 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 4 16K...

Page 27: ...s condensational water will collect inside the centrifuge It is important to allow sufficient time for drying min 24 h before the centrifuge can be used again Pos 95 010 U niversalmodule Leerzeile 0 m...

Page 28: ...eits au er 3 30KS 7 mod_1306312302397_68 docx 43513 3 1 5 2 2 Customer provided fuses Typically the centrifuge must be protected with 16 Amp L or B fuses that are to be provided by the customer NOTE T...

Page 29: ...1405319202508_68 docx 192465 3 1 6 2 1 Opening and closing the lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the l...

Page 30: ...x 114 1 Pos 113 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S 060 0020 0020 0023 Einsetzen eines Wi nkelrotors mit H ermetikdeckel 10 Nm 29 m od_1405326759765_68 do...

Page 31: ...original operating manual part no 0701802 Loosen the rotor tie down screw anti clockwise and remove the rotor together with the lid Loosen the lid by turning it anti clockwise and then remove it 1 Ro...

Page 32: ...ation of accessories Only use vessels that are suitable for the rotor In the case of swing out rotors all of the compartments must be equipped with buckets Always load rotationally symmetrical compart...

Page 33: ...loaded evenly It is not permissible to load angle rotors on only one axis correct incorrect Fig 9 Permissible and impermissible loading of an angle rotor and a swing out rotor example illustrations N...

Page 34: ...S 060 0020 0020 0051 Gef e 4 16S 4 16KS 4 16KHS 56 mod_1521541840863_68 docx 559986 4 1 6 2 2 5 Vessels Load the vessels outside of the centrifuge Liquids in the buckets or multiple carriers cause cor...

Page 35: ...mod_1405320193718_68 docx 193123 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several d...

Page 36: ...ifuge lid must be opened before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is t...

Page 37: ...he actual speed is displayed below this value The values are stated in revolutions per minute min 1 rpm and they depend on the RCF values see chapter 2 2 2 1 Speed radius and relative centrifugal forc...

Page 38: ...peed is reached Select the field Runtime and press the function knob The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 cloc...

Page 39: ...unction knob in order to accept the data Fig 14 Rotor selection list here for a swing out rotor Automatic rotor identification The centrifuge automatically identifies the rotor that is currently being...

Page 40: ...Standard menu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode...

Page 41: ...No message will be issued when the set temperature is reached A parameter is changed except for the temperature or another input is made In this case the RAPID_TEMP program will be aborted No message...

Page 42: ...runtime of the running centrifugation run For this purpose a green progress bar and percentage value are displayed in the program field Fig 17 Progress indicator during a centrifugation run After the...

Page 43: ...me and temperature exception the RAPID_TEMP program will not be listed The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be display...

Page 44: ...still Depending on the substances that are to be centrifuged it may make sense to bring the centrifuge to a certain temperature prior to starting a centrifugation The temperature adaptation prevents t...

Page 45: ...ward facing arrow in the speed field Values between 1 2 and 10 0 g cm3 are possible Centrifugation monitoring The centrifugation monitoring function enables the continuous monitoring of the speed and...

Page 46: ...ed if the current temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message wil...

Page 47: ...he Save function will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be displayed in the status line and the lock...

Page 48: ...ge It enables the optimum adaptation of the centrifuge to its specific area of application Fig 22 Menu Setup Function Open lid after run The automatic lid opening function ensures that the lid opens w...

Page 49: ...erature can be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follo...

Page 50: ...e entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in order to enter the next cha...

Page 51: ...u The curve editor will be displayed Select a curve number between 20 and 29 in the input field Curve If the curve number is already used the stored curve will be displayed The input field Int is used...

Page 52: ...e since the necessary slope 0 03 min 1 rpm which means that it is beyond the defined range Example 2 Start speed 0 min 1 rpm final speed 15000 min 1 rpm runtime 10 sec not possible since the necessary...

Page 53: ...BA Zentrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S Spincontrol S Zent7 060 0030 0030 Programmbetri eb 27 m od_1405320235093_68 docx 193459 3 1 6 3 3 Program mode A program contains a...

Page 54: ...to confirm the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm it The program will be...

Page 55: ...list and confirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The program will be executed and...

Page 56: ...n in the Setup menu Fig 29 Program rotation function When a program is loaded while the program rotation function is active this program will be used as the start program for the rotation After the co...

Page 57: ...ding on the centrifuge type partly standard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 250V max 6 A part no 17702 Pos 160 010 Universalmodul e Leerzeile 0 mod_12021162445...

