Qiagen QIAgility User Manual Download Page 188

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QIAgility Software

5-126

QIAgility User Manual 11/2011

5.9.8

Progress report

When a run is in progress, the status of the run is

summarized in the right-hand pane.

5.10

Programming run files

5.10.1

PCR Setup Wizard

The “PCR Setup Wizard” enables typical PCR reactions, with

samples, standards, and master mixes, to be set up. The

wizard allows the user to set up reagents, and then use these

to prepare master mixes. The wizard can also be used to set

up master mixes without setting up reactions.
This section outlines the “PCR Setup Wizard” screens that will

guide the user through the setup. Instructions are displayed

on each screen.

Summary of Contents for QIAgility

Page 1: ...Technical 0800 914413 France n Orders 01 60 920 926 n Fax 01 60 920 925 n Technical 01 60 920 930 n Offers 01 60 920 928 Germany n Orders 02103 29 12000 n Fax 02103 29 22000 n Technical 02103 29 12400...

Page 2: ...echnical 0800 914413 France n Orders 01 60 920 926 n Fax 01 60 920 925 n Technical 01 60 920 930 n Offers 01 60 920 928 Germany n Orders 02103 29 12000 n Fax 02103 29 22000 n Technical 02103 29 12400...

Page 3: ...e safety 1 7 1 8 Symbols on the QIAgility 1 8 2 Introduction 2 1 2 1 About this user manual 2 1 2 2 General information 2 2 2 2 1 Technical assistance 2 2 2 2 2 Policy statement 2 3 2 2 3 Version mana...

Page 4: ...2 4 4 Unpacking the QIAgility 4 3 4 5 Installing the QIAgility 4 4 4 5 1 UV lamp 4 6 4 5 2 HEPA filter system 4 6 4 5 3 Instrument calibration 4 6 4 5 4 Computer setup and installation of software 4...

Page 5: ...om setup disk 5 27 5 5 3 Delete multiple calibrations 5 28 5 6 Configure run settings 5 29 5 6 1 Configure tip air intake 5 30 5 6 2 Configure pipet speed 5 30 5 6 3 Set master mix extra volume 5 32 5...

Page 6: ...126 5 10 2 Optimisation Wizard 5 135 5 10 3 Using Empty Project 5 141 5 10 4 Example reactions 5 142 5 10 5 Checking the reaction setup 5 145 5 10 6 Required volumes for reaction setup 5 145 5 11 Sel...

Page 7: ...eights 7 7 7 1 4 Optimal height calibration 7 11 7 1 5 Calibrate tip offsets 7 11 7 1 6 Calibrate volumes 7 14 8 Maintenance Procedures 8 1 8 1 Regular maintenance 8 2 8 1 1 Cleaning the QIAgility 8 2...

Page 8: ...onformity A 5 Appendix B B 1 Liability clause B 1 Appendix C C 1 Safety Information French FR C 1 1 Consignes de s curit C 1 1 1 Utilisation appropri e C 2 1 2 S curit lectrique C 4 1 3 S curit biolog...

Page 9: ...1 Proben D 5 1 4 Chemikalien D 7 1 4 1 Giftige D mpfe D 7 1 5 Abfallentsorgung D 7 1 6 Gefahren durch mechanische Teile D 8 1 7 Sicherheitshinweise Wartungsarbeiten D 9 1 8 Symbole auf dem QIAgility D...

Page 10: ...Contents Contents 8 QIAgility User Manual 11 2011 This page intentionally left blank...

Page 11: ...situations that could result in personal injury to you or other persons Details about these circumstances are given in a box like this one CAUTION The term CAUTION is used to inform you about situatio...

Page 12: ...al injury and material damage W4 Do not attempt to move the QIAgility during operation WARNING CAUTION Explosive atmosphere W5 The QIAgility is not designed for use in an explosive atmosphere WARNING...

Page 13: ...al is likely to make the instrument dangerous Intentional interruption is prohibited Lethal voltages inside the instrument When the instrument is connected to line power terminals may be live and open...

Page 14: ...les may contain infectious agents You should be aware of the health hazard presented by such agents and should use store and dispose of such samples according to the required safety regulations WARNIN...

Page 15: ...all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration United States of America ACGIH American Conference of Government Industrial Hygi...

Page 16: ...on of the QIAgility Only use recommended tips and tip racks Keep the instrument lid closed whenever possible Do not disable the lid interlock When calibrating plates with the lid open always stand cle...

Page 17: ...on the QIAgility Risk of personal injury and material damage Only perform maintenance that is specifically described in this user manual CAUTION Damage to the instrument C4 Do not use solvents or rea...

Page 18: ...te on the back of the instrument CE mark for European Conformity Type plate on the back of the instrument CSA listing mark for Canada and the USA Type plate on the back of the instrument Legal manufac...

Page 19: ...11 1 9 Symbol Location Description Type plate on the back of the instrument RoHS mark for China the restriction of the use of certain hazardous substances in electrical and electronic equipment Back o...

Page 20: ...Safety Information 1 10 QIAgility User Manual 11 2011 This page intentionally left blank...

Page 21: ...in a safe condition 2 1 About this user manual This user manual provides information about the QIAgility in the following sections 1 Safety Information 2 Introduction 3 General Description 4 Installa...

Page 22: ...erienced scientists with extensive practical and theoretical expertise in sample and assay technologies and the use of QIAGEN products If you have any questions or experience any difficulties regardin...

Page 23: ...l version 3 0 2 3 Intended use of the QIAgility The QIAgility is designed to perform automated PCR setup and liquid handling actions in molecular biology applications If used in combination with QIAGE...

Page 24: ...requirements No special requirements Installation Laboratory technicians or equivalent Appropriately trained and experienced personnel familiar with use of computers and automation in general Routine...

Page 25: ...ductive tips enables high precision pipetting 1 The QIAgility worktable is set up with a Master Mix Block a Reagent Block tips and plates tubes The instrument is then calibrated Note Tubes are placed...

Page 26: ...lity User Manual 11 2011 3 2 The QIAgility Lid Pipetting head Robotic arm Feet Tip Rack Holder and tips Tip chute Tip Rack Holder and tips HEPA filter Master Mix Block and Reagent Block UV lamp 1 2 5...

