Sakura Histo-Tek VP1 Operating Manual Download Page 1

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Operating Manual

Summary of Contents for Histo-Tek VP1

Page 2: ...ydration defatting and paraffin infiltration of tissue specimens This instrument operates using independent systems so carefully read the sections on operating method replacement of consumables maintenance inspection of each part etc to ensure correct use Manufactured for Sakura Finetek U S A Inc Torrance CA 90501 Sakura Finetek Europe B V 2048 Alphen aan den Rijn NL Sakura Finetek Japan Co Ltd To...

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Page 4: ...n oven 1 9 2 Installing the spill tray for reagent bottle rack 1 9 3 Installing the activated carbon filter base and activated carbon filters 1 10 4 How to set the reagent bottle 1 11 5 How to attach labels 1 12 6 How to set the paraffin container 1 13 10 Turning on the Power 1 13 2 Explanation of the Instrument 1 14 1 Overview of the Instrument 1 14 3 Name of Each Part 1 15 4 Standard Accessories...

Page 5: ... 2 14 7 Pausing Aborting 2 15 1 Operations permitted during pause 2 15 Chapter 3 Cleaning 1 Cleaning 3 1 1 Type of Cleaning Operations 3 1 2 Starting Retort Cleaning 3 1 3 Starting Warm Water Flush 3 2 Chapter 4 Solution Exchange 1 Solution Exchange 4 1 1 Automatic Transfer 4 1 1 Starting the Automatic Transfer 4 1 2 Reset Solution Usage Information 4 2 1 Clearing the usage data 4 2 Chapter 5 Prog...

Page 6: ...ing station 6 8 8 Select Language 6 9 Chapter 7 Maintenance Inspection 1 Daily Care Inspection and Cleaning 7 1 1 Periodic Maintenance 7 1 2 Periodic Replacement 7 1 3 Recommendation of Warm Water Flush to Users of Neutral Buffered Formalin 7 1 4 Cleaning of the Paraffin Oven and Spill Tray 7 2 5 Cleaning of the Reagent bottle Rack and Spill Tray 7 2 6 Cleaning of the Reagent bottles 7 3 1 Removin...

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Page 8: ...ion may result in a serious injury of the operator or other person pertains to a potential hazard where failure to observe the specified instruction may result in damage to this instrument or other property or affect the process results pertains to an item to pay attention to or other useful information The symbols used on the labels attached to the instrument are explained The labels bearing one ...

Page 9: ... operation of the instrument lies primarily with the owner of the instrument and persons in charge who operate the instrument Do not install the instrument in a place used as a living area If a common area is used an exhaust system is required Provide electrostatic discharge protection in the installation location Install the instrument indoors Do not place the instrument in a location exposed to ...

Page 10: ...of usable reagents P 1 19 Do not put too much reagent or paraffin Reagent or paraffin may overflow and the processing may be discontinued Add no more than the specified amount of reagent or paraffin so as not to damage the tissues If buffered formalin is used be sure to clean the tubing regularly If the tubing is not cleaned the circuit may clog and result in failure Other problems may also occur ...

Page 11: ...njury of the user instrument failure etc may occur If foreign matter entered the instrument immediately stop the instrument and contact the Sakura Finetek Technical Support representative or local representative Do not operate the instrument while wearing metal accessories Conductors metal pen accessories etc worn on your body may contact the instrument and cause electric shock Do not block the in...

Page 12: ...the instrument is used If an inappropriate power cord or voltage is used instrument malfunction due to short circuit fire or voltage drop unexpected accidents or injuries due to electric shock etc can occur The preset voltage cannot be changed by the user For the power cord a standard cord specified by local law may have to be used If a power cord not specified by the PSE standard UL CSA standard ...

Page 13: ...t bottles or paraffin containers while the instrument is operating unless otherwise instructed in this manual Paraffin may leak and cause burns Poor reagent bottle connection reagent leak or failure may also occur If processing is suspended the tissues may be damaged depending on the situation Remember to resume operation Prolonged suspension may damage the tissues Keep the suspension time to the ...

Page 14: ...be obtained from the supplier manufacturer of the reagent You can also download it from the website of the supplier manufacturer Before enabling the automatic transfer function confirm that the first station for the applicable solution group is empty If the first station contains reagent empty the station Clean the bottle rack and spill tray regularly The paraffin temperature drops slightly when p...

Page 15: ...t apply excessive force on the display The display may break and cause injury Do not remove the warning caution labels Without these labels the operators cannot be reminded of the necessary warning cautionary information in the day to day handling of the instrument which may result in unexpected problems Do not put reagent etc on the instrument Also do not spill reagent on the painted surfaces cau...

