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SCIEX NanoSpray, Operator'S Manual

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NanoSpray

®

 III Head Troubleshooting Tips

Tip! 

To troubleshoot problems with the NanoSpray

®

 III head, remove the ion source from the mass spectrometer,

and then run sample through it.

Corrective Action

Possible Cause

Symptom

a. Refer to 

Assemble the NanoSpray

®

 III

Head

.

a. A loose connection.

b. In rare cases bubbles can result from

normal outgassing as the gradient moves
through a 1:1 solvent mix. They can also
be a sign of dead volume resulting from
an improperly installed emitter tip.

Air bubbles at emitter
tip

Operator Guide

NanoSpray

®

 Ion Source

RUO-IDV-05-0811-F

68 / 92

Troubleshooting

Summary of Contents for NanoSpray

Page 1: ...NanoSpray Ion Source Operator Guide May 2018 RUO IDV 05 0811 F ...

Page 2: ...nt and does not imply any right and or license to use or permit others to use such manufacturers and or their product names as trademarks SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are SCIEX s sole and exclusive representations warranties and obligations SCIEX makes no other warranty of any kind whatsoever expressed or impli...

Page 3: ... 14 3 Ion Source Components 15 Camera and Illuminator 16 Ion Source Head 18 X Y Z Positioning Unit 18 Positioning Rails 19 NanoSpray Interface Components 20 OptiFlow TM Interface Components 20 Requirements 22 Gases 22 Solvents 22 4 NanoSpray III Head Assembly and Installation 23 Assemble the NanoSpray III Head 24 Remove the NanoSpray III Head 26 Prepare the Sprayer Fitting 28 Insert the Emitter Ti...

Page 4: ...ms 57 Install the Monitor 57 Install the Monitor 4500 5500 6500 6500 and TripleTOF Systems 57 Install the Monitor on 3200 and 4000 Series Systems 59 Connect the Monitor Cables 60 Bake the Interface 61 Clean the Ion Source 62 Clean the Nano Cell Heater Assembly 62 Required Materials 62 Tools and Supplies Available from the Manufacturer 63 Clean the Assembly 64 7 Troubleshooting 66 Mass Spectrometer...

Page 5: ...C Connecting the Syringe Using PEEK lined Fused Silica Tubing 83 D Source Parameters and Voltages 87 E Glossary of Symbols 88 NanoSpray Ion Source Operator Guide 5 92 RUO IDV 05 0811 F Contents ...

Page 6: ...e ion source only if you have knowledge of and training in the proper use containment and evacuation of toxic or injurious materials used with the ion source WARNING Hot Surface Hazard Let the NanoSpray ion source cool for at least 60 minutes before starting any maintenance procedures Surfaces of the ion source become hot during operation WARNING Toxic Chemical Hazard Wear personal protective equi...

Page 7: ...ct the drain tubing to the mass spectrometer and the source exhaust drain bottle properly to prevent leaks Determine which chemicals have been used in the system prior to service and regular maintenance Refer to the Safety Data Sheets for the health and safety precautions that must be followed with chemicals SCIEX Safety Data Sheets can be found at sciex com tech regulatory Always wear assigned pe...

Page 8: ...y be used with the system CAUTION Potential System Damage Do not use any other fluid until confirmation is received from SCIEX that it does not present a hazard This is not an exhaustive list Organic Solvents MS grade acetonitrile up to 100 MS grade methanol up to 100 Isopropanol up to 100 HPLC grade or higher water up to 100 Tetrahydrofuran up to 100 Toluene and other aromatic solvents up to 100 ...

Page 9: ...age fluctuations 10 of the nominal voltage Transient overvoltages Up to the levels of Overvoltage Category II Temporary overvoltages on the mains supply Pollution degree Pollution Degree 2 External power supply rating 90 VAC to 264 VAC 47 Hz to 63 Hz 1 A Performance Specifications The system is designed to meet specifications under these conditions An ambient temperature of 15 C to 30 C 59 F to 86...

Page 10: ...l conditions recommended in the Site Planning Guide If the mass spectrometer and ion source are used in an environment or in a manner not prescribed by the manufacturer then the protection provided by the equipment might be impaired Unauthorized modification or operation of the mass spectrometer and ion source might cause personal injury and equipment damage and might void the warranty Erroneous d...