Page 27: ...robotic arm to pause Extreme care should always be taken when opening the instrument lid during a run as the pause in robotic operation may take several seconds to be completed The instrument lid may...

Page 28: ...gility the tip chute is held in place by a locating block allowing easy placement and removal for cleaning Note Details on removing and replacing the tip chute tip ejector chute are described in Secti...

Page 29: ...trument lid is securely closed For increased safety an electronic interlock ensures that the UV lamp operates only when the instrument lid is closed Under no circumstances should the interlock be disa...

Page 30: ...uld always be fixed or locked before the QIAgility is moved 3 2 7 Worktable The worktable is the instrument surface upon which items such as plates reagents and tips are placed Worktable layout is spe...

Page 31: ...ser Manual 11 2011 3 7 3 2 9 Accessory panel QIAgility accessory panel Accessory 1 USB socket Accessory 2 Accessory power outlet Accessory 3 Main power input socket Serial RS 232 socket Power switch 1...

Page 32: ...0 Internal features of the QIAgility X axis Worktable Y axis Master Mix Block Z axis Reagent Block Pipetting head cover Tip Rack Holders and tips Pipetting head Y arm Pipetting hub Tip ejector 1 2 3 4...

Page 33: ...head is located under the protective pipetting head cover The pipetting head consists of a motor driven backlash compensated pipetting mechanism This mechanism is similar to most hand pipets Y arm Th...

Page 34: ...The QIAgility is supplied with 2 Master Mix Blocks which can hold 5 x 5 ml free standing tubes 1 x 5 ml free standing tubes and 4 x 1 5 ml tapered tubes Reagent Block The Reagent Block is intended to...

Page 35: ...Tubes 1 x Adapter Flip Cap 32 x 1 5 ml Tapered Base The 96 well adapters are intended to hold strip tubes and unskirted and half skirted PCR plates The 32 well adapter enables caps to be locked in pos...

Page 36: ...eactions and are normally supported by the 96 well adapters Important Tubes supplied by different manufacturers may have slightly different dimensions despite a similar appearance Such differences can...

Page 37: ...e liquid inside a tube the smaller the volume of liquid inside the tube the lower the change in capacitance For correct functioning of the liquid level sensor it is essential that the plates that are...

Page 38: ...is supplied as part of the QIAgility System However if a different computer is used to operate the QIAgility or to run the QIAgility Software then the following desktop or laptop hosting systems are...

Page 39: ...ty PCMCIA computer converter RS 232 QIAgility Note The laptop that is supplied with the QIAgility supports RS 232 and USB connections We recommend that the QIAgility be connected to the laptop via the...

Page 40: ...General Description 3 16 QIAgility User Manual 11 2011 This page intentionally left blank...

Page 41: ...arefully A change in position necessitates a recalibration Place the QIAgility in its final position The requirements for the QIAgility location and position are given below The QIAgility must be loca...

Page 42: ...that the QIAgility does not share a wall socket with other laboratory equipment and that the QIAgility is plugged directly into its own wall socket Important The wall socket should be easily accessibl...

Page 43: ...er outlet that has no ground earth connection 4 4 Unpacking the QIAgility Before unpacking the QIAgility move the package to the site of installation and check that the arrows on the package point upw...

Page 44: ...ng it Retain the packaging in case you need to transport or ship the QIAgility in the future Instructions for packing the QIAgility are outlined in Appendix H Using the original package minimizes dama...

Page 45: ...dures QIAgility User Manual 11 2011 4 5 2 Move the pipetting head to the right and remove the transport lock from the worktable 3 Unscrew the Z axis locking screw When this screw is removed the pipett...

Page 46: ...fixing into the UV plug Always wear safety gloves WARNING UV irradiation hazard W9 An electronic interlock ensures that the instrument lid must be closed for operation of the UV lamp Under no circumst...

Page 47: ...y Software See Section 4 6 for detailed information about how to install the software Note Some virus protection software is known to interfere with QIAgility Software and will cause the software to r...

Page 48: ...ill guide you through the installation Instructions are displayed on each screen of the wizard Note We recommend using the default settings for all screens 7 Click on the Next button to continue with...

Page 49: ...Note If a previous software version is already installed the Select Destination Location screen will not appear The following message box will appear instead 10 To uninstall a previous software versio...

Page 50: ...menu to select your region Click on the Next button to continue 12 Select the additional tasks and then click on the Next button Select Create a desktop icon to create a desktop icon to easily launch...

Page 51: ...anual 11 2011 4 11 13 Review the displayed details Click on the Install button 14 The QIAgility Software will now be installed onto your computer This could take a few minutes 15 If you are using Wind...

Page 52: ...y User Manual 11 2011 For correct installation of USB drivers click on the Continue Anyway button 16 If you are using Windows 7 the following message will appear For correct installation of USB driver...

Page 53: ...n click on the Next button to continue with the software installation 18 To directly launch the QIAgility Software select Launch QIAgility and then click on the Finish button If you do not want to lau...

Page 54: ...el The Control Panel window will open 2 Double click on the Add or Remove Programs icon 3 Select QIAgility from the list of installed programs and then click on the Remove button To uninstall the soft...

Page 55: ...re saved in this subfolder Data SupportPack ages Diagnostic support packages are saved in this folder Help Contains a PDF of the QIAgility User Manual version 3 0 HTML Contains a HTML version of the r...

Page 56: ...empt to modify or delete these folders 4 7 1 QProtocols folder Storing Q Protocols in this folder enables access to the Protocols tab in the splash screen that opens when the software starts and also...

Page 57: ...ake both connections at the same time To connect via the RS 232 interface Connect the 9 pin serial cable between the RS 232 port on the instrument and the RS 232 port on computer To connect via the US...

Page 58: ...led on the computer before the QIAgility is switched on see Section 4 5 4 Connect the QIAgility to the power outlet as follows 1 Ensure that the power switch of the QIAgility is set to the off positio...

Page 59: ...ty User Manual 11 2011 4 19 1 Select No not this time and then click on the Next button 2 We recommend that you select Install the software automatically 3 Click on the Next button to continue 4 Click...

Page 60: ...d 2 Set the power switch of the QIAgility to the on position and switch the computer on The pipetting head should move upwards If this does not happen please consult the Troubleshooting section of thi...

Page 61: ...click Empty Project last used layout or Empty Project default layout 6 The following message appears 7 Click on the OK button to proceed to tip ejector calibration See Section 7 1 1 for more details...