Page 16: ...instrument Provide at least 10 cm of clearance around the instrument and make sure the power plug is installed so that it can be accessed at any time Thoroughly check the installation location before installing the instrument Install this instrument on a flat non slippery sturdy floor free from vibration and having a sufficient size If the installation location is tilted or too small unexpected ac...

Page 17: ...plug the power cord from the power outlet do not pull the cord but hold the plug and then pull it out Inspect the power cord periodically If any damage is found in the exterior of the power cord stop using it and contact the Sakura Finetek Technical Support representative or local representative The tissues may be damaged depending on the condition in which the error trouble occurred Immediately r...

Page 18: ...the combination of each container cassette etc and tissues is correct When storing tissues in a container cassette etc write down a short name or comment identifying the tissue information on the container cassette etc Be sure to do this to prevent serious trouble Verify beforehand that the letters written or printed on the containers cassettes etc in which tissues are stored will not be erased or...

Page 19: ...may occur due to clogged tubing etc If the melting point of paraffin is set to the preset temperature the paraffin may not melt completely depending on the environmental temperature If any reagent of low boiling point methanol ethanol etc is to be used for long hours of processing at high concentration use the P V OFF mode or heating OFF mode If kept under reduced pressure for a long period of tim...

Page 20: ... before storing the basket in the retort If the supplied basket is not used the tissues may not submerge in solution or they may float and may not be processed properly Use the specified supplies When replacing supplies use those specified in this manual Use of non specified supplies may result in malfunction or failure Reagent may scatter and attach to your body while working Be sure to wear prot...

Page 21: ...y each institution country or region Keep enough distance when opening the retort lid especially when the reagent is hot Also be careful not to inhale vapor Exercise due caution when handling reagents Follow the relevant regulations and guidelines in your country or region and take proper measures to protect the operator such as wearing protective gloves mask and or safety goggles Some reagents ar...

Page 22: ...riate measures to protect yourself as the operator Handling of reagents requires expert knowledge on associated hazards due to their toxicity and volatility etc Appoint a person responsible for reagents and use reagents under the guidance supervision of the responsible person If any additional requirement on accident prevention or environmental preservation is specified in the country region where...

Page 23: ...Chapter 1 Basic Knowledge of Instrument ...

Page 24: ...ic devices the instrument should not be installed in places subject to excessive temperature or humidity Temperature and humidity should be held at constant levels to achieve the highest degree of operating stability The ambient operating temperature of this instrument is in a range of 10 to 40 C Its ambient operating humidity is in a range of 30 to 85 in relative humidity non condensing Also loca...

Page 25: ...ument Type of power cord Applicable standard Length For 100 115 VAC UL CSA PSE approved product Approx 2 m For 220 VAC to 240 VAC Cord CENELEC HD VDE approved Plug EN IEC VDE Connector EN VDE approved Approx 2 m Warning If the instrument is used in a region where the applicable standard above is not met provide a different power cord conforming to the standard in the location of use About groundin...

Page 26: ...t to move it be careful not to let the exterior panels deform Push the corners of the exterior panels of the instrument including the top plate Two persons should work together to keep the instrument from tipping over When transporting the instrument place thin iron sheets etc to eliminate height gaps on the floor so that the instrument will not receive impact When transporting the instrument on a...

Page 27: ...ument may tip over or cause accident 1 Unpacking the instrument 1 Cut the two bands with a cutting knife etc and remove the cap 2 Remove the sleeve fixing bolts 4 pcs and take out the sleeves 3 Remove the accessory box protectors below the accessory box and monitor protectors 4 Untie the plastic strings and remove the L shaped cushions at the four corners and the cushions left right above the top ...

Page 28: ...bottle rack Open the Reagent bottle rack door and remove the two cushion materials 2 Removing the fixing members inside the instrument Remove the cushion materials and fixing members inside the instrument Refer to the explanation of how to open the door and retort lid Reagent bottle There is a blue part at the connection port coupler on the instrument side Push this part all the way into the instr...

Page 29: ...losing and locking the retort lid 1 Close the retort lid 1 2 Engage the tab of the hook with the metal part on the retort lid toward you 2 flip down the entire hook 3 and lock the retort lid The retort lid is locked at two locations so lock both locations Check to see if the retort lid can be opened 3 Slide the retort lock lever to the left 4 The cover on the hook moves to the left The hook is cov...

Page 30: ...or 1 To close the door raise the handle toward the back Caution Be careful not to pinch your hand If the door does not open it may be stuck due to paraffin Forcibly opening the door may cause a breakdown 6 How to operate the Reagent bottle rack door This is a double door Press the top of the door center of the instrument and you will hear a click sound and the door will open slightly toward you 1 ...

Page 31: ...are present refer to the table below and the instrument and accessories are free from damage If the instrument is damaged or any of the accessories is short or damaged contact your nearest Sakura Finetek dealer Name of part Q ty Remarks Basket with handle 150 cassette size 2 Basket set with dedicated lid Basket transport tray 1 Activated carbon filter 1 box Containing 2 units Divider for fume cont...