Page 11: ... throughout the world They can answer questions about the system or any technical issues that might arise For more information visit the SCIEX website at sciex com Laser Safety Information This section contains safety information for the laser used in the illuminator for the NanoSpray III ion source Laser Classification WARNING Laser Hazard Do not view the laser output with an optical instrument C...

Page 12: ...Wavelength stability 3 0 mW 0 15 mW Output power 1 fluctuation over 60 minutes Output power stability 25ºC Labels on the Ion Source In accordance with regulatory requirements all laser warning labels displayed on the ion source are documented in this guide Warnings and labels on the ion source use international symbols Location Definition External Labels External On the top cover CAUTION Visible a...

Page 13: ...cation of the aperture through which the laser beam emerges External On the top cover Laser Radiation Avoid direct eye exposure Class 3R laser product Max power 5 mW Wavelength 650 nm IEC 60825 1 NanoSpray Ion Source Operator Guide 13 92 RUO IDV 05 0811 F Operational Precautions and Limitations ...

Page 14: ...he ion source is intended for continuous sample throughput It typically uses an external nano LC pump with a nano LC column for separation although infusion can also be used Samples travel along the nano LC column to the ion source head and then through an open ended emitter tip The ion source has an X Y Z positioning unit that can be used to position the emitter tip relative to the curtain plate ...

Page 15: ...er Laser warning labels not shown are located on the top of this cover 1 Ion source interface 2 Release pin 3 Release latch 4 Camera Refer to Adjust and Focus the Camera 5 NanoSpray Ion Source Operator Guide 15 92 RUO IDV 05 0811 F 3 Ion Source Components ...

Page 16: ...tective end cap 11 Illuminator Refer to Adjust the Illuminator 12 Camera and Illuminator The camera is mounted on a rod connected to the ion source Use it to focus the image on the monitor and to reposition the image of the emitter tip and curtain plate An illuminator provides light for observing the curtain plate aperture and the emitter tip Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 1...

Page 17: ...Figure 3 2 Camera and Illuminator Description Item Illuminator 1 Camera 2 NanoSpray Ion Source Operator Guide 17 92 RUO IDV 05 0811 F Ion Source Components ...

Page 18: ...ription Item NanoSpray III bracket 1 NanoSpray III head 2 X Y Z Positioning Unit After the X Y Z positioning unit shown in Figure 3 4 is in place against the ion source interface the position of the emitter tip can be adjusted with the X Y Z axis adjustment knobs The image shown on the monitor facilitates the adjustment of the emitter tip position Note The movement of the X Y Z positioning unit is...

Page 19: ...l movement 4 X Y Z positioning unit 5 Positioning Rails The ion source has two rails that support the X Y Z positioning unit Move the X Y Z positioning unit along these rails into and out of the operating position Moving the X Y Z positioning unit away from the ion source interface disconnects the high voltage supply from the ion source head and allows the ion source head to be removed The NanoSpr...

Page 20: ... the different mass spectrometers might be physically interchangeable but they have different aperture sizes Be sure to install the correct interface for the mass spectrometer Figure 3 5 NanoSpray Interface Components Description Item Curtain plate aperture 1 Curtain plate 2 OptiFlow TM Interface Components The ion source housing connects to the OptiFlow TM interface components Refer to Figure 3 6...

Page 21: ...re applicable only to the TripleTOF 6600 system Figure 3 6 OptiFlow TM Interface Components Description Item Nano cell heater assembly 1 Nano cell curtain plate 2 NanoSpray Ion Source Operator Guide 21 92 RUO IDV 05 0811 F Ion Source Components ...

Page 22: ...drocarbon content of less than 0 1 PPM and particle size of less than 0 01 microns Solvents For best results use high purity solvents for nano flow experiments Low quality solvents might result in high background contaminant peaks or blocked parts in the LC systems Contaminants are difficult to remove from the HPLC system and might result in downtime or require a service call We recommend pre made...

Page 23: ...1 NanoSpray III Head Components For assembly instructions refer to Description Item NanoSpray III bracket 1 Refer to Remove the NanoSpray III Headand Install the NanoSpray III Head on the Bracket High voltage rail 2 Straight union and holder 3 Refer to Connect the Sample Line Sample line connection 4 NanoSpray Ion Source Operator Guide 23 92 RUO IDV 05 0811 F 4 NanoSpray III Head Assembly and Inst...