Page 62: ...Installation Procedures 4 22 QIAgility User Manual 11 2011 This page intentionally left blank...

Page 63: ...Protocols can be downloaded from www qiagen com goto QIAgility Select the User Support tab and look under the Protocols header For convenience the software automatically calculates the required volum...

Page 64: ...g operations on the instrument This is particularly useful for checking new run files In Virtual Mode all software functions are enabled Run files which are developed in Virtual Mode can be saved and...

Page 65: ...or Software workspace Menu bar Mode indicator Toolbar Software workspace 5 3 1 Menu bar The menu bar contains 5 menus File Control Wizards Options and Help All software functions can be controlled fro...

Page 66: ...options to create a new run file open a recently used run file or to open a Q Protocol Open Enables an existing run file to be opened Select the run file and then click on the Open button This file c...

Page 67: ...protocol Unlocks the protocol which is currently loaded This option is only available when a Q Protocol or User Developed Protocol is loaded 1 8 Lists the 8 run files which have been used most recentl...

Page 68: ...ction data or generate pre run reports PCR setup wizard Launches the PCR Setup Wizard This wizard can be used to create new run files See Section 5 10 1 for further details Optimisation wizard Launche...

Page 69: ...Options menu The Options menu includes options to manage user groups manage calibration settings and to configure pipetting and plate options User management Provides options to create user groups an...

Page 70: ...ange of storage devices e g USB stick See Section 5 5 1 Calibration management Load calibrations from setup disk Enables calibration settings to be loaded from a setup disk onto a computer See Section...

Page 71: ...Enables selection of the COM port which is used for communication with the QIAgility See Section 5 11 Robot setup Enable UV light In Virtual Mode this option enables or disables a virtual UV lamp Whe...

Page 72: ...ment of the pipetting speed i e the aspirate eject speed See Section 5 6 2 Run settings Use liquid level when pipetting Enables the method of tracking liquid level to be selected There are 3 options D...

Page 73: ...clude calibration settings Run settings reset settings to factory default Resets software settings to the factory defaults Click on the Yes button to continue Run settings System colour configuration...

Page 74: ...e See Section 5 7 1 Change accessory Launches the Select Accessory window which enables the user to add or remove an accessory from a selected position on the software worktable See Section 5 7 1 Chan...

Page 75: ...formed since the last instrument service Explore save directory Launches a file Explorer window with the default file directory where run files are saved Display log file Displays the log file This fi...

Page 76: ...ameters except tip availability are saved Select this option to start a run See Section 6 4 5 Pauses the instrument during a run A dialog box will appear Click on the Continue Run button to continue t...

Page 77: ...HEPA filter is not installed this icon will be shown in gray See Section 6 3 Indicates a problem with the run Errors must be resolved and acknowledged before the run can continue Opens the Service Re...

Page 78: ...uid level sensing option Tip reuse option User logged in Windows Plate buttons Right hand pane Software worktable The software worktable visually simulates the instrument worktable The 6 SBS positions...

Page 79: ...indicates whether the liquid level sensor is enabled Double click on this bar to change between Tip sensor Estimate and Not Used Tip sensor Indicates that liquid level sensing is enabled Estimate Indi...

Page 80: ...osition or height calibrated and will remain visible until all of the calibrations for that plate have been completed Warning Prompts the user to proceed to plate calibration Opens the Set Alignment d...

Page 81: ...he right hand pane when Minimize or Restore has been selected from the same icon in the menu bar Left click on the icon to access the following options Restore Reduces the size of the right hand pane...

Page 82: ...formation about the Reaction right hand pane Progress report Please refer to Section 5 9 8 for more detailed information about the Progress report right hand pane 5 4 User management User management a...

Page 83: ...st If required standard Windows file system write protection mechanisms may be used to protect run file data 5 4 2 User classes The QIAgility Software supports the following user groups QIAgility Oper...

Page 84: ...rs can Enable and disable the access control function in the software Enable User Access option see Section 5 4 3 page 5 23 Update group membership for each of the QIAgility groups if the Manager is a...

Page 85: ...access control when this option is enabled If the access control is enabled it is directly active Be careful to enable the access control only if you are a Manager 5 4 4 Managing user groups If your...

Page 86: ...011 4 Select the group to which the selected user should be assigned i e Administrators QIAgility Manager QIAgility Designer or QIAgility Operator in the Windows Groups panel 5 Click the button to mov...

Page 87: ...or a pop up of the operating system will appear to ask for elevation 5 4 5 Change current user To change users the current user must shut down the software and log out of Windows The new user must sub...

Page 88: ...re settings from one computer to another using a Setup Disk This is useful when the instrument is connected to a new computer The Setup Disk can be any storage device e g USB stick Note It is not nece...

Page 89: ...utton to continue 3 A default filename and location will be supplied If desired these can be changed 4 Click on the Save button The current calibration settings are now saved on the Setup Disk and can...

Page 90: ...trument has existing calibration settings the following dialog box will appear 4 Click on the Yes button to overwrite the existing calibration settings 5 5 3 Delete multiple calibrations To delete exi...

Page 91: ...The default run settings are the recommended settings However in some circumstances it is useful to adjust the run settings The default run settings are as follows Level Sensor On Error Mode Interrup...

Page 92: ...eactions Alternatively to return to the default air volume settings click on the Restore Defaults button Note Larger air volumes may be beneficial when pipetting viscous liquids Smaller air volumes ma...

Page 93: ...normal Defines the aspirate eject speed when pipetting normal reagents Reagent speed viscous Defines the aspirate eject speed when pipetting reagents which have been defined as viscous Standards spee...

Page 94: ...olume from their default settings can be useful when preparing master mix for manual PCR setup or when preparing master mix for PCR setup at a later time To change the master mix extra volume settings...

Page 95: ...e 1 Select Set standard extra volume from Options Run settings 2 The Standards Extra Volume dialog box will open 3 Enter a new extra volume Minimum extra volume required Defines the additional volume...

Page 96: ...ram microliter l dollars microliter Custom user defined 5 6 6 Allow use of multiple reagent volumes When activated a tick is visible If activated the user sees the advanced reagents table in the react...

Page 97: ...s three subdirectories Logs Reports and SupportPackages See Section 4 7 for more details about these subfolders To create a new default file location 1 Select Options Run settings Set default file loc...

