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MagNA Pure 96 System
Customer Training Guide, Version 1.0

Software Version 2.0

December 2010

Summary of Contents for MagNA Pure 96

Page 1: ...MagNA Pure 96 System Customer Training Guide Version 1 0 Software Version 2 0 December 2010...

Page 2: ......

Page 3: ...es 29 1 1 Preparing the Samples 29 1 2 Defining the Parameters in the Software 32 1 3 Loading the Stage 34 2 Setting up a Run for Purification of Cellular RNA from Cultured Cells 43 2 1 Preparing the...

Page 4: ...MagNA Pure 96 System Customer Training Guide V1 0 Table of Contents 4...

Page 5: ...bH Roche Applied Science Customer Support Nonnenwald 2 82372 Penzberg Germany Every effort has been made to ensure that all the information contained in the MagNA Pure 96 Customer Training Guide is co...

Page 6: ...not for use in diagnostic pro cedures The system was neither developed nor validated by the manufacturer for any kind of in vitro diagnostic application Any use of the MagNA Pure 96 System as a front...

Page 7: ...or could cause damage to the instrument HOT SURFACE This symbol is used to label potentially hot instrument surfaces BIO HAZARD This symbol is used to indicate that certain precautions must be taken w...

Page 8: ...r the MagNA Pure 96 Instrument for use by all op erators Symbol Heading Description MANUFACTURER OF DEVICE On the instrument type plate CE MARK The CE mark on the instrument type plate indicates confo...

Page 9: ...to disassemble the equipment Repairs must be performed by Roche service personnel The equipment contains no operator serviceable parts Do not modify any part of the equipment Modification may cause fi...

Page 10: ...ct Please follow the instructions of the respective manufacturer for more information on decontamination agents see section Operator Maintenance of the MagNA Pure 96 Operator s Guide The MagNA Pure 96...

Page 11: ...strument Open the load flap Ensure that The internal or external system fluid container is not empty If it is empty see sections To replace the internal system fluid container or To replace the extern...

Page 12: ...ialization the main window with the Overview tab is displayed Click LogOn in the global action bar The LogOn dialog box opens Enter your User ID and Password User ID Admin Initial Password MagNAPure96...

Page 13: ...change the system fluid container see section System fluid Check visually if there is enough space in the waste container If necessary change the waste container see section Waste containers To prime...

Page 14: ...re in the MagNA Pure 96 Operator s Guide In the MagNA Pure 96 Software select the Instrument tab In the Instrument tab select the Maintenance Service sub tab In the tree view select Daily Maintenance...

Page 15: ...rument tab select the Maintenance Service sub tab In the tree view select Daily Maintenance The substructure below Daily Maintenance is expanded Select the check box in front of Confirm Daily Maintena...

Page 16: ...he MagNA Pure 96 Instrument checks if there is enough system fluid left for a purification run of 96 samples If there is not enough the status of the system fluid is indicated as inadequate in the ins...

Page 17: ...b on the aspiration port and extract the tube Place the tube in the holder in the top left corner of the compartment frame Remove the aspiration port from the empty container To prevent contamination...

Page 18: ...er completely into its compartment In the MagNA Pure 96 Software switch to the Workplace tab and then to the Stage sub tab In the instrument status area click Replace The Replace System Fluid dialog b...

Page 19: ...n port from the empty container and insert it into the new container To prevent contamination of the aspiration port transfer it immediately into the new system fluid container In the MagNA Pure 96 So...

Page 20: ...city status of the waste container is indicated as inadequate in the instrument status area of the Stage sub tab in the MagNA Pure 96 Software In this case drain the waste container To drain the inter...

Page 21: ...ccording to local regulations Rotate the internal waste container during draining and slowly pour out all liquid Keep the filter of the vent hole dry to avoid spilling of liquid waste through the vent...

Page 22: ...b and then insert the waste tube When you hear a click the tube is fixed Ensure that the sensor and the outside of the container are dry Ensure that the waste tube is properly connected to the waste c...

Page 23: ...Start up Preparing the MagNA Pure System 23 A A Waste containers Remove the black adapter from the external waste container Remove the silver fastener from the external waste container...

Page 24: ...0 24 Preparing the MagNA Pure System A A Waste containers Close the external waste container with its lid Transfer the waste container horizontally to prevent liquid spillage Remove both caps the one...

