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OPERATION

 

T

HEORY OF 

O

PERATION

 

Refrigerated incubators are engineered to provide constant temperature incubation environments 
suitable for BOD applications. The unit can obtain a stable, uniform temperature in its chamber, 
ranging from 0°C up to 40°C. 

 

Achieving and Maintaining the Temperature Set Point 

When the unit is powered, its refrigeration compressor runs continuously. The chamber temperature 
controller is wired to a solid-state temperature probe located in the chamber airstream on the chamber 
back wall. When the controller detects that the chamber temperature has dropped below the set point, 
it pulses power to a heating element. The element is located adjacent to the compressor chiller coil in 
an air recirculation duct at the bottom of the unit. 

The processor employs proportional-integral-derivative analytical feedback-loop functions when 
measuring and controlling the chamber air temperature. PID-controlled heating pulse intensities and 
lengths are proportional to the difference between the measured chamber temperature and the 
current set point. The frequency of pulses is derived from the rate of change in that difference. The 
integral function slows the rate of pulses when the temperature nears the set point to avoid 
overshooting. 

A circulation fan provides even air distribution throughout the chamber and plays an important role in 
maintaining temperature uniformity around the shelf space. 

 

Temperature Low Limit Cutoff 

The refrigeration system comes with a cut off limit set at 1°C. This helps prevent the chamber 
temperature from dropping below freezing and endangering samples and fluid-filled sample containers 
in the chamber. 

 

The Over Temperature Limit System (OTL

The OTL is a backup mechanical heating control system that operates independently of the 
microprocessor controller. When set, the OTL prevents runaway heating in the event of a failure of 
the main temperature control system or an external heat spike by rerouting power away from the 
heating element. 

The system consists of a mechanical thermostat control wired by a fluid capillary to an independent 
hydrostatic temperature probe located on the back wall of the incubation chamber. The OTL is set 

by the end-user

 at approximately 1°C above the current chamber temperature when operating 

stabilized at your application temperature. 

Because of its nature as a cutoff and its lack of PID analytics, the OTL cannot deliver the same 
degree of temperature stability and measurement precision as the digital display and controls. The 
OTL System should only be used as a means of heating regulation for the incubation chamber until 
samples can be safely transferred to another incubator. 

Summary of Contents for SRI3

Page 1: ...BOD INCUBATOR 110 120 Voltage 220 240 Voltage Installation Operation Manual SRI3 SRI3 2...

Page 2: ...er This unit is not designed for use with fruit flies Improper use of this unit including use with fruit flies will void any warranty Other units are specifically manufactured for fruit fly applicatio...

Page 3: ...ollowing standards for use within an ambient air pressure range of 22 14 31 3 inHg 75 106 kPa with no flammable volatile or combustible materials being heated CAN CSA C22 2 No 61010 1 2012 CAN CSA C22...

Page 4: ...djust Shelves 17 Deionized and Distilled Water 18 Installation Cleaning and Disinfection 18 GRAPHIC SYMBOLS 19 CONTROL PANEL OVERVIEW 21 OPERATION 23 Theory of Operation 23 Put the Incubator into Oper...

Page 5: ...rator or their supervisors must draw up a site specific standard operating procedure SOP covering each application and associated safety guidelines This SOP must be written and available to all operat...

Page 6: ...1 800 322 4897 extension 4 or 503 640 3000 FAX 503 640 1366 Sheldon Manufacturing INC P O Box 627 Cornelius OR 97113 ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its pro...

Page 7: ...t chamber through the door space Select thermocouples suitable for the application temperature you will be calibrating at Why Probes Reference readings taken outside the chamber using wire temperature...

Page 8: ...8 P a g e INTRODUCTION...

Page 9: ...and exterior If you find any damage to the unit follow the carrier s procedure for claiming damage or loss 1 Carefully inspect the shipping carton for damage 2 Report any damage to the carrier servic...

Page 10: ...10 P a g e Figure 1 SRI3 SRI3 2 RECEIVING YOUR UNIT ORIENTATION PHOTO Control Panel Temperature Probe Wire Shelf 1 of 2 Chamber Door Incubation Chamber Door Gasket...

Page 11: ...The data plate contains the unit model number and serial number Tech Support will need this information during any support call Record it below for future reference The data plate is located near the...

Page 12: ...12 P a g e RECEIVING YOUR UNIT...

Page 13: ...supply is present page 15 Install the Incubator in a suitable location Review the lifting and handling instructions page 16 Install the incubator in its workspace location page 16 Make sure the incub...

Page 14: ...units are built for use indoors at room temperatures between 15 C and 30 C 59 F and 86 F at no greater than 80 Relative Humidity at 25 C 77 F Operating outside these conditions may adversely affect th...

Page 15: ...ge varies more than 10 The wall power source must be protective earth grounded Use a separate circuit to prevent loss of the unit due to overloading or circuit failure The wall power source must confo...