Page 32: ... pass through at the rear of the instrument the users may touch the power cord or hook it with their foot and accidentally cut off the power accordingly select a location where no unnecessary large space will be left at the back of the instrument 9 Installation Check Installing the Accessories 1 Installing the spill tray for paraffin oven Put the spill tray in the space below the paraffin oven and...

Page 33: ...ntrol unit into the instrument Set the unit so that the side with the larger hole comes to the front Make sure the front of the unit is inside the instrument If the front side is only resting at the entrance fume cannot be treated properly 3 Install the activated carbon filters to the instrument Two activated carbon filters constitute one set Fume cannot be treated properly if only one filter is s...

Page 34: ... open the door 2 Push the blue part at the connection port coupler on the instrument side toward the back of the instrument 1 and the coupler will be unlocked and you can now access the Reagent bottle Pull out the bottle horizontally 2 3 Close the Reagent bottle rack door Note Refer to p 2 3 for the solution level Where to set the bottles Processing bottles and cleaning bottles are set in the spec...

Page 35: ...ke sure it perfectly fits the concave and runs vertically to the bottle If the label deviates from the concave or is attached at an angle the solution level can no longer be checked properly How to attach color labels Labels in six colors total eight labels are provided to identify individual solutions Write down the solution names on the labels and attach them on the front face of the Reagent bot...

Page 36: ...iner 1 Open the paraffin oven door 2 While applying slight force gently and slowly pull out the paraffin container horizontally 3 Close the paraffin oven door Note For the paraffin volume refer to p 2 3 10 Turning on the Power 1 Confirm that input voltage has been set according to the installation environment 2 Connect the power cord to the inlet on the instrument and power outlet on the facility ...

Page 37: ...ng specimens can be processed in a single cycle Up to 10 tissue processing programs can be registered managed With the delayed start function you can set the instrument to wait and start tissue processing so that it will be finished at the specified date and time With the automatic transfer function you can let the instrument transfer reagents automatically instead of operators performing transfer...

Page 38: ...o the paraffin oven 7 Reagent bottle rack door Open this door to access the rack in which Reagent bottles are stored 8 Spill tray for Reagent bottle rack This tray receives the solution that has leaked into the Reagent bottle rack 9 Main power supply Circuit protector 1 Turn ON OFF the power to the instrument 10 Caster These casters are used for moving the instrument 11 Safety stoppers Caster stop...

Page 39: ...rating from solutions 22 Paraffin container Molten paraffin used for tissue processing is put in this container 23 Reagent bottle Solution used for tissue processing is put in this bottle See below for the name of each Reagent bottle 24 Reagent bottle rack Reagent bottles are stored in this rack 21 22 23 24 17 18 19 20 4 20 Front side of instrument Back side of instrument Handle on the top of the ...

Page 40: ...4 Standard Accessories Options 1 17 5 6 3 4 7 15 1 8 16 2 9 14 10 11 12 13 ...

Page 41: ...heets 12 Reagent bottle label for Cleaning O77 183 00 2 sheets 13 Color Coding Label 6 Colors x 4 Pieces sheet O77 184 00 2 sheets 14 Paraffin scraper Large 1550 1 each 15 Spill Tray for Paraffin Oven O77 165 00 1 each 16 Spill Tray for Reagent bottle Rack O77 168 00 1 each Screen Protection Sheet already attached to the screen O70 626 00 1 sheet Operating Manual 1 each Options Item Part number Q ...

Page 42: ... cleaning solution volume 4 5 to 4 8 L Fume treatment Activated carbon filters condenser Mixing method Pump in and pump out Display 10 4 inch color LCD touch screen Operating environment Required facility 100 VAC 10 50 60 Hz More than 15 A with grounding terminal 115 VAC 10 50 60 Hz More than 15 A with grounding terminal 220 240 VAC 10 50 60 Hz More than 10 A with grounding terminal Operating envi...

Page 43: ...he keyboard To delete characters from the entry field touch the Backspace key or the Clear key in the lower right of the keyboard The Backspace key deletes the currently displayed characters one by one The Clear key clears them at a time To save the current display touch Save To cancel the operation touch Cancel Touch keyboard 2 Using the ten key keypad The ten key keypad window is displayed to en...

Page 44: ...anagers and users Managers and users can perform different operations on this instrument Tab Details Manager ADMIN User OPERATOR Tissue Processing Select Program Tissue Processing Temporarily edit the program on the start confirmation screen x Temporarily edit the program during tissue processing x Utility Edit Solution Names x Edit Solution Configuration x Edit Program x Manual Operation Clean Re...