Page 24: ...ips Uncut New Objective tips Pre packed column tips Note Emitter tips are also referred to as fused silica capillaries or needles Figure 4 2 shows the parts needed to assemble and install the NanoSpray III head The key indicates whether the parts are available in the Consumables kit Hardware Installation kit or both Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 24 92 NanoSpray III Head Ass...

Page 25: ...mm outside diameter o d 0 38 mm inside diameter i d 2 Both 1035752 Emitter tip precut 7 cm 3 Both 5015860 Finger tight PEEK hex nut 4 See 5015902 5016361 Union holder includes union PN 5015902 5 Consumables 5015902 Straight union 6 Both 5017932 Finger tight PEEK nut 7 NanoSpray Ion Source Operator Guide 25 92 RUO IDV 05 0811 F NanoSpray III Head Assembly and Installation ...

Page 26: ... robust syringe an alternate syringe PN 81075 is available from Hamilton Company Remove the NanoSpray III Head WARNING Electrical Shock Hazard Remove the ion source from the mass spectrometer before starting this procedure Follow all electrical safe work practices WARNING Electrical Shock Hazard Never operate the NanoSpray ion source without the illuminator camera stop and covers properly installe...

Page 27: ...nnect the supply of high voltage power from the sprayer head and high voltage rail 2 Pull the X Y Z positioning unit as far from the ion source interface as possible until it is stopped by the sleeve to disable the supply of high voltage power to the ion source head 3 Loosen the rail thumbscrew and then pull the high voltage rail back and up to remove it from the bracket Note Before the rail can b...

Page 28: ...itting before using the NanoSpray III head for the first time Refer also to the NanoSpray III Head and Emitter Tip Assembly Quick Start Guide CAUTION Potential System Damage Be careful when dismantling the NanoSpray III head because it contains small parts 1 Remove the sprayer fitting from the NanoSpray head assembly Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 28 92 NanoSpray III Head As...

Page 29: ... 4 4 with the end of the sleeve protruding 2 mm from the end of the ferrule Figure 4 4 Preparing the Sprayer Fitting 4 Insert the assembled fitting and the prepared sleeve into the rear of the sprayer head and then tighten slightly Figure 4 5 The Sprayer Fitting Installed NanoSpray Ion Source Operator Guide 29 92 RUO IDV 05 0811 F NanoSpray III Head Assembly and Installation ...

Page 30: ...mitter tips are delicate If the emitter tip is touched or bumped an emitter tip during installation throw it away and prepare a new one 2 Loosen the sprayer fitting 3 Insert the blunt untapered end of the emitter tip into the sprayer end of the NanoSpray III head and then through the sprayer fitting and green FEP sleeve as shown in Figure 4 6 Figure 4 6 Inserting the Emitter Tip 4 Loosen the union...

Page 31: ...work practices WARNING Electrical Shock Hazard Never operate the NanoSpray ion source without the illuminator camera stop and covers properly installed Never touch the curtain plate or allow the emitter tip to contact the curtain plate If the mass spectrometer is operational and the ion source is installed then high voltage is present on the curtain plate even if the X Y Z positioning unit is move...

Page 32: ... 3 Remove the stainless steel nut and ferrule from one end of the syringe union 4 Insert the needle and sleeve into the stainless steel nut and ferrule pushing the sleeve in as far as it will go and then insert the syringe needle 5 Use two 1 4 inch wrenches to tighten the nut 6 Insert one end of the fused silica tubing into the orange PEEK sleeve 7 Cut the end of the fused silica tubing and then c...

Page 33: ...raight union 13 Insert the fused silica tubing through the tan PEEK sleeve 14 Cut the end of the fused silica tubing and then clean it with a wipe dampened with methanol or isopropanol 15 Insert the sleeve and tubing into the PEEK fitting removed in step 12 until they protrude about 2 mm from the tip of the fitting NanoSpray Ion Source Operator Guide 33 92 RUO IDV 05 0811 F NanoSpray III Head Asse...

Page 34: ...ass spectrometer is operational and the ion source is installed then high voltage is present on the curtain plate even if the X Y Z positioning unit is moved away from the interface Adjust the illuminator and camera to obtain the best possible images on the monitor Adjust and Focus the Camera 1 Move the X Y Z positioning unit along the positioning rails away from the ion source interface until it ...