Page 98: ...ple colors Liquid Colours Enables configuration of liquid reagent colors Selection Colours Enables configuration of colors that represent a liquid function and that represent members of a sample bank...

Page 99: ...QIAgility Software QIAgility User Manual 11 2011 5 37 Liquid Colours Selection Colours Enables selection of a color Adds selected color...

Page 100: ...options To adjust liquid level tracking options 1 Select Options Run settings Use liquid level when pipetting 2 Select 1 of the 3 options Do not use liquid level Detect liquid level using sensor or Us...

Page 101: ...s essential that the plates that are physically located on the instrument worktable are correctly assigned in the software Note The Use liquid level estimate function relies on the assumption that sou...

Page 102: ...the 3 options Interrupt run on all errors Interrupt run on serious error or Ignore all errors Interrupt run on all errors This is the default error level All liquid level tracking errors are reported...

Page 103: ...tip will be used for each aspirate eject cycle This option gives the highest accuracy and precision but requires a large number of tips Run duration will be increased Re use tips where possible Tips w...

Page 104: ...ured Note These options are disabled if a Q Protocol is loaded and locked 3 Select Use each tip for one operation only or select Re use tips where possible from the drop down menu 4 If tip reuse has b...

Page 105: ...og box will open Use air on multi eject Select to blow out air on the final ejection This limits accumulation of liquid on the tip interior This is a default setting when tip reuse is selected but is...

Page 106: ...itial ejection volume 7 Define the Additional Extra Volume Note Setting this value to zero may reduce precision It is recommended to set this volume to half the volume of the aliquot 5 7 Define workta...

Page 107: ...Click to toggle between an enlarged view and a normal view of the current plate type This option has the same function as the Zoom plate button Delete Calibration data Deletes all calibration setting...

Page 108: ...es which match the selection will be listed in the Plate panel Plate Lists plates that match the plate type selection in the Select Plate Type drop down menu Select a plate from this list to view an i...

Page 109: ...ing order and horizontal label order the sequence in which wells are listed in the grid changes according to the well display order selection This function is useful for example when it is convenient...

Page 110: ...ration dialog box will appear 4 Select a plate type from the Select Plate Type drop down menu The Plate list will be automatically updated 5 Select a plate from the Plate list Note If you cannot find...

Page 111: ...ple bank this enables a reaction to be a component of a different reaction A reaction that is a component of another reaction is called an Intermediate reaction Standard A standard is a dilution serie...

Page 112: ...Note Use of an accessory affects the plate height Plate height calibration must therefore be performed with the plate and accessory together To add or remove an accessory 1 Right click on the plate w...

Page 113: ...The instrument performs pipetting operations for the plate segment using the liquid level estimate function See Section 3 3 Use default liquid level mode Tip Sensor The instrument performs pipetting...

Page 114: ...Tip function to select all the tips within that plate Alternatively to select a group of tips left click on the plate and then drag Selected tips will be highlighted red Note To select individual tips...

Page 115: ...or Unavailable repeat steps 1 3 for each group of tips 5 8 Managing samples 5 8 1 Managing sample banks A sample bank is a collection of wells on a given plate or plate segment that is grouped and tre...

Page 116: ...Add first n wells on plate to sample bank Opens the Update Sample Bank dialog box and enables consecutive wells on a plate to be added to a new or existing sample bank Add all wells on plate to sample...

Page 117: ...Set preload volume Enables the user to enter the volume of liquids which have been preloaded into reaction tubes This option is only available when a Reaction plate is selected Click on the OK button...

Page 118: ...st can consist of more than one description e g A1 B2 C2 C6 Bank wells Click to view the existing wells in the sample bank Wells are highlighted red Specify concentration range Select to define a conc...

Page 119: ...he Update Sample Bank dialog box will open 3 Select New Bank and enter a name for the new sample bank 4 If desired left click and drag on the plate schematic in the lower portion of the dialog box to...

Page 120: ...pane Before setting up a reaction list all plate functions must be configured This can be done using the right hand pane The right hand pane refers to the area on the right hand side of the software w...

Page 121: ...e worktable The Tip right hand pane will appear Tip right hand pane 5 9 2 Standards Standard dilutions can be prepared using the QIAgility or a premade dilution series can be used The default location...

Page 122: ...a premade standard dilution series The Set Custom Conc button will become available Standard name Displays the default name of the standard dilution series If desired click in the box and enter a dif...

Page 123: ...the standard is used in at least one reaction configured in the run Set Custom Conc Opens the Custom Concentration dialog box which enables the concentration of dilutions to be defined Click on a cell...

Page 124: ...ncentration values are assumed to be entered in a single unit common to all values in the run The unit itself can be defined as needed for the application See section 5 6 5 Entering concentrations usi...

Page 125: ...a dilution series with varying ratios is required the dilution ratios can be modified To do this click on a cell in the Standard Data table and enter a different dilution ratio Click on the OK tab to...

Page 126: ...me plates have several segments and therefore have more than one option for the location of reagents Reagents can be added directly into reaction tubes or can be used to make up a master mix which can...

Page 127: ...fault volume for all reagents Click on the OK button to continue Name Defines the name of the reagent Names of reagents must be unique within the run Def Vol Defines the volume of reagent to be added...

Page 128: ...is 0 l the reagent can still be used in the master mix component selection but it will not be shown in the reaction configuration 4 When a reagent name is entered the associated well will appear oran...

Page 129: ...of maximal 16 ingredients Note Commercially available master mixes are not premade master mixes because they require addition of primers A commercial master mix and the individual primers must be defi...

Page 130: ...are if unused Sets the minimum volume of master mix to create if the master mix is not used in any reactions in the run This allows the user to create a master mix without assigning it to reactions It...

Page 131: ...r with the liquid in the mixture No of times to mix at end Defines the number of times to mix after addition of the last reagent Configure Mix Opens the Master Mix Component Selection dialog box which...

Page 132: ...ume for this mix How much reagent to manually insert per reaction Defines the volume of reagent that will be manually inserted into each reaction Default reaction volume Defines the default reaction v...

Page 133: ...located in Location Defines the location of the reagent on the plate Name Defines the name of the reagent Use Defines whether the reagent will be used in the master mix Vol per Reaction Defines the vo...