Page 45: ... groups can be set The user can set desired solution groups for Sta 1 to Sta 10 according to the actual state of tissue processing A solution group is defined as a group of stations of consecutive station numbers Station number Use Screen display 1 1 Solution for tissue processing Solution name 2 2 Solution for tissue processing Solution name 3 3 Solution for tissue processing Solution name 4 4 So...

Page 46: ... the header menu tab main screen and footer Tissue Processing Screen Delayed Processing Screen Utility Screen Solution Management Screen History Screen Standby Screen Header Menu tab Main Screen Footer Current date time Institution name log on user Logged on display mark ...

Page 47: ...2 Menu tab You can use the displayed menu tabs to move to the Tissue Processing Utility Solution Management and History screens 3 Main screen Each tab on the main screen shows different information and buttons 4 Screen footer The LOG ON LOG OFF button a message regarding the next operation the Caution button are shown in the footer area of the screen This icon is shown when a user is logged on as ...

Page 48: ...eparation process display area and information in the message display area Preparation process display area Processes needed to start tissue processing are displayed The current item is shown in yellow Select Program button The name of the program in use is displayed This is a button so when changing the program touch the program name and select a different program VIEW LIST Selector button Use th...

Page 49: ... exchange Paraffin Darker color Due for exchange Blinking Paraffin is not molten Tissue Processing button Select a program and touch this button and the instrument will proceed to the solution level check process Message display area Information of the next operation is displayed LOG ON LOG OFF button Touch LOG ON and enter the password the screen will accept operation inputs When the instrument i...

Page 50: ...er and solution name are shown below the retort Caution The solution level in the illustration is not accurate It may vary from the actual level Tissue processing program name The name of the program being processed is shown VIEW LIST Selector button Use this button to change the solution configuration display mode to list Touching the button again changes the mode back to station Tissue processin...

Page 51: ...e Paraffin Darker color Due for exchange Blinking Paraffin is not molten Pause button Touching Pause pauses the processing Touching Pause not only stops the tissue processing but it also resumes the tissue processing allows for temporary program edit and paraffin melt check and aborts the tissue processing When Pause is touched while the instrument is waiting for delayed start you can also start t...

Page 52: ... Configuration p 5 3 Select this icon to edit the solution configuration You can set solution groups as well as how to manage the use limits on reagent and paraffin You can also set when to perform warm water flush Edit Program p 5 6 Select this icon to edit and export tissue processing programs Manual Operation p 6 8 Select this icon to move through and fill drain stations Clean Retort p 3 1 Sele...

Page 53: ...lect this icon to allow for solution exchange at Sta 1 to Sat 10 based on automatic transfer Warm Water Flush p 3 2 Select this icon to perform warm water flush of Sta 1 to Sta 5 Export Data Touch this button to export the solution usage status Caution Connect the USB flash drive after touching the Export Data button Note Use an optional USB flash drive All Stations Touch this button to select or ...

Page 54: ...he History screen even during tissue processing Process Report p 6 1 Select this icon to confirm the process report Event log p 6 3 Select this icon to confirm the event log Error log p 6 3 Select this icon to confirm the error log Parts Usage p 6 4 Select this icon to confirm the parts usage Software Version p 6 4 Select this icon to show the version of the software ...

Page 55: ...Chapter 2 How to Operate Tissue Processing ...

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Page 57: ...Prepare solutions and paraffin Details p 2 3 Set solutions and paraffin Prepare for processing Details p 2 5 Select the tissue processing program Prepare tissue processing solution level check bottle connection check start check Run the process Details p 2 9 Set the specimens Start tissue processing Monitor tissue processing delayed start immediate start End the process Details p 2 13 Remove the s...

Page 58: ...rom the ten key keypad The default passwords are shown below Refer to p 1 21 for the differences between manager passwords and user passwords User ID User Name Password Manager 1 to 4 ADMIN 1 to 4 100000 200000 300000 400000 User 1 to 20 OPERATOR 1 to 20 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 To cancel the log on process touch Cancel The password entry screen will close and the previou...

Page 59: ...ent bottle and Cleaning Bottle Paraffin Container The required volumes of solutions and paraffin vary depending on the settings for mixing method and retort capacity Refer to the figure below for the required volumes 150 cassettes 300 cassettes Solution paraffin 2 0 L 3 5 L 3 2 L 3 5 L Cleaning 4 5 L 4 8 L Caution 4 5 to 4 8 L is required for cleaning Do not add solutions paraffin to above the MAX...

Page 60: ...reagent bottle side aligns with the most pointed part along the circumference of the bottle cap When the bottle cap is installed correctly the connection port of the bottle cap faces outward 5 Install the reagent bottle 2 Setting paraffin Add paraffin to the level on the scale corresponding to the required volume Refer to the table below for the volumes 150 cassettes 300 cassettes Paraffin 2 0 L 3...