Page 35: ...milar to the recommended view shown in Figure 4 10 or Figure 4 11 The camera will be at approximately 40 degrees to the horizontal plane WARNING Electrical Shock Hazard Never operate the NanoSpray ion source without the camera properly installed Do not remove the camera Figure 4 10 View of Aperture Focus on the Orifice Region OptiFlow TM Interface Figure 4 11 View of Aperture Focus on the Orifice ...

Page 36: ...n positioning rail 5 4 Focus on the emitter tip by pushing the camera lens back in its holder or focus on the orifice plate by pushing the camera lens forward Adjust the Illuminator WARNING Laser Hazard Follow all local codes regulations standards and internal requirements applicable to laser safety Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 36 92 NanoSpray III Head Assembly and Install...

Page 37: ...G Electrical Shock Hazard Do not loosen or remove the movement control hex screws 3 Using fingers only adjust the position of the illuminator until the laser beam is aimed into the center of the aperture The illuminator should be aimed at the 4 00 position Note To see the illuminator look through the viewing ports in the cover Figure 4 13 Beam Aimed into Aperture OptiFlow TM Interface NanoSpray Io...

Page 38: ...µL min and 1 0 µL min CAUTION Potential System Contamination Do not insert the end of the emitter tip into the curtain plate aperture Make sure that the emitter tip is at least 2 mm to 5 mm outside the aperture Spraying too close to the aperture can cause contamination of the mass spectrometer 5 Adjust the X Y Z positioning unit until the emitter tip is visible on the monitor Operator Guide NanoSp...

Page 39: ...omes visible at the tip 7 Increase the IS or ISVF parameter until droplets spray towards the aperture in short bursts 8 Increase the GS1 parameter until the plume is visible Figure 4 16 and Figure 4 17 show the monitor image when the illuminator is adjusted correctly and the sample is flowing freely The position of the emitter tip might vary depending on the application Tip If the plume seems deta...

Page 40: ... NanoSpray Interface Note The picture is monochrome 9 Adjust the brightness and color on the monitor for best picture quality Inspect for Leaks WARNING Laser Hazard Follow this procedure exactly to avoid exposure to hazardous laser radiation Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 40 92 NanoSpray III Head Assembly and Installation ...

Page 41: ...flow 0 5 µL min to 1 0 µL min of solvent through the NanoSpray III head for a few minutes and then inspect the sleeves at all connections for visible leaks Note Smaller syringes can withstand greater back pressures 2 Remove the sample line fitting from the upstream side of the union 3 Examine the sample line to make sure that the sample is flowing If the sample is flowing then connect the sample l...

Page 42: ...d for example during replacement of the emitter tip set them to the optimized values being careful not to strike the emitter tip against the curtain plate This section describes how to optimize the performance of the NanoSpray III head for a specific compound Note For installation tests for the OptiFlow TM and NanoSpray ion source refer to the Ion Source Tests Specifications and Data Log available...

Page 43: ...the emitter tip position until the optimal signal and signal to noise ratio is achieved Note Do not set the IS voltage or ISVF too high Start with a low value and then adjust it upward If the voltage is too high then a corona discharge occurs detectable as a blue glow on the sprayer This can decrease the sensitivity and stability of the signal and damage the emitter tip 10 Set Ion Source Gas 1 GS1...

Page 44: ...propriate personal protective equipment and absorbent wipes to contain the spill and dispose of it following local regulations WARNING Electrical Shock Hazard Avoid contact with the high voltages applied to the ion source during operation Put the system in Standby mode before adjusting the sample tubing or other equipment near the ion source CAUTION Potential System Damage Do not lift or carry the...

Page 45: ...r free gloves nitrile or neoprene is recommended Lab coat Remove the Ion Source Tip Before removing the source note how the cables are routed so that they can be routed the same way when the source is installed The ion source can be removed quickly and easily without tools Always remove the ion source from the mass spectrometer before you perform any maintenance on the ion source 1 Stop any ongoin...

Page 46: ... the SelexION controller module The power switch is located on the back of the SelexION controller module 2 Align the ion source with the mass spectrometer Make sure that the ion source latches are in their unlocked positions that is the 12 o clock position and that they are aligned with the sockets on the mass spectrometer 3 Push the ion source towards the vacuum interface and then turn the ion s...

