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J1230 User Manual

Simons Electron Microscopy

Center (SEMC) at the New York

Structural Biology Center

(NYSBC)

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Summary of Contents for J1230

Page 1: ...J1230 User Manual Simons Electron Microscopy Center SEMC at the New York Structural Biology Center NYSBC ...

Page 2: ...Last updated on 9 20 19 1 Table of Contents J1230 Information Sheet 2 J1230 User Guide 3 Troubleshooting 7 Alignments 6 Microscope Recovery 7 ...

Page 3: ...t click drag will adjust When BEAM SHIFT lift right click drag to move beam When STEP is lit adjustments are coarse BRIGHTNESS Obj Focus Important software controls General controls needed for operation are circled DO NOT adjust other alignments without consulting staff first You can leave the Monitor panel open far right Change mag using MAG CAM L toggle switch Left down mag Right up mag ...

Page 4: ...il you can t any further h Wait for vacuum i Watch V37 and V34 turns from white closed to black open and then finally back to white closed during retraction i Wait for SPC to change from Vac ready to Air will go to the 200 range j Pull holder straight out you should feel no resistance 3 Transfer negative stain grid to holder tip 4 Insert holder a Objective aperture OUT flu screen DOWN stage recent...

Page 5: ... 300 um aperture g Run application J1230MSI Raster Screen2 3 2 i Main j1230leginon ii Scope and camera 1230 cam1 8 Import presets a J1Presets_Manager Import presets from another session i TEM Jeol1230 ii Digital Camera Gatan iii Find iv Load most recent users presets and select all v Import Done NOTE We DO NOT recommend taking a grid atlas b Leginon works best in a semi manual mode c If you must t...

Page 6: ...or fa and fc presets ii Note you may need to adjust beam intensity after shifting the beam d Lift screen close the furthest left panel on the scope monitor click screen to lift lower flu screen 12 Simulate square target a In the J1Square node b Click simulate target 13 Submit subsquare targets a In the J1Subsquare_Targeting node b Use the acquisition mouse pointer to select subsquare area of inter...

Page 7: ...t updated on 9 20 19 6 i Spc Pos Position click center of stage map make sure Point option is ON ii Tilt Rot selected click center of stage map f Retract holder g Remove grid and store holder inside column ...

Page 8: ...true focus and reset using MF display in Leginon Resume collection in J1Exposure node play button Exposure and Focus node should use Image Beam Shift to move to target Grid Square and Subsquare node should use Stage position to move to target YOU MUST USE THE J1230 Raster app STAFF USE ONLY Alignments Frequently you will need to adjust gun tilt and possibly gun shift after a vacuum crash You shoul...

Page 9: ...he vacuum panel and wait The RT reserve tank will evacuate upon reaching the trip level around 120 and then the gun and column will actively be pumped 5 When COL and GUN are in the 20s you can safely turn on the high tension by clicking the HT button on the top left corner of the screen Set HT to on This triggers the pumping of the camera chamber 6 When emission is around 46uA the microscope is re...

Page 10: ...Last updated on 9 20 19 9 ...

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