Qiagen QIAgility, User Manual, Page: 248 / 334

Instrument Setup and Calibration
7-14 QIAgility User Manual 11/2011
3. Select the correct plate function, from the drop-down
menu, of the plate in which tip offset calibration will be
performed.
4. Click on the button. The “Plate/well Selector” screen
will open. Left-click on a plate to select the location of
the plate in which tip offset calibration will be performed.
5. Ensure that the correct tip type is selected in the “Select
tip types to profile” panel.
6. Define the number of tips to be used to calculate the
average offset.
7. Click on the “Start” button to start tip offset calibration.
7.1.6 Calibrate volumes
There is a direct relationship between the rotation of the
motor shaft and the vertical movement of the pipet piston.
Volume calibration defines the number of units that the P-
axis motor must move to accurately deliver a given volume of
liquid.
Volume calibration is performed using the “Volume
Calibration Wizard”. During installation of the QIAgility, the
user should perform “Option 1” from the “Volume
Calibration Wizard”.
Note : Volume calibration must be done before the start of
the first run on a newly-installed QIAgility. An error is shown
at the start of the run if no volume calibration has been
done.