Page 47: ...lluminator and camera 7 Power and video harness 8 Illuminator switch box 9 Illuminator 10 12 VDC power supply cable 11 5 Connect the camera to the power and video harness 6 Plug the 2 pin locking plug on the power and video harness into the illuminator switch box 7 Connect the power and video harness to the 12 VDC power supply NanoSpray Ion Source Operator Guide 47 92 RUO IDV 05 0811 F Ion Source ...

Page 48: ...n the mass spectrometer If they are installed then the nano cell heater assembly and curtain plate must be removed and the standard curtain plate installed Refer to Change to a Different Ion Source OptiFlow TM Interface Components Note Because the orifice plate does not need to be changed the system need not be shut down and vented If they are not installed then the NanoSpray interface components ...

Page 49: ...no cell heater assembly separately and then store them in the nano cell holder 4 Install the standard curtain plate 5 Install the ion source Refer to the appropriate ion source Operator Guide Change to the NanoSpray Ion Source OptiFlow TM Interface Components 1 Remove the installed ion source Refer to the Operator Guide for the ion source 2 Install the OptiFlow TM interface components by following...

Page 50: ...Refer to the documentation that comes with the mass spectrometer Note The curtain plate is easier to remove from the interface when it is installed on the instrument c Install the NanoSpray interface components Refer to Install the Interface Components 3 Install the NanoSpray ion source Refer to Install the Ion Source Change the Interface Components Before using an ion source make sure that the co...

Page 51: ...ater assembly and the nano cell holder from the foam packaging 3 Remove the standard curtain plate on the mass spectrometer 4 Remove the nano cell curtain plate from the foam packing 5 Remove the nano cell heater assembly from the nano cell holder Figure 6 2 OptiFlow TM Interface Components Description Item Nano cell curtain plate 1 Nano cell holder 2 Nano cell heater assembly 3 NanoSpray Ion Sour...

Page 52: ...bly firmly into position Refer to Figure 6 3 Figure 6 3 Nano Cell Heater Assembly Contacts and Retaining Pins Description Item Retaining pins 1 Contact pins 2 Tightening sockets 3 8 Tighten the two retaining pins to secure the nano cell heater assembly 9 Install the nano cell curtain plate 10 Turn on the power for the SelexION controller module if SelexION technology is installed Remove the Interf...

Page 53: ...the mass spectrometer WARNING Hot Surface Hazard Let the ion source cool for at least 60 minutes for the NanoSpray interface before starting any maintenance procedures Surfaces of the ion source and the vacuum interface components become hot during operation 5 After the mass spectrometer reaches atmospheric pressure remove the installed ion source from the mass spectrometer and then put the ion so...

Page 54: ...e components on the mass spectrometer 1 Find the six contact pins on the interface components and the corresponding sockets on the mass spectrometer These pins and sockets act as keys to prevent the interface components from being installed in the wrong orientation 2 Orient the interface components so that the six contact pins align with the sockets when the two retaining pins are inserted in thei...

Page 55: ...rface components with both hands insert the retaining pins in the clamps and then push the assembly firmly into place If the interface components are properly aligned then a click is heard as the retaining pins are pushed into position NanoSpray Ion Source Operator Guide 55 92 RUO IDV 05 0811 F Ion Source Maintenance ...

Page 56: ...video and power cables from the monitor 4 Optional To remove the bracket from the mass spectrometer loosen the two 5 mm hex screws that hold the bracket on the mass spectrometer and then push the bracket up and pull it out to remove it 5 Loosen the thumbscrew that holds the monitor on the bracket and then remove the monitor from the bracket Figure 6 6 Bracket Assembly Description Item Thumbscrew 1...

Page 57: ...tor from the shelf Install the Monitor Install the Monitor 4500 5500 6500 6500 and TripleTOF Systems WARNING Electrical Shock Hazard Use only power supply model HES10 12010 0 7 with the NanoSpray III ion source On 4500 5500 6500 and 6500 series systems the monitor is supported on a bracket that can be installed on the left or right side cover On TripleTOF systems the monitor is supported on a brac...

Page 58: ...or 4500 5500 6500 and 6500 systems the configuration of the HPLC system determines where the monitor is installed Refer to the documentation provided with the HPLC system b If necessary insert the two 5 mm hex screws into the installation holes on the instrument cover and then tighten them slightly Make sure that the thread is still visible c Align the installation holes in the bracket with the ho...