Page 61: ...tissue processing Follow the messages in the preparation process display area on the left side of the screen to prepare for processing 1 Selecting the tissue processing program Select the program to be used for tissue processing 1 Touching Select Program 1 displays the problem list window 2 2 Select a program to be used for the tissue processing and touch Enter 3 To confirm the details of the prog...

Page 62: ...heck process When the solution levels in the reagent bottles were checked after the processing some bottles contained a lot more solution or full and other bottles were empty Contact the Sakura Finetek Technical Support representative or local representative Note If the program only uses paraffin stations automatic check is not performed and the Confirm Start window appears 3 Checking the performi...

Page 63: ...ther program To modify the program use the temporary program edit function Modifying temporary program To modify the program temporarily touch Temporary Program Edit 7 Refer to p 2 8 for details Note Modifications made under this function are temporary and effective only for the current run of tissue processing To use the modified program in future processing refer to Program Creation Editing p 5 ...

Page 64: ...d program temporarily Refer to p 5 7 for information on how to edit Note Modifications made under this function are temporary and effective only for the current run of tissue processing To use the modified program in future processing refer to Program Creation Editing p 5 1 Note Temporary program edit is not permitted if you have logged in with a non administrator password ...

Page 65: ...ch START to start processing If tissue processing will not be performed or if you want to check the program details again touch EXIT The Confirm Start window appears again Caution An unusual setting such as setting one cassette on the first basket and one cassette on the second basket may result in the top of the specimens set on the upper basket not submerged in the solution If there are fewer th...

Page 66: ...araffin is not filled drained to ensure safety The solution levels in the illustration are not accurate They may differ from the actual solution levels The following operations are permitted during delayed processing and tissue processing Delayed Processing Pauses the instrument and immediately start tissue processing Pauses the instrument and abort tissue processing Pauses the instrument and chan...

Page 67: ...m is displayed The station number processing time and solution name are shown at each station Each station is shown in the color preregistered for the solution group The station where processing is currently performed is shown in blue 3 View List Selector button Use this button to change the station configuration display mode to list Touching this button again to change the mode back to station 4 ...

Page 68: ...ram for the current program is displayed The station number processing time and solution name are shown at each station Each station is shown in the color preregistered for the solution group The station where processing is currently performed is shown in blue 3 View List Selector button Use this button to change the station configuration display mode to list Touching this button again to change t...

Page 69: ...is window appears Remove the specimens and drain the paraffin from the retort according to the message You can perform the two operations in any order Removing the specimens 1 Touch Take out basket 2 Follow the messages to open the retort lock cover remove the specimens from the retort and touch Yes Close the retort lid and close the retort lock cover and touch START to drain retort 3 When drainin...

Page 70: ...Before performing retort cleaning use the supplied paraffin scraper to carefully remove the deposits from the retort lid and areas around the retort and wipe off the scraped deposits using paper towels etc Be careful not to damage the edges of the retort lid gaskets with the paraffin scraper Use paper towels etc to wipe off deposits on the retort lid gaskets If retort cleaning is not performed wit...

Page 71: ...d during filling or protecting specimens All items can be edited during delayed processing If the current step has less than 1 minute remaining this step cannot be edited If the current station has 0 minute remaining not all processing times of the subsequent processes can be changed to 0 If the current station is not the last solution station and has 0 minute remaining not all processing times of...

Page 72: ...etort lock cover is open a warning window will appear and a buzzer will sound continuously During pause a buzzer will sound at 10 second intervals Aborting tissue processing Touch Abort in the Paused Processing window Touch Yes and the retort will be drained and you can proceed to remove the specimens and clean the retort Remove the specimens and drain the paraffin from the retort according to the...

Page 73: ...Chapter 3 Cleaning ...

Page 74: ...tion window appears Note The same applies when Clean Retort is selected on the Utility screen Caution If retort cleaning is not performed within 10 minutes after switching to this screen a window with the waning message Start Clean Retort appears Touching EXIT takes you back to the standby screen but if paraffin has been filled in the retort tissue processing warm water flush and automatic transfe...

Page 75: ...r flush use 3 to 4 liters of warm water whose temperature is 40 to 60 C for each station 5 When a station has been flushed but there is other station selected for flushing a message window appears Remove the reagent bottle that contained warm water from the flushed station put warm water in the bottle for the next station to be flushed and touch START to start flushing the next station 6 When all ...

Page 76: ...Chapter 4 Solution Exchange ...

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Page 78: ... the newly selected solution group blink and the stations that were blinking before return to steady illumination 4 Select a solution group and touch START 4 The Confirm window appears 5 Select whether to perform rinsing after automatic transfer 5 To perform rinsing select the Rinse C2 check box To not perform rinsing select the Don t Rinse check box When Rinse is selected the instrument will move...