Page 58: ...nnection Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illuminated Several possible causes Power off on If the error occurs again contact servic...

Page 59: ...he opening on the left side of the control panel e g with a screwdriver Fig 31 Position of the opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole T...

Page 60: ...centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance err...

Page 61: ...ent of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma z...

Page 62: ...al stress e g by heat generators Pos 175 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 176 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 080 War tung und Instandhaltung 080...

Page 63: ...m odul e 080 War tung und Instandhaltung 080 0010 0020 Zubeh r au er 8K 28 m od_1405320618247_68 docx 194845 3 1 8 1 2 Accessories CAUTION For the care of the accessories special safety measures must...

Page 64: ...elfachtr ger au er Basic 28 mod_1405320621063_68 docx 194873 3 1 8 1 3 Rotors buckets and carriers Rotors buckets and carriers are produced with the highest precision in order to withstand the perman...

Page 65: ...t of the buckets and therefore the smooth operation of the centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bol...

Page 66: ...Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the gre...

Page 67: ...Accessories Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 3...

Page 68: ...o withstand this high level of stress high quality components were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especial...

Page 69: ...ment we are obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and sp...

Page 70: ...afe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new pack...

Page 71: ...ermissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of dec...

Page 72: ...requirements Electr connection Protection class IP code see name plate I 20 Power consumption kW Input fuse AT 2 0 16 0 at 220 240 V 50 Hz temperature fuses Performance data Max speed rpm Max capacit...

Page 73: ...y of air 80 from 5 C up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 211 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx...

Page 74: ...ith a data sheet that includes important information and notes on safety This data sheet must be added to the operating manual Pos 218 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 P...

Page 75: ...l operating manual part no 0701802 Pos 222 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 110 Anhang 110 0020 Dr ehzahl Schwer efeld Diagr amm 28 m od_1405320705642_68 docx 195153 2 1 11 2 Speed...

Page 76: ...ope of the fixed acceleration curves defines the time that is required to accelerate the rotor by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maxim...

Page 77: ...Appendix Version 10 2012 Rev 1 22 of 08 05 2020 sb 77 91 Translation of the original operating manual part no 0701802 Fig 39 Diagram of linear curves Fig 40 Diagram of quadratic curves Pos 225 010 Uni...

Page 78: ...dition 1 16K Edition 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 1...

Page 79: ...1 4 4 1 1 Acetone C3H6O 100 1 1 4 1 1 4 4 1 4 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Alumini...

Page 80: ...iaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylanili...

Page 81: ...2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1...

Page 82: ...30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric...

Page 83: ...v 1 22 of 08 05 2020 sb 83 91 Translation of the original operating manual part no 0701802 Pos 230 100 Sigma 110 BA Zentrifugen Sigma Proj ekte 4 16KS_4 16KHS 110 Anhang 110 0060 Konformit tserkl r un...

Page 84: ...Refrigerated Centrifuge with Heating System Sigma 4 16KHS 11 Appendix 84 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802...

Page 85: ...d Centrifuge with Heating System Sigma 4 16KHS 11 Appendix Version 10 2012 Rev 1 22 of 08 05 2020 sb 85 91 Translation of the original operating manual part no 0701802 11 7 Declaration of conformity C...

Page 86: ...ated Centrifuge with Heating System Sigma 4 16KHS 11 Appendix 86 91 Version 10 2012 Rev 1 22 of 08 05 2020 sb Translation of the original operating manual part no 0701802 Ende der Liste f r Textm arke...

Page 87: ...trifugation monitoring 45 Centrifugation of infectious toxic radioactive or pathogenic substances 19 Centrifugation principle 13 Centrifugation with low capacity 33 Centrifugation with vessels of vari...

Page 88: ...he symbols and notes 16 Explosive substances 19 External signal 50 F Filling quantity refrigerant 72 Fire preventions 19 Form for the return of defective parts 69 Function 48 Functional and operating...

Page 89: ...n mode active 36 Motor error 60 Motor shaft 29 30 62 Multiple carrier 34 N Name plate 11 12 No indication on the display 58 No power in the mains supply 58 Noise level 72 Nominal voltage 12 Notes on s...

Page 90: ...instructions for centrifugation 20 Safety of rotors and accessories 21 Safety chemical and biological 19 Safety electrical 18 Safety mechanical 18 Safety conscious work 17 Saving a program 54 Scope of...

Page 91: ...r chamber 23 Temperature monitoring 46 Temperature monitoring system 23 Temperature range 72 Temperature unit 49 Temperature value not reached 58 Thermal stress 62 Time fine 49 Time range 72 Toxic sub...

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