Page 59: ...s 3 Thumbscrews 4 3 Connect the monitor cables Refer to Connect the Monitor Cables Install the Monitor on 3200 and 4000 Series Systems The monitor stands on an adjustable shelf 1 Install the monitor on the shelf The monitor is not fastened to the shelf so that the user can determine the best viewing position Note Make sure that the monitor is secured on the stand 2 Connect the monitor cables Refer...

Page 60: ...w connector at the other end of the video cable Note The cables supplied with the monitor include white and red connectors that are not used for this application These two wires can be removed to simplify the assembly Figure 6 9 Monitor Video Cable Connection Description Item Video cable to camera 1 Video cable to LCD monitor 2 RCA to BNC adapter 3 WARNING Electrical Shock Hazard Do not intentiona...

Page 61: ...heater assembly are clean and dry before baking the interface 1 Move the X Y Z positioning unit along the positioning rails away from the interface 2 Start the Analyst Analyst TF software 3 On the Navigation bar click Configure 4 Click Tools Settings Queue Options 5 In the Max Tune Idle Time field type 720 6 On the Navigation bar under Tune and Calibrate double click Manual Tuning Note Do not have...

Page 62: ...It is fragile Clean the surfaces of the ion source after a spill or when they become dirty Tip When removing the high voltage rail to reassemble the ion source head inspect for residue under the rail Use a slightly damp lint free wipe to remove any residue from under the rail and from the outside of the O ring Inspect the O ring for damage If it is damaged replace it with a spare 1 5 mm by 1 mm O ...

Page 63: ...pplies Available from the Manufacturer Part Number Description 1017396 Small poly swab thermally bonded Also available in the Cleaning kit 018027 Lint free wipe 11 cm x 21 cm 4 3 inches x 8 3 inches Also available in the Cleaning kit 5020761 Cleaning kit Contains the small poly swab lint free wipes Q0 cleaning tool cleaning brush and Alconox packets 5020761 Cleaning kit Contains the small poly swa...

Page 64: ...ining pins that secure the nano cell heater assembly Refer to Figure 6 10 Figure 6 10 Nano Cell Heater Assembly Contacts and Retaining Pins Description Item Retaining pins 1 Contact pins 2 Tightening sockets 3 4 Remove the nano cell heater assembly Note After the nano cell heater assembly and nano cell curtain plate are removed make sure that they are stored in the nano cell holder provided Operat...

Page 65: ...e aperture of the nano cell heater assembly 9 Repeat step 7 and step 8 three times 10 Wipe the nano cell heater assembly with a lint free wipe dampened with water 11 Wipe the nano cell heater assembly with a lint free wipe dampened with the cleaning solution Note If more rigorous cleaning is required then use the brush supplied in the cleaning kit 12 Wait until the nano cell heater assembly is dry...

Page 66: ... plug Refer to Figure 7 1 b Set GS2 to 0 in the acquisition method The Gas 2 inlet port on the ion source is blocked and Ion Source Gas 2 GS2 is set to a value other than 0 in the acquisition method Gas 2 Over Pressure error occurs and the electronics turn off Figure 7 1 Gas 2 Port Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 66 92 7 Troubleshooting ...

Page 67: ...haft with pump grease a The infusion pump is faulty Inconsistent flow a Increase the flow rate or use an LC pump a The syringe drive is unstable This occurs at low flow rates Sudden variations in pressure resulting in low or erratic TIC External Pump Troubleshooting Tips Corrective Action Possible Cause Symptom a Refer to the guide for the external pump to determine how to handle air bubbles or bl...

Page 68: ...ction Possible Cause Symptom a Refer to Assemble the NanoSpray III Head a A loose connection b In rare cases bubbles can result from normal outgassing as the gradient moves through a 1 1 solvent mix They can also be a sign of dead volume resulting from an improperly installed emitter tip Air bubbles at emitter tip Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 68 92 Troubleshooting ...

Page 69: ...and then clean it with isopropanol Note Do not cut damaged fused silica lined PEEK tubing It must be replaced Make sure that the sleeve size matches the o d of the fused silica Make sure that the ferrule used in the union is a high pressure ferrule e Confirm that the correct union is being used Sonicate or replace the union f Recut the blunt end of the emitter tip or recut the end of the sample li...

