QIAsymphony SP/AS Consolidated Operating Guide 12/2017
177
13.7.3
Errors occurring during an assay run
Problems with labware or with liquid spills
Error
Comments and suggestions
Wrong reagent tube
placed in adapter.
Problems with tip positioning could occur. This may result in pipetting of incorrect volumes and
problems during the assay run and data analysis. In addition, the tip might crash into the
tube/plate and result in damage to the QIAsymphony AS.
Positioning in xy direction
changes.
Ensure that the workbench that the QIAsymphony SP/AS instruments are placed on meets the
requirements (see Section 4.2 of the
QIAsymphony SP/AS User Manual — General
Description
). We recommend using the QIAsymphony Cabinet SP/AS for correct positioning of
the QIAsymphony SP/AS instruments.
Ensure that the drawers are properly closed.
Liquids in adapter.
Ensure that all consumables are placed in the correct positions on the worktable. The inventory
scan does not check whether the correct tubes/plates are placed in the corresponding adapters.
Condensation on the
worktable.
Depending on the environment in the laboratory, it is possible that condensation forms on the
worktable. Wipe away condensation according to the daily maintenance procedures, see
Section 9.2 of the
QIAsymphony SP/AS User Manual — General Description
.
Filter-tips are bent or
deformed after liquid
transfer.
Ensure that the correct rack type is defined on the correct slot.
Ensure that the rack is correctly positioned on the adapter.
Only use rack types that are compatible with the defined adapter.
Assay run interruption
Error
Comments and suggestions
Inventory status during run
not correct.
Ensure that an inventory scan is performed on each drawer if something on the worktable has
been changed.
The protocol was
interrupted or stopped by
the system due to an
error.
Remove consumables from the worktable. If necessary, see Section 2.13 of the
QIAsymphony
SP/AS User Manual — Operating the QIAsymphony AS
for details about protocol recovery and
manually completing assay setup.
The length of time that a protocol can be stopped/interrupted may be defined in some
protocols. Any stop, pause, or interruption of a protocol will lead to samples being flagged as
“unclear”.
Missing tips.
If insufficient tips are available, reload tips onto the worktable.
If sufficient tips are available and the error persists, change the tip rack position. It is possible
that the pipetting head cannot reach the tips.
Not enough liquid found. Ensure that the correct volume is provided and that the plates/tubes and adapters as defined in
the assay definition are provided.
Ensure that there are no air bubbles on the surface of the liquid.
Add more liquid.
Summary of Contents for QIAsymphony SP
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