Page 134: ...d in the Reagent right hand pane To change these volumes left click on the corresponding cell in the Vol per Reaction and enter a new volume Click on the OK tab The diluent volume is automatically adj...

Page 135: ...gent Insertion Order dialog box will appear Dn Moves the selected reagent down the list Up Moves the selected reagent up the list Close Closes the Master Mix Reagent Insertion Order dialog box 3 Left...

Page 136: ...2 Select Use Master Mix and then select Use pre mixed Master Mix 3 Enter a name for the master mix in the Master Mix name field 4 Enter the Volume per sample and the Number of times to mix before use...

Page 137: ...eady be in use by previously defined reagents If the name of the reagent used in the saved master mix setup is identical to reagents already set up these will automatically be allocated to those posit...

Page 138: ...must be organized into groups called Sample Banks Each sample can be assigned a name an ID e g a barcode and a starting concentration This information can be imported from an external file e g an Exc...

Page 139: ...mple right hand pane Delete Bank Deletes the selected sample bank Deleting a sample bank will not affect sample names sample IDs or concentrations Rename Bank Opens a dialog box in which the sample ba...

Page 140: ...concentrations are reset to 0 select sample bank A sample bank can be selected from the drop down menu Well Display Order This determines the sequence in which wells are displayed in the sample data g...

Page 141: ...ane displays a list of current Sample Banks Click on the New Bank or Edit Bank buttons to open the Update Sample Bank dialog box Sample banks can be managed via this dialog box See Section 5 8 1 for m...

Page 142: ...ed on their position in the sample plate To change the sample name to input a sample ID or to input a starting concentration left click on a cell in the Sample Name Sample ID or Conc column Directly e...

Page 143: ...action Configuration dialog box Enables a reaction to be edited Alternatively double click on a reaction to open the Reaction Configuration dialog box Copy Copies selected reaction Left click on a rea...

Page 144: ...tinue Displays a summary of the selected reaction Up Dn Modifies the reaction order Left click on the reaction to be changed in the Reaction List Hold down the Shift key to select multiple reactions C...

Page 145: ...tion Info dialog box Clear Clears any user assigned reaction IDs and concentration values Well Display Order This determines the sequence in which wells are displayed in the sample data grid i e Label...

Page 146: ...cells Right click on the selection and select Assign ID s Set Concentrations Enables groups of reactions to be assigned the same value in the Conc column Left click and drag to select a group of cells...

Page 147: ...so no value is displayed If desired the user can assign a concentration value to the reaction well User assigned concentrations are shown in blue This is useful for dilution of samples or stock wells...

Page 148: ...s of liquid handling operations in which the source wells for a given operation correspond to the target wells constructed by a predecessor operation Reaction Configuration dialog box To open the Reac...

Page 149: ...r to the reaction well Mixes and reagents in mix wells Defines the master mixes that will be added to the reaction All wells that have Mix function and that have been assigned a per reaction volume wi...

Page 150: ...automatically set when the operation includes a master mix configured with default sample volume A sample volume of zero is permitted Total volume Defines the per reaction total volume The Total volu...

Page 151: ...s more than 1 component in the reaction Use normalization Instructs the instrument to normalize sample concentrations Auto Adjust small Volumes Instructs the instrument to round up sample volumes that...

Page 152: ...enter the number of times to mix 6 Select the components of the reaction Available master mixes are listed in the Mixes and reagents in mix wells panel and available unmixed reagents are listed in th...

Page 153: ...al options in the reaction setup A number of Special options are available from the Reaction right hand pane To view these options left click on the Special button Note It is recommended that reaction...

Page 154: ...tervention is required to continue the reaction setup Click on the OK button to continue Add fan on Turns the HEPA filter on Add fan off Turns the HEPA filter off Add sample bank pooling step Opens th...

Page 155: ...tion list It can be useful to introduce a pause into the reaction list if an incubation time is required between pipetting operations Note Pauses are processed during sample processing only after mast...

Page 156: ...will appear 3 Click on the Special button 4 Select Add pause until confirmation 5 The Configure Pause dialog box will appear 6 Enter the text to be confirmed by the user 7 Click on the OK button The...

Page 157: ...ing the Add sample bank pooling step option with the default tip usage setting the instrument picks up a tip takes an aliquot from the first plate takes an aliquot from the second plate and then takes...

Page 158: ...contamination may occur Re use tips Instructs the instrument to use a single tip to aspirate and eject each pooling liquid separately This allows pooling of component volumes each up to the largest t...

Page 159: ...e added to the target reaction wells in a pooling step Volume Defines the volume of each sample in the sample bank to be added during a pooling step Add Adds the defined pooling step to the Samples li...

Page 160: ...he Add button 13 When Multi pickup is selected it is recommended to select Air gap from the drop down menu in the Samples panel 14 Define the Volume to be added and then click on the Add button 15 Rep...

Page 161: ...ct Settings Opens the MULTI EJECT dialog box which enables configuration of multi eject options See Section 5 6 12 for more details about how to configure these options Re use tips Instructs the instr...

Page 162: ...ume is dependent on the settings 6 Select the Tip Ejection Mode Multi pickup Re use tips or Use new tips 7 If Multi eject was selected the Multi Eject Settings option will be available See Section 5 6...

Page 163: ...on step This function performs dilutions of a bank of samples with a diluent reagent Each dilution in the series is specified by a pair of volumes that describe the amount of sample and amount of dilu...

Page 164: ...ons Diluent Multi eject Settings Opens the MULTI EJECT dialog box which enables configuration of diluent multi eject options See Section 5 6 12 for more details about how to configure multi eject sett...

Page 165: ...eted Sample volume Defines the volume of the sample bank that will be used in a dilution Diluent volume Defines the volume of diluent that will be used in a dilution Add Adds the dilution step to the...

Page 166: ...and the Diluent volume and then click on the Add button 12 If required repeat steps 8 12 to configure more dilutions Note To remove a copy left click on a copy in the Reaction plates list and then cli...

Page 167: ...le bank to be used in the serial dilution Opens the Plate well Selector screen which enables the target reaction plate to be selected First well Defines the first well of the reaction plate into which...

Page 168: ...erial dilution from the drop down menus 8 Click on the button to select the target reaction plate The Plate well Selector window will appear 9 Left click on a reaction plate to select it The Serial Di...