Page 70: ... less than 15 d Make sure that the rail is pushed forward as far as it will go a IonSpray TM voltage IS or ISVF is too low Droplets appear at the emitter tip when GS1 is set to 0 b The nebulizer gas GS1 setting is incorrect If it is too low the spray bends away from the curtain plate aperture If it is too high droplets appear In both cases sensitivity decreases c The Curtain Gas TM flow CUR is too...

Page 71: ...00 C f Inspect for leaks Refer to Inspect for Leaks g Inspect the gas connections on the ion source interface h Make sure that the rail is in position and that the thumbscrew is finger tight Check for tiny O ring inside the rail replace if it is damaged i Replace the liquid junction union a The emitter tip is not protruding the correct length b The IonSpray voltage IS or ISVF value is incorrect c ...

Page 72: ...Gas 1 d Reduce IS or ISVF The emitter tip might need to be replaced if it was exposed to too high a voltage a The tip is too close to the curtain plate b Too much of the tip is exposed c The incorrect gas is supplied for Gas 1 d IS or ISVF is too high Arcing can melt the emitter tip and damage the temperature controller board Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 72 92 Troubleshoot...

Page 73: ...tip is clogged or damaged Inability to view the spray plume on the monitor a Replace the emitter tip a There is dirt on the emitter tip Misshapen or incorrectly angled spray plume a Adjust the Source Gas parameters a Source Gas parameter GS1 IS or ISVF settings are incorrect No spray plume Monitor and Camera Troubleshooting Tips Corrective Action Possible Cause Symptom Refer to Adjust and Focus th...

Page 74: ...hree minutes while the tip is in the spraying position and the interface heater is on 50 C to 100 C When the sample flow stops move the X Y Z positioning unit away from the ion source interface or set IHT to 0 C f Reduce IHT to 50 C to 100 C a The incorrect gas is supplied for Gas 1 b Solvent quality is poor c The emitter tip and infusion line were not flushed before and after a Glu1 Fibrinopeptid...

Page 75: ...ze the source Refer to Assemble the NanoSpray III Head b Inspect for leaks Refer to Inspect for Leaks c Use the Instrument Optimization wizard to optimize the mass spectrometer d Inspect the sample concentration Use either fresh sample or sample that has been frozen Use an alternate sample such as 5600 Tuning Solution Renin or Reserpine to determine whether the sample is causing an issue e Trouble...

Page 76: ...rmic acid 10 acetonitrile b Clean or replace the syringe or sample line c Clean the curtain plate and orifice plate Contact the QMP If necessary bake the interface Refer to Bake the Interface If the problem is not resolved clean Q0 or the QJet ion guide following the procedures in the hardware documentation for the mass spectrometer a The diluent is contaminated b The syringe or sample line is dir...

Page 77: ... website at sciex com a The interface heater is faulty Temperature not reached a Open the Mass Spectrometer Detailed Status dialog For the NanoSpray interface the Source Temperature field does not contain the set temperature value and it is shown as n a and the Interface Heater Status should be Ready For the OptiFlow TM interface the Source Temperature field should contain the set temperature and ...

Page 78: ...edominant 1 As the solvent evaporates the electrical field increases and the ions move to the surface 2 At some critical field value ions are emitted from the droplets 3 Nonvolatile residue remains as a dry particle 4 Each charged droplet contains solvent positive ions and negative ions but with one polarity predominant The surface of each droplet contains an excess of charges As the droplets evap...

Page 79: ...charged droplets then enter a heated laminar flow chamber where they undergo a rapid evaporation with minimal thermal decomposition This gentle evaporation preserves the sample s molecular identity The laminar gas flow and the electric field between the heated chamber and the orifice plate aperture transports the ions into the vacuum system of the mass spectrometer The heated interface removes the...

Page 80: ...r without coated metal tips The charged solution is sprayed through a short piece of fused silica capillary or emitter tip Sample Solvent Composition Solvents that are mixtures of water and organic modifiers offer higher sensitivity than pure water or pure organic solvents alone The ideal solvent is a 20 1 mixture of water and an organic solvent together with a small amount of acid or base to act ...

Page 81: ...hing a signal adjust the distance from the curtain plate and the vertical position until the signal is optimized Do not change the head position after the signal is optimized Note Always monitor the signal and background levels while adjusting the sprayer position NanoSpray III Head Voltage Positive mode analyses usually require a voltage between 1500 V and 3000 V Negative mode analyses usually re...