Page 25: ...eagent see chapter Mainte nance and Care in the MagNA Pure 96 Operator s Guide Clean the waste container with water and dry it outside before reinstalling the adapter and the sensor Reinstall the adap...

Page 26: ...MagNA Pure 96 System Customer Training Guide V1 0 A A 26...

Page 27: ...med for 96 samples and the default MagNA Pure 96 Plates are used The MagNA Pure 96 Processing Cartridge as source plate The MagNA Pure 96 Output Plate as target plate Preconditions for all runs A run...

Page 28: ...MagNA Pure 96 System Customer Training Guide V1 0 28 B B...

Page 29: ...e section Defining the Parameters in the Software 3 Loading the instrument see section Loading the Stage 1 1 Preparing the Samples Always use sterile nuclease free pipettes To prepare the internal con...

Page 30: ...new internal control if you wish Click in the Protocols list and select the DNA Blood SV protocol to which the internal control is applied In the Test Volume list select the internal control volume p...

Page 31: ...lick OK to save your settings The Edit Internal Control Barcode dialog box closes Click OK to save your settings The Settings dialog box closes Ensure that there are no clots in the anticoagulated who...

Page 32: ...les Once it has this information the software calculates the type and amount of isolation reagents and dis posables To create the order In the Overview tab click The Orders sub tab opens Above the Tes...

Page 33: ...le names from scratch Scan the barcodes of sample tubes with the handheld barcode scanner Load an existing sample template and modify the sample data if necessary In the local tool bar the button is e...

Page 34: ...ulation steps in side the MagNA Pure 96 Instrument and all manual pipetting steps When removing the racks from the instrument the outline color of the individual racks on the Stage sub tab changes to...

Page 35: ...in the same way Ensure that the clips fix the bottle rims completely and that the barcode labels of all bottles face left so that they can be read by the barcode reader during the resource check Espec...

Page 36: ...mber and label of the reagent trays Position of the reagent trays in the reagent racks Processing cartridge The bottom left frame has a rectangular cut that fits onto an elevation in the relevant rack...

Page 37: ...as recommended on the Stage sub tab Dispose of damaged reagent trays e g if a reagent container has fallen out or is missing Only bottles and trays of the same kit lot can be used in the same purific...

Page 38: ...isplayed in blue on the Stage sub tab Pull the tip rack all the way out of the stage Place three tip trays in the tip rack as recommended on the Stage sub tab of the MagNA Pure 96 Soft ware Ensure tha...

Page 39: ...agNA Pure 96 Output Plate in position 4 of the purification rack Place the processing cartridge with the pre pipetted samples in position 1 of the purification rack Ensure that the barcode labels of b...

Page 40: ...re 96 Instrument detects that an accessory or disposable is missing invalid or in the wrong position a message is displayed For more information on the resource check see chapter Software in the MagNA...

Page 41: ...ware looks as follows Figure 1 Stage sub tab after a Successful Resource Check Proceed with Performing a Run Yellow The accessory or disposable is missing Red The accessory or disposable is invalid or...

Page 42: ...MagNA Pure 96 System Customer Training Guide V1 0 B B 42...

Page 43: ...tration of trypsin Always use sterile nuclease free pipettes To prepare adherent cells Visually check the source plate to ensure that the samples are pipetted into each individual well of the source p...

Page 44: ...of the cell culture incubator Shake the bottle slightly to resuspend the cells completely Transfer the cell suspension into a suitable centrifugation tube Centrifuge the cells for 10 minutes at 300 x...

Page 45: ...le list In the Volume group select 100 in the Elution list Select MP96 Output Plate from the Target Plate list Specify the sample data in the sample table with the names and positions of all samples p...

Page 46: ...all manual pipetting steps When removing the racks from the instrument the outline color of the individual racks on the Stage sub tab changes to yellow indicating that the racks are not present When y...

Page 47: ...y can be read by the barcode reader during the resource check Especially if you use the same MGP bottles during subsequent runs the bottles can rotate during the shaking process so that the barcode ma...

Page 48: ...e re source check You do not need to remove a partially used tip tray from the tip rack It is a common scenario to load the tip rack with full tip trays and partially used ones from former runs Insert...