Page 79: ...aged is shown 2 All Stations button Touching this button will select all stations Touching it again deselects the stations 3 Clear Usage Status button Touching this button clears the usage statuses of the station whose check field is selected 1 Clearing the usage data 1 Touch the check field corresponding to the name of the solution whose usage data you want to clear and appears If the set count h...

Page 80: ...Chapter 5 Program Creation Editing ...

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Page 82: ...olutions used for processing already exist and add create new solution names if necessary Editing Solution Configuration Details p 5 3 Set various items station color scope of stations solution group name usage management limits flush counts on the Edit Solution Configuration screen Editing Tissue Processing Program Details p 5 6 Select a program and enter a program name change the name Touch Edit...

Page 83: ...me 1 Storing a new solution name 1 Touch Add 4 2 Solution name entry screen appears Use the touch keyboard to enter a desired solution name up to 22 characters For how to use the touch keyboard refer to p 1 20 Take note that the new solution name cannot be stored if the same solution name already exists 3 Touch Save The screen is switched to the Edit Solution Names screen and the new solution name...

Page 84: ...e management method for C1 and C2 is displayed A desired usage management method can be set from among Days Runs and NONE by touching the display field 5 Export Data button Use this button to export the solution configuration 6 Save button Use this button to save the settings 1 Setting a solution group 1 Among the Solution group color icon buttons 1 touch the button of the color you want to set 2 ...

Page 85: ... the solution Solution usage is managed separately for Sta 1 to Sta 14 and for C1 and C2 When the settings are complete touch Save This will save the settings after which the Utility screen will be displayed Setting the management method for Sta 1 to Sta 14 Touch the Management Method 1 14 display area and select a desired method from the pop up menu If the management method is changed the values ...

Page 86: ...ems or instrument failures 5 Setting when to perform warm water flush If reagents such as buffered formalin are used precipitates are generated and may clog the tubing Accordingly warm water flush is performed regularly and when to perform warm water flush is managed Touch the setting area 1 in the Flush field displays a value entry window so enter a warm water flush frequency Touching Save saves ...

Page 87: ...ist The registered programs are displayed 3 Export Data button Use this button to export the program details 4 Program Name button Use this button to change the program name 5 Edit button Use this button to edit the program details 1 Changing a program name 1 Select from the program list 1 the program whose name you want to change 2 Touch Program Name 2 When the program name entry window appears e...

Page 88: ...precaution Caution Very volatile solutions evaporate easily when the pressure is reduced so avoid setting the P V to ON for these solutions Errors and tissue processing failures may occur 1 Program details display field Program details are displayed By touching the Sta or Solution Name field you can copy the settings of Time Temp P V Mix and Predicted Endtime for the selected station to all other ...

Page 89: ...nds are repeated OFF P V will not be performed Caution Very volatile solutions evaporate easily when the pressure is reduced so avoid setting the P V to ON for these solutions Errors and tissue processing failures may occur 6 Editing the program Mix In the program details display field touch Mix 1 for the station you want to edit and a window appears Select OFF Slow or Fast OFF Mixing will not be ...

Page 90: ...on number or solution name of the station whose settings you want to copy The Time Temp P V and Mix fields of the selected station will turn blue and a confirmation window will appear 2 Touch All Stations and the settings of the selected station will be copied to all other stations Note that the temperature setting will not be copied from a solution station to a paraffin station or from a paraffin...

Page 91: ...Chapter 6 Other Operations ...

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Page 93: ... Program field Name of the program used for the tissue processing End status field Result of tissue processing 2 Details button Touch this button to display the details of the processing result whose Check field is selected Touch the Check field of the tissue processing whose details you want to display to add a check mark Touching Details will display the Details window If two or more check marks...

Page 94: ...ting the program have been reflected If the experiment number setting is turned off on the System Setup screen is shown in the experiment number field If the solution management method is not set to Cassettes or it is set to Cassettes but the entered value is 0 on the Edit Solution Configuration screen is shown in the cassette count field 2 Run History button Use this button to show the operation ...

Page 95: ...ch on the right to display the next page Touch to move to the previous page Up to 100 errors are displayed starting from the latest one If 100 errors are already saved the oldest result will be deleted the next time a new error is added 1 Error log display field Error field The error number is shown Touching the error number displays the details of the error for 10 seconds Sta field Station number...

Page 96: ...time the date changes Current Usage field The status of usage is shown based on the Unit field Limit field The preset limit is shown 2 Clear button Touch and select the Current Usage field of the part whose usage data you want to clear and then touch Clear to clear the usage data Usage data can be cleared only for the retort seal and activated carbon filters If the replacement timing is passed app...

