Section 8
Saturn 5™ Laser Operation
8
34
Research Instruments Ltd
How to Check Laser Target Alignment for Saturn 5 Fixed Laser System
1.
Click the
Laser Control
icon.
2.
Click
Laser Settings.
3. Click
Align Laser.
4.
Ensure embryos have been removed from the field of view.
5.
Using an empty Petri dish or a slide adjust the focus of the pilot laser to ensure the pilot laser
spot is as small as possible. The brightness of the pilot laser can be adjusted.
6.
Click the centre of the pilot laser.
7.
Click
Finish.
How to Check Hole Size Calibration
The laser objective must be calibrated correctly for the Hole Size Indicator to work correctly.
The actual size of hole a laser produces may vary depending on the type of Petri dish and media, the
characteristics of the embryo and other factors. For this reason, the Hole Size Indicator may be calibrated.
To calibrate the Hole Size Indicator, you must be using the red laser objective and select the 40X or
20X objective in RI Viewer depending which magnification laser objective is installed. A suitable test
specimen (which will be drilled) should be in the laser target area.
2. Click the
Hole Size Calibration
button.
3. Put the laser target on top of the specimen.
4. Fire the laser and ablate an area of the sample specimen.
5. Click the up and down arrows next to Adjust Hole Size to match to the size of the drilled hole.
1.
Click the
Laser Settings
button on the Laser Control panel