Page 49: ...k see chapter Software in the MagNA Pure 96 Operator s Guide The error message is displayed in the corresponding graph on the Stage sub tab of the MagNA Pure 96 Software The graph on the Stage sub tab...

Page 50: ...he rearranged rack Once the resource check is successfully completed the Stage sub tab of the MagNA Pure 96 Software looks as follows Figure 2 Stage sub tab after a Successful Resource Check Proceed w...

Page 51: ...pty If during the load check the MagNA Pure 96 Instrument detects a lack of waste capacity a message is dis played For more information on the load check see chapter Software in the MagNA Pure 96 Oper...

Page 52: ...amples processed Depending on the protocol and sample material selected the duration of a purification run can vary DNA and Viral NA Small Volume Kit about 50 minutes DNA and Viral NA Large Volume Kit...

Page 53: ...a Run Open the load flap The Overview tab then indicates the used accessories and disposables Click the button The results are displayed on the Results sub tab of the MagNA Pure 96 Soft ware see chapt...

Page 54: ...output plate The cooling plate is not put back under the output plate which means the eluates will no longer be cooled Therefore remove the Output Plate from the stage and store the eluates on ice or...

Page 55: ...After thawing eluates mix them gently by pipetting up and down ten times before performing any downstream steps e g PCR RT PCR or OD measurements The mixing volume should be at least half of the eluat...

Page 56: ...eeded allowed time after first use Exceeded on board time Exceed number of re uses Contain residual reagents Are damaged e g if a reagent container has fallen out or is missing To unload the bottle ra...

Page 57: ...rile side of the backing paper of the sealing foil run the blue lid of the reagent tray or alternatively the flat part of your hand over the top of the reagent trays Handle the sealing foil carefully...

Page 58: ...w hours depending on the decontamina tion cleaning reagents and procedures Discard the tip trays with the wasted tips according to local regulations Insert the waste rack back into the stage Open the...

Page 59: ...to ensure exact pipetting results When calculating the volumes for the volume transfer function the MagNA Pure 96 Software considers the dead volume Consequently there will be some residual liquid le...

Page 60: ...late ID field The plate type in the Source Plate field The volume present in the Volume Source field The names of the eluates in the sample table In the Test Parameter area select a PCR Setup protocol...

Page 61: ...Instrument and all manual pipetting steps When removing the racks from the instrument the outline color of the individual racks on the Stage sub tab changes to yellow indicating that the racks are not...

Page 62: ...Place the plate holder in position 3 of the purification rack Ensure that position A1 of the LightCycler 480 Multiwell Plate 96 is positioned in the top left cor ner Insert the purification rack back...

Page 63: ...the barcode label faces left so that it can be read by the barcode reader during the resource check You do not need to remove a partially used tip tray from the tip rack It is a common scenario to lo...

Page 64: ...6 Operator s Guide The error message is displayed in the corresponding graph on the Stage sub tab of the MagNA Pure 96 Software The graph on the Stage sub tab is color coded After the resource check t...

Page 65: ...ompleted the Stage sub tab of the MagNA Pure 96 Software looks as follows Figure 5 Stage Sub tab after a Successful Resource Check During the load check the MagNA Pure 96 Instrument requests a visual...

Page 66: ...MagNA Pure 96 System Customer Training Guide V1 0 B B 66...

Page 67: ...trument To shut down the software To switch off the instrument Ensure that all necessary data is saved In the global action bar select the button If there is an unsaved order present a dialog box prom...

Page 68: ...MagNA Pure 96 System Customer Training Guide V1 0 C C 68...

Page 69: ...External system fluid 19 waste container 22 F Fresh blood samples 31 Frozen blood samples 31 G Genomic DNA 29 I Initial password 12 administrator 12 Internal system fluid 16 waste container 20 Intern...

Page 70: ...ce check 40 49 64 start 51 S Samples adherent cells 43 fresh blood 31 frozen blood 31 suspension cells 44 Setup DNase solution 44 Setup run cellular RNA from cultured cells 43 genomic DNA of whole blo...

Page 71: ......

Page 72: ...Published by Roche Diagnostics GmbH Sandhofer Stra e 116 68305 Mannheim Germany 2010 Roche Diagnostics All rights reserved www roche applied science com MP96_CustoTrainGuide indd 2 29 11 2010 14 53 47...

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