Page 97: ... Selecting ON lets you enter an experiment number at the start of tissue processing Experiment numbers cannot be entered if OFF is selected 4 Back Light Off button Use this button to set the time until the backlight turns off Touch the backlight display area and when the pop up menu is displayed select a desired option Options Always ON 15 minutes 30 minutes 45 minutes 1 hour 2 hours 3 hours and 4...

Page 98: ...esponding passwords and 20 user names and corresponding passwords Changing a user name Touch the user name you want to change and when the user name entry window appears enter a name Up to 22 characters can be entered Changing a password Touch the password field you want to change and when the password entry window appears enter a password A password can be set using one to six characters When the...

Page 99: ...r the sound test and the sound will be played Changing the volume You can set the volume to eight different levels by touching Volume 2 The initial value is 4 The volume increases by one level every time the button is touched When the button is touched at the eighth level the volume decreases to the first level Ending the sound setting Touch EXIT to return to the System Setup screen 4 Setting an i...

Page 100: ...om the current station 3 Changing station Touch Next Station 5 or Previous Station 6 A message window appears and the instrument starts moving through the stations Caution Be sure to drain the retort If the current station is Sta 11 12 13 14 or C1 and the rotary valve temperature is not heated to 62 C or more wait for the rotary valve temperature to rise If the current station is Sta 11 12 13 or 1...

Page 101: ...lect Language On the Utility tab screen touch the Select Language icon to open the Select Language window You can select one of the following nine languages English Japanese French German Spanish Portuguese Russian Korean Chinese ...

Page 102: ...Chapter 7 Maintenance and Inspection ...

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Page 104: ... the retort Every week Clean the exterior panels and top cover of the instrument Every week Clean the paraffin oven and reagent bottle spill tray Every month Replace the activated carbon filter Every month Replace the retort lid gasket Once every six months 2 Periodic Replacement In addition to the periodic maintenance performed by the user some of the key system and parts must be inspected and re...

Page 105: ... and dirt using a paraffin scraper etc Wipe dry using a cloth or paper towel 6 Return the spill tray in its original position Also return the paraffin container in its original position and close the paraffin oven door Caution If the paraffin deposits are not cleaned the door becomes unable to open 5 Cleaning of the Reagent bottle Rack and Spill Tray If the reagent bottle rack became dirty clean i...

Page 106: ...f detergent remains the tissue processing may be impaired When cleaning the reagent bottle do not use a brush or any other tool that may scratch the surface of the bottle The chemical resistant surface treatment of the reagent bottle may lose its intended effect 1 Removing and installing a bottle cap Removing the bottle cap Turn the bottle cap counterclockwise to remove the cap Installing the bott...

Page 107: ...affin container 3 Use a paper towel to wipe the interior and exterior of the paraffin container Use a paraffin scraper to clean if necessary 4 Return the paraffin container to its original position and close the door for the paraffin oven 9 Cleaning of the Retort Clean the retort as necessary Caution If buffered formalin is used white precipitates may deposit in the retort In this case clean the i...

Page 108: ...tort seal First check the installation direction The wider side should be facing the back of the groove in a side view of the retort seal 4 Determine the positions of a and b by making sure the right and left are even and then push the seal at a and b into the seal groove Push in the inner side first Next insert the seal at c and d into the groove by making sure the top and bottom are even and the...

Page 109: ...Chapter 8 Troubleshooting ...

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Page 111: ...cal representative Short circuited power input part Contact the Sakura Finetek Technical Support representative or local representative Short circuited switching power supply Contact the Sakura Finetek Technical Support representative or local representative Short circuited retort heater or transformer Contact the Sakura Finetek Technical Support representative or local representative Faulty circu...

Page 112: ...he Sakura Finetek Technical Support representative or local representative Faulty CPU board Contact the Sakura Finetek Technical Support representative or local representative Faulty LCD relay board Contact the Sakura Finetek Technical Support representative or local representative Faulty LCD Contact the Sakura Finetek Technical Support representative or local representative Sound is not output So...

Page 113: ...ssue processing program data is lost or corrupted Contact the Sakura Finetek Technical Support representative or local representative The CF card in the instrument is corrupted Contact the Sakura Finetek Technical Support representative or local representative Past error logs are no longer displayed Memory backup area in which error log data is stored is damaged or initialized Contact the Sakura F...

Page 114: ...tc Clean the retort seal side in the same manner Scratched or deformed retort seal causing leakage Check that there is no scratch etc on the retort seal If scratched replace the retort seal If no major scratch is found remove the retort seal once and then change the position and install it again If the fume still leaks replace the retort seal with a new one The retort lid tightening force is insuf...

Page 115: ...clearance When connecting the exhaust duct it is not connected correctly Confirm that the exhaust duct is firmly fitted into the duct connection adapter If the duct is loose tighten with a hose clamp etc When connecting an exhaust duct the duct connection path is too long Check the length of the exhaust duct The 38 duct will generate a large pressure loss if its length becomes 5 m or more in which...

