Qiagen QIAsymphony SP, Operating Manual

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QIAsymphony SP/AS Consolidated Operating Guide 12/2017 143
Important
It is not possible to modify assay parameters in work list mode.
Important
If the parameters are modified, the changes will not be saved in the Assay
Parameter Set. They will be used for the current run only. To change parameters
in an Assay Parameter Set for future runs, use the
Process Definition editor tool of
the QIAsymphony Management Console. For further information, refer to the
QIAsymphony Management Console User Manual .
12.4.6 Queuing an independent assay run
When assay definition is completed the assay run can be queued. Proceed as follows:
1. Press Queue in the Assay Assignment screen.
The QIAsymphony SP/AS instruments now validate the assay run and
create a loading information file.
Once an assay run is queued it is not possible to return to the assay
definition process
2. The Loading Information screen appears.
You can now load the instrument worktable. Refer to Section 12.4.1 for
more information
12.4.7 Validating the assay run
QIAsymphony SP/AS instruments validate all defined values for the assay run and determine
whether the assay run can be loaded. The validation process includes the following checks:

Checks that the number of assay positions required does not exceed the number of positions
available on the assay rack(s), according to the defined Assay Parameter Set(s) (internal
software check)

Checks that the total volume of master mix required does not exceed the available volume in
the largest master mix bottle (internal software check)

For sample positions that need normalization, checks that the dilution parameters are in
specified range
If anything is incorrect, an error message will appear informing the user about exactly what is
incorrect. The run cannot be loaded until the message is acknowledged and the problem is
corrected.