Page 169: ...normalized samples are prepared in separate reaction wells 1 Left click on a Reaction plate 2 The Reaction right hand pane will appear 3 Click on the Special button 4 Select Add sample bank normaliza...

Page 170: ...lize to Mix count Defines the number of times to mix Use secondary target Instructs the software to prepare a copy of the normalized samples in a second target plate First well Defines the first well...

Page 171: ...plate The Plate well Selector window will appear 8 Left click on a reaction plate to select it The Sample Normalization dialog box will reappear Note If there is only one Reaction plate this plate wil...

Page 172: ...to be moved to waste Opens the Plate well Selector screen which enables selection of the target reaction plate Vessel Defines the vessel in the selected reaction plate to which waste will be moved Us...

Page 173: ...use tips 11 Click on the OK button to return to the main software window The remove waste command will be added to the Reaction List Note With this function more than one sample will be added to one...

Page 174: ...5 Select either New Bank or Existing Bank If New Bank is selected enter a sample bank name If Existing Bank is selected left click on a sample bank to select it 6 Click on the OK button to return to...

Page 175: ...ator Comma is the default when selecting a CSV File Other separators e g tab can be used If needed the ASCII code for any character can be specified making the import function universal Tab The Tab ch...

Page 176: ...maining options in the Import Options panel as desired and select the column from which this information will be imported Note The software will automatically limit the number of rows imported to prev...

Page 177: ...the target plate or segment of the run During the import the wells to which data are imported are added to the selected sample bank Existing members of the sample bank prior to the import are retained...

Page 178: ...ilter function allows the user to filter the imported data limiting the selection of rows prior to import During import only filtered rows presented in the Import Preview pane are included in the impo...

Page 179: ...panel displays the samples to be imported Click the Import button to import the data The imported data will be displayed in the right hand pane Note No data is imported from a row in which all fields...

Page 180: ...the Import button Repeat for each sample bank column in the file Exporting sample and reaction data Sample and reaction data which is listed in the Sample and Reaction right hand pane can be exported...

Page 181: ...ation and a user selectable sample identifier which can be either sample name or sample ID Custom comma separated A csv text file with comma separators between columns The data can be user defined Cus...

Page 182: ...according to special conventions which allow the identification of Detector Reporter Quencher entities required by the ABI SDS software See Appendix E ABI 7000 7500 SDS allelic discrimination A Setup...

Page 183: ...ftware Concentration Option Determines the concentration value to be associated with the reaction for import into the Rotor Gene software Page Option Determines how the reaction data is to be grouped...

Page 184: ...page created for and named after each master mix Reaction ID Reactions are segregated according to the reaction ID user assigned label associated with the well with 1 page created for and named after...

Page 185: ...ment Relative to Segment The well numbers start with 1 at the beginning of the selected segment and proceed in the selected display order label or pipet within the segment Relative to Plate The well n...

Page 186: ...abel e g G3 3 Sample type none unknown standard Sample type none unknown standard 4 Concentration if known Source concentration if known 5 Sample name Final contents including full names and volumes 7...

Page 187: ...xport modes where the data can be freely defined the user must specify the data content by writing an export string During export these designators are substituted with actual corresponding data For e...

Page 188: ...d The PCR Setup Wizard enables typical PCR reactions with samples standards and master mixes to be set up The wizard allows the user to set up reagents and then use these to prepare master mixes The w...

Page 189: ...e of the screens will be used To set up a PCR reaction using the PCR Setup Wizard 1 Select Wizard PCR Setup Wizard or select PCR Setup Wizard from the splash screen that appears when the software is s...

Page 190: ...n See Section 5 7 1 for more details about the available plate functions 4 Left click on a plate to select it Click on the Set Plate button 5 The Plate Configuration dialog box will open Change the pl...

Page 191: ...in the Reagent column and updates all cells in the Viscous column to No 11 To change the reagent name left click on a cell in the Reagent column and then enter a new name To change a reagent from vis...

Page 192: ...e OK button to continue Repeat this step until all reagent segments you want to use are defined 13 Click Next to continue 14 Left click on a well in the Master Mix Block to select the location of the...

Page 193: ...gure the components of the master mix as outlined in Section 5 9 4 16 Click on the OK button to continue 17 The Standards Editor screen will appear Steps 17 to 21 will only appear if you selected I wa...

Page 194: ...ill appear Number of dilution concentrations Defines the total number of wells in the dilution series including the initial undiluted stock well of the standard Concentration of undiluted standard Def...

Page 195: ...ank name can be changed Click on the OK button to continue New Bank Displays the Update Sample Bank dialog box which enables a new sample bank to be created See Section 5 8 1 Edit Bank Displays the Up...

Page 196: ...mix Defines the master mix that will be used in a reaction Standards samples Defines the standard or sample that will be used in a reaction iteration s Defines the number of reaction replicates that...

Page 197: ...ard or sample from the second drop down menu and define the number of replicates in the field below 29 Click on the button to define the Target plate 30 Click on the Add button The reaction will now b...

Page 198: ...screens that will guide the user through the setup Instructions are displayed on each screen Note The Optimisation Wizard only provides steps to set up an optimized matrix it does not provide steps fo...

Page 199: ...oncentrations would you like to use Defines the number of different concentrations of reagent to be optimized Set Locations Opens the Reagent selector window which enables selection of the location of...

Page 200: ...ocations do not matter click and drag the Auto white box to the desired reagent segment Reagents will automatically be assigned to a location Selected wells will be highlighted red Note Some reagent l...

Page 201: ...lected in step 3 repeat steps 7 13 and then proceed to step 15 15 The Optimisation Wizard Replicates dialog box will appear How many replicates would you like to produce Defines the number of replicat...

Page 202: ...en this option is not selected the wizard appends the optimization reactions to the existing reaction list 16 Define the number of replicates for each reaction 17 Define the master mix to be used in t...

Page 203: ...pear when starting the software and can also be accessed by selecting File New 2 Select either Empty Project last used layout to use the layout from the most recent run or Empty Project default layout...

Page 204: ...n list See Section 5 9 6 for details about how to do this In addition see Section 5 10 4 for examples of how to set up typical reactions 8 To save the run file select File Save As Enter a name for the...

Page 205: ...Enter the number of replicates in the Target wells panel 4 Click on the OK button Master mix with sample bank 1 Select the sample bank from the Samples panel 2 Select the master mix 3 Enter the numbe...