Page 82: ...anoSpray interface or 300 C for the OptiFlow TM interface but this is too high for most applications For proteomics samples for example we recommend a temperature of 50 C to 150 C The interface takes approximately 10 minutes to reach its working temperature after the system reaches a vacuum ready state Refer to Source Parameters and Voltages The following describes the heater temperature set point...

Page 83: ...ica tubing follow the procedures in Figure C 1 up to and including Install the NanoSpray III Head on the Bracket Then continue with the procedure in this section This procedure provides instructions for using infusion If an HPLC system is being used then refer to the documentation for the HPLC system Figure C 1 Parts for the NanoSpray III Head NanoSpray Ion Source Operator Guide 83 92 RUO IDV 05 0...

Page 84: ...inch o d 0 030 inch i d 10 Both 1003988 100 µL syringe 11 Required Materials 2 5 mm hex screwdriver PN 1034765 PEEK tube cutter PN 011281 Fused silica cutter PN 1006143 1 4 inch and 3 16 inch wrench not supplied HPLC grade IPA or methanol not supplied CAUTION Potential System Damage Do not cut the PEEK lined fused silica tubing 1 Remove the fitting from the upstream side of the union 2 Insert the ...

Page 85: ... it will go and then use a 1 4 inch and a 3 16 inch wrench to tighten the nut Figure C 3 Syringe Union Ion Source Side 7 Cut a 3 cm length of the green PEEK sleeve 8 Insert the syringe needle in the green PEEK sleeve 9 Remove the stainless steel nut and ferrule from the other end of the syringe union 10 Insert the needle and sleeve into the stainless steel nut and ferrule pushing the sleeve in as ...

Page 86: ...interface slowly until it stops making sure that the emitter tip does not strike the curtain plate 14 Continue with Adjust the Illuminator Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 86 92 Connecting the Syringe Using PEEK lined Fused Silica Tubing ...

Page 87: ...drupole and QTRAP systems Curtain Gas TM flow CUR 25 TripleTOF systems 50 C to 150 C 75 C Interface heater temperature IHT Table D 2 NanoSpray III Head Negative Mode Range Typical Value Parameter 50 nL min to 2000 nL min 500 nL min Flow rate 2 mm to 5 mm 5 mm Sprayer distance from curtain plate 1000 V to 2800 V 1900 V Ionspray Voltage IS or Ionspray Voltage Floating ISVF 1 to 50 20 Nebulizer gas G...

Page 88: ... Authority ACMA EMC Requirements Alternating current Amperes current A Authorized representative in the European community Biohazard CE Marking of Conformity cCSAus mark Indicates electrical safety certification for Canada and USA Catalogue number Caution Note In SCIEX documentation this symbol identifies a personal injury hazard Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 88 92 E Glossa...

Page 89: ...te code on the label or product indicates the date of manufacture China RoHS logo The device does not contain toxic and hazardous substances or elements above the maximum concentration values and it is an environmentally friendly product that can be recycled and reused Consult instructions for use cTUVus mark for TUV Rheinland of North America Data Matrix symbol that can be scanned by a barcode re...

Page 90: ...ive to prevent electric shock Hot Surface Hazard In Vitro Diagnostic Device Ionizing Radiation Hazard Keep dry Do not expose to rain Relative humidity must not exceed 99 Keep upright Laser Radiation Hazard Lifting Hazard Manufacturer Moving Parts Hazard Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 90 92 Glossary of Symbols ...

Page 91: ...ial number Toxic Chemical Hazard Transport and store the system within 66 kPa to 103 kPa Transport and store the system within 75 kPa to 101 kPa Transport and store the system within 10 to 90 relative humidity Transport and store the system within 30 C to 45 C NanoSpray Ion Source Operator Guide 91 92 RUO IDV 05 0811 F Glossary of Symbols ...

Page 92: ...on USB 3 0 connection Ultraviolet Radiation Hazard Volt Ampere power VA Volts voltage V WEEE Do not dispose of equipment as unsorted municipal waste Environmental Hazard Watts W yyyy mm dd Date of manufacture Operator Guide NanoSpray Ion Source RUO IDV 05 0811 F 92 92 Glossary of Symbols ...

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