Page 116: ...n scraper and then wipe off any remaining paraffin using xylene etc Paraffin hardened in the paraffin oven The ambient operating temperature is too low Paraffin sometimes solidifies when the operating temperature of the instrument drops to 10 C or below Do not install the instrument in a place where the operating temperature may drop to 10 C or below The preset processing temperature is too low fo...

Page 117: ... oven door gasket The paraffin oven door gasket is of the insertion type Accordingly opening the door when the gasket is stuck may cause the gasket to come off and prevent the door from closing In this case clean the gasket and insert it all the way into the groove Foreign matter on the catch receiving part Foreign matter may attach to the catches on each door or the catch contacting surfaces of t...

Page 118: ...ive USB flash drive is not recognized or the output cannot be made USB flash drive is not connected Make sure that the instrument recognizes USB flash drive certified as an option after USB flash drive is connected to the instrument and then the output button is pressed USB flash drive that is not accepted by the instrument was connected USB flash drive was connected in an incorrect timing USB fla...

Page 119: ... representative or local representative 005 Abnormal retort pressure level Faulty pressure sensor Contact the Sakura Finetek Technical Support representative or local representative Faulty CPU board Contact the Sakura Finetek Technical Support representative or local representative 010 Retort overflow More than the specified amount of tissue was processed If paraffin overflows however the trap cir...

Page 120: ...damaged Contact the Sakura Finetek Technical Support representative or local representative The trap bottle is loose or trap bottle cap is damaged Contact the Sakura Finetek Technical Support representative or local representative Damaged air tubing fitting or loose fitting Contact the Sakura Finetek Technical Support representative or local representative Faulty solenoid valve SV3 or SV2 Contact ...

Page 121: ...ffin precipitated salt Contact the Sakura Finetek Technical Support representative or local representative Clogged tubing in the air circuit Contact the Sakura Finetek Technical Support representative or local representative Detached reagent bottle coupler Do not remove the bottle once processing is started 031 Oven temperature sensor error Effect of environmental temperature Let the instrument st...

Page 122: ...d motor circuit or faulty motor unit Contact the Sakura Finetek Technical Support representative or local representative Faulty rotary valve position detection board Contact the Sakura Finetek Technical Support representative or local representative Faulty CPU board Contact the Sakura Finetek Technical Support representative or local representative 042 Retrying rotary valve operation Mis stepped m...

Page 123: ... representative or local representative 087 Rotary valve temperature exceeded 80 C Hot liquid was introduced into the instrument Do not put hot liquid Faulty CPU board Contact the Sakura Finetek Technical Support representative or local representative Faulty heater driver board Contact the Sakura Finetek Technical Support representative or local representative 088 Retort temperature exceeded 85 C ...

Page 124: ...local representative File in the CF card or USB flash drive is damaged file format error or write protected file Contact the Sakura Finetek Technical Support representative or local representative A specified CF card is not used Contact the Sakura Finetek Technical Support representative or local representative 200 USB flash drive not found Removed in the middle Or the USB flash drive failed Do no...

Page 125: ... Over 5 hours 12 hours and 30 minutes Dropped by no more than 18 C from the preset temperature 5 hours and 30 minutes Dropped by no more than 19 C from the preset temperature 5 hours and 35 minutes Dropped by more than 19 C from the preset temperature 12 hours and 30 minutes Stand by Times Required for Melting the Paraffin in the Retort Stand by Times after Drops in Retort Temperature Power Outage...

Page 126: ... years Retort seal 6 months Various boards 5 years LCD 5 years Power supply unit 5 years Various detection sensors 5 years Rotary valve 8 years Air pump excluding diaphragm 8 years Solenoid valve 8 years Pressure safety valve 8 years Heater 8 years The above service life of the instrument and service lives of key components in years are not guaranteed instead they are average periods calculated on...

Page 127: ...hen the instrument stops during tissue processing to protect the specimens being processed from becoming dry Experiment No Number assigned to identify each tissue processing P 2 7 Automatic Transfer Solution is automatically transferred to the subsequent solution stations one by one within the same solution group P 4 1 Endtime Date time the tissue processing ended P 6 3 Predicted Endtime Date time...

Page 128: ...e legally designated communicable diseases are treated Yes No 2 Has this device been used in a ward where patients of legally designated communicable diseases are staying Yes No 3 Has this device been used in a room where pathological work on infectious diseases is conducted Yes No 4 Has this device been used in a room where testing of infectious diseases is conducted Yes No 5 Has this device been...

Page 129: ...8 19 ...

Page 130: ...M01 018E 01 ...

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Sakura Histo-Tek VP1, Operating Manual, Page: 1 / 130

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Summary of Contents for Histo-Tek VP1

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