Page 206: ...wells panel 5 Click on the OK button No template control with master mix 1 Select Diluent from the Samples panel 2 Select the master mix 3 Enter the number of replicates in the Target wells panel 4 Cl...

Page 207: ...ighted 5 10 6 Required volumes for reaction setup The software automatically calculates the volume of standard diluent reagents and master mix required for a reaction setup To view the volume of liqui...

Page 208: ...vice register describes the current status of maintenance alert notification records that are associated with the instrument The software maintains a schedule of required service activities in the ser...

Page 209: ...ervice at a future date Component Defines the selected component to be serviced Pipettor or Hepa Filter Due Date Defines the date that a service is required Acknowledg ment Date Displays the current d...

Page 210: ...cknowledgment 7 The service that has been acknowledged will now be colored black in the Service Register dialog box In addition the component will be colored in green as a reminder of a future service...

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Page 213: ...the current movement which may take up to 10 seconds WARNING CAUTION Risk of personal injury and material damage W4 Do not attempt to move the QIAgility during operation WARNING Moving parts W12 To av...

Page 214: ...ures 6 2 QIAgility User Manual 11 2011 computer or if the instrument is switched off the software will run in Virtual Mode See Section 5 2 for more details about Virtual Mode operation 3 The welcome d...

Page 215: ...e displayed left click on a run file to select it and then click on the Open button Alternatively double click on the run file to open it directly 4 The main software window will open See Section 5 3...

Page 216: ...tes using the arrows Start Initiates UV lamp operation Close software when UV finishes Shuts down the QIAgility Software when UV lamp operation is complete Enable Lamp Switches the UV lamp on Disable...

Page 217: ...will appear green 3 To switch the HEPA filter off click on the icon in the toolbar 6 4 Executing a run 6 4 1 Q Protocols Q Protocols are run files which have been developed to directly facilitate spec...

Page 218: ...If the user attempts to overwrite an existing Q Protocol the following message will appear Using a Q Protocol 1 Start the QIAgility software as outlined in Section 5 1 Note If the QIAgility software...

Page 219: ...nual 11 2011 6 7 4 Select the Protocols tab All Q Protocols that are saved in the appropriate folder i e QProtocols will be displayed 5 Select the desired Q Protocol 6 Click on the Open button 7 A des...

Page 220: ...Section 5 7 2 modify sample banks see Section 5 8 1 assign plate IDs barcodes see Sections 5 9 5 5 9 6 and 5 9 7 and assign well IDs barcodes to sample reaction wells see Sections 5 9 5 5 9 6 and 5 9...

Page 221: ...u 1 Select File from the menu bar Recently used run files are listed in the File menu 2 Select one of these files 6 4 3 Creating a new run file There are 2 wizards PCR Setup Wizard and Optimisation Wi...

Page 222: ...uids that are required on the worktable for completion of the loaded run file To view the pre run report select Wizards Generate report See Section 6 7 1 for more details about the pre run report Note...

Page 223: ...the cursor over the source well A hover box will appear which displays the required volume of liquid that the software has calculated This volume includes the extra volume which allows for liquid rec...

Page 224: ...ort Check the worktable setup 6 Click on the OK button to start the run 7 The location of the pipetting head will be highlighted on the software worktable in real time and a summary of the progress of...

Page 225: ...aborted run cannot be restarted from the point at which it was aborted 6 6 Exception handling If an exception i e an error or warning occurs during a run an alarm will sound for 60 seconds and then at...

Page 226: ...ps 1 Add the correct tips to one of the available tip locations on the worktable 2 Select the Tip plate where the tips have been replaced by checking the box next to the correct plate 3 Click on the O...

Page 227: ...a new e mail message which enables the pre run report to be e mailed Saves the pre run report as a Word document Assign a file name select a location and then click on the Save button Closes the pre r...

Page 228: ...re Run Report button The pre run report will be displayed 6 7 2 Post run report Upon completion of a run a Post run report will appear This report contains the same information as the Pre run report b...

Page 229: ...rectory called Reports in the current run file save directory The default directory is C Program Files QIAgility Data 6 7 3 Log files and support packages Log files A new log file is generated when th...

Page 230: ...blems that occur outside the context of a run it is important to manually create the support package immediately after a suspected error has occurred Support packages are important when reporting a pr...

Page 231: ...eption User paused job Displays all support packages in which the user paused the run file User aborted job Displays all support packages in which the user aborted the run file 3 The support packages...

Page 232: ...top right corner of the screen or select File Exit to shut down the software 2 If a run is still active the software will display a warning message 3 Select Return to Run if you want to cancel the sh...

Page 233: ...Position recommended was selected the robot will discard the tip and the pipetting head will now move to a safe location The following message will appear 13 Click on the OK button 14 Set the power s...

Page 234: ...mmediately This is a faster way of shutting down The instrument axes remain engaged and the pipetting head remains in an upward position If the head holds a tip it will not be discarded As the instrum...

Page 235: ...curate there is no need to recalibrate the volume Note If pipetted volumes are found to be inaccurate please contact QIAGEN Technical Services 7 1 1 Setup tip ejector Before starting ensure that the w...

Page 236: ...red 7 Click on the Yes button The automated calibration procedure will start 8 Once the X and Y axis positions have been located the following message will appear 9 Check the pipetting hub position If...

Page 237: ...utton The following dialog box will appear 11 Manually adjust the X and Y axis positions using the arrows and then click on the Locate Z Axis button 12 The following message box will appear 13 Click o...

Page 238: ...jector calibration 7 1 2 Calibrate plate positions Before using a plate on the worktable a position calibration must be performed Plate position calibrations can be saved and reused Important Tip Rack...

Page 239: ...d on the X axis During calibration the objective is to set this to match the center position of the calibration well Y position Indicates the position of the pipetting head on the Y axis During calibr...

Page 240: ...ove the pipetting head so that it is just above the plate surface Ensure that the tip is not in contact with the plate Note If there is no tip attached the pipetting head can only move to a limited Z...

Page 241: ...l tips are available that the Tip Rack Holder is calibrated and that the correct tips are set as available Ensure that empty tubes are placed in the first 4 wells of each plate 1 Select Options Robot...

Page 242: ...tes Abort Aborts the current calibration activity Manual Setup highlighted items Initiates the manual plate height calibration operation Manual plate height calibration is performed only for the selec...

Page 243: ...ating the calibration ensure that all tubes are sitting correctly and that the tips are not bent Note If there is significant variation across the 4 samples the following message box will appear Click...

Page 244: ...the plate and bend the tip Raise tip to 0 Lifts the pipetting head to the current 0 position Lower tip to current setpoint Places the pipetting head at the height that corresponds to the displayed Lev...

Page 245: ...the true distance of the base of the tip from the home position Use the Home Z axis button to reset the 0 position and repeat steps 5 7 starting with a lower Level at bottom of well value to avoid dr...

Page 246: ...g the procedure Ensure that a plate with Reaction or Mix function with well sizes large enough to accommodate 50 l and 200 l tips is available this plate must be position and height calibrated Place a...

Page 247: ...hich tip offset calibration will be performed Select tip types to profile Lists the tip types to be calibrated 200 l conductive tips are selected by default Test how many tips per tip type Defines the...

Page 248: ...the average offset 7 Click on the Start button to start tip offset calibration 7 1 6 Calibrate volumes There is a direct relationship between the rotation of the motor shaft and the vertical movement...

Page 249: ...an Empty Project last used layout or Empty Project default layout as outlined in steps 1 2 of Section 5 10 3 2 Select Options Robot Setup Calibrate Volumes 3 The Volume Calibration Wizard dialog box w...

Page 250: ...ng the P axis value increases the volume aspirated or dispensed Conversely decreasing the P axis value decreases the volume aspirated or dispensed 1 Select Option 1 from the Volume Calibration Wizard...

Page 251: ...played settings as new calibration Updates the default volume calibration with the displayed calibration settings 8 Current Settings are displayed by default Saved calibration settings up to 9 can be...

Page 252: ...bration Wizard History of Calibration Data dialog box 12 Click on the Finish button to return to the main software window Option 2 Use Option 2 to view current calibration settings and to replace the...

Page 253: ...tings Current calibrations are automatically saved in the volume calibration history 1 Select Option 3 from the Volume Calibration dialog box 2 The following message will appear 3 Click on the Yes but...

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Page 255: ...this user manual Servicing The QIAgility is supplied with a warranty that lasts for 1 year beginning on the date of shipment The warranty includes all repairs due to mechanical breakdown Application d...

Page 256: ...e instrument lid Alcohol will damage the lid To clean the lid use distilled water only CAUTION Damage to the QIAgility side panels C6 Never clean the instrument side panels with alcohol or alcohol bas...

Page 257: ...nts in TriGene Medichem International for more information see www medi mark co uk or one of the tested cleaning agents listed in Section 8 1 1 above for 15 30 min 3 Thoroughly rinse the components in...

Page 258: ...ip ejector chute Removing the tip ejector chute 1 Open the lid 2 Grasp the chain connected to the tip ejector chute 3 Using the chain pull out the sliding security bolt 4 Remove the tip ejector tip ej...

Page 259: ...ress the sliding security bolt into the side slot opening 8 2 3 Replacing the HEPA filter 1 Lift the HEPA filter lid section 2 Remove the lid 3 Unscrew the 4 thumb screws 4 Remove the H Frame 5 Remove...

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Page 261: ...he computer and the instrument Check the connection of the USB or RS 232 cable See Section 4 5 b Incorrect COM port settings Check the COM port setting If the instrument is connected to the incorrect...

Page 262: ...en configured properly Tip reuse causes greater liquid losses on the insides of tips due to capillary and surface tension effects Disable tip reuse Master mix may be viscous and build up on the outsid...

Page 263: ...Screen savers and power save modes not disabled on the computer Disable screen savers and power save modes on the computer Z axis not rising after power has been switched on a Power cords not securely...

Page 264: ...Incorrect tip configuration The instrument may have been configured to pick up tips that were not present in the Tip Rack Holder Ensure that tip configuration is correct b Incorrect plate height calib...

Page 265: ...sage is received when the software is started Correct plate definitions not installed Uninstall and then reinstall the software See Section 4 6 Software does not start and the A required library file...

Page 266: ...age describing the error or warning The run can commence with active warning messages but this may lead to erroneous results Note Warnings and errors are logged in the post run report and in any suppo...

Page 267: ...s lower than the extra volume recommended to avoid shortages Standards volume too high An instrument prepared standard well has a volume that is too large for a 200 l tip to mix effectively Speed not...

Page 268: ...equired volume are not available Volume calibration must be done before run Volume calibration must be done before a run can be started in these situations New installation of instrument After pipetto...

Page 269: ...OM 129 An invalid axis was specified 130 The instrument did not respond with the first reply 131 The instrument did not respond with a second reply 132 The COM port has problems 133 The micro firmware...

Page 270: ...147 A bad packet length was received 148 More data was received while processing 9 4 Self diagnostic tools These tests are designed to collect information to help with troubleshooting Note These test...

Page 271: ...e Pipettor Head See Section 9 4 1 for more details Check Commun ications See Section 9 4 2 for more details Liquid Sensor Test See Section 9 4 2 for more details Close Closes the dialog box Go Home Ho...

Page 272: ...about how to save a support package Cycle Pipettor Head After calibration or if the instrument has been unused for a long period of time it is recommended to cycle the pipetting head This helps to dis...

Page 273: ...11 9 13 appears during the test there is no problem with the communications links Liquid Sensor Test This test checks that the liquid sensor circuitry is functioning correctly so that it only triggers...

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Page 275: ...t volume of the reaction Master Mix Block The Master Mix Block is intended to hold diluent and master mix on the instrument worktable Menu bar The menu bar contains 5 menus File Control Wizards Option...

Page 276: ...ht side of the software workspace It provides access to options which relate to different plate functions When a run is in progress a progress report is displayed in the right hand pane Run file A run...

Page 277: ...e pipetting head is highlighted on the software worktable Standard A standard is a dilution series with known concentrations Standards are treated like samples Virtual Mode Virtual Mode enables run fi...

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Page 279: ...ctuations are not to exceed 10 of the nominal supply voltages Fuse F5A 250 V fuse Overvoltage category II Air temperature 15 30 C 59 86 F Relative humidity 40 70 Altitude Up to 2000 m 6500 ft Place of...