Page 16: ...uate for lifting or stabilization Restrain the unit completely while lifting or transporting so it cannot tip Remove all moving parts such as shelves and trays and lock doors in the closed position du...

Page 17: ...is used to secure the shelves during transit and prevent damage to the chamber interior 1 Remove all shipping materials from the shelving Optional Shelf Adjustment Move the shelves as needed for your...

Page 18: ...0 uS cm for cleaning applications INSTALLATION CLEANING AND DISINFECTION The manufacturer recommends cleaning the shelving and unit chamber prior to installation of the shelving in the chamber The un...

Page 19: ...ge de la temp rature Over Temperature Limit system Thermostat temp rature limite contr le haute AC Power Rep re le courant alternatif I ON O OFF I indique que l interrupteur est en position marche O i...

Page 20: ...20 P a g e GRAPHIC SYMBOLS...

Page 21: ...rrent incubator air temperature accurate to 0 1 C The Up and Down buttons are used to navigate to the temperature set point and calibration adjustment display modes The display blinks continually whil...

Page 22: ...22 P a g e CONTROL PANEL OVERVIEW...

Page 23: ...to avoid overshooting A circulation fan provides even air distribution throughout the chamber and plays an important role in maintaining temperature uniformity around the shelf space Temperature Low...

Page 24: ...uracy verification procedures as well as any resulting calibration Perform the following steps and procedures to prepare the incubator for use each time it is installed in a new workspace environment...

Page 25: ...ment mode after 5 seconds of inactivity with the last shown set point value saved Set Point Adjustment Mode Initial Set Point 3 Adjust the set point to your application temperature using the Up or the...

Page 26: ...he unit temperature stability Check if any powered accessories in the incubation chamber are generating heat If you find no sources of external or internal temperature fluctuations contact Technical S...

Page 27: ...BILITY AND POWER OUTLET Outlets The SRI3 is provided with 2 power outlets inside the chamber Do not attach powered equipment drawing more than 1 amp from both outlets The SRI3 2 is provided with 1 pow...

Page 28: ...oint up and down in small increments If the relative humidity in the incubation chamber is already near the dew point barometric fluctuations may push it across the dew point threshold Note Thin air a...

Page 29: ...less steel surfaces Do not use chlorine based bleaches or abrasives these will damage the chamber liner Warning Exercise caution if cleaning the unit with alcohol or flammable cleaners Always allow th...

Page 30: ...an inhibit growth or cause metabolic symptoms in microbiological sample populations Make sure that chlorines quaternary ammonias or any other overtly volatile disinfecting agents have been rinsed or o...

Page 31: ...below freezing This happens frequently when the unit is run toward the low end of its performance range At these temperatures humidity in the air will condense and freeze on the coil Figure 3 Refrige...

Page 32: ...ates it insulates the heat transporting coolant fluid within the coil driving an increasing loss of chilling efficiency This may even cause false high temperature readings on the defrost thermostat an...

Page 33: ...ith the door open for 24 hours 2 Wipe up any condensate that forms in the chamber as well as any water around the incubator after 24 hours have elapsed 3 Close the chamber door plug the incubator powe...

Page 34: ...nd use the calibration setup required by your industry requirements or laboratory protocol Suggested Single Point Offset Calibration Setup Non stick tape recommended 1 Introduce the reference device t...

Page 35: ...ture Calibration 1 Once the incubator temperature has stabilized compare the reference device and incubator temperature display readings If the readings are the same or the difference between the two...

Page 36: ...ber temperature value 4 Use the Up and Down buttons to adjust the current display temperature value until it matches the reference device temperature reading Reference Device 5 After matching the disp...

Page 37: ...l range Reference Device 8 If the two readings are not the same and the difference still falls outside the acceptable range of your protocol repeat steps 3 7 up to two more times Three calibration att...

Page 38: ...38 P a g e USER MAINTENANCE...

Page 39: ...f the height width and depth of the inner chamber All indications are average values typical for units produced in the series We reserve the right to alter technical specifications at all times WEIGHT...

Page 40: ...SRI3 10 0 lb 4 5 kg 20 0 lb 9 0 kg SRI3 2 10 0 lb 4 5 kg 20 0 lb 9 0 kg TEMPERATURE Model Range Uniformity Stability SRI3 Ambient 0 C to 45 C 0 5 0 2 C SRI3 2 Ambient 0 C to 45 C 0 5 0 2 C POWER Mode...

Page 41: ...322 4897 extension 3 If you are uncertain that you have the correct Part Number or if you need that specific item please contact Sheldon Technical Support for help at 1 800 322 4897 extension 4 or 503...

Page 42: ...P O Box 627 Cornelius OR 97113 USA support sheldonmfg com sheldonmanufacturing com 1 800 322 4897 503 640 3000 FAX 503 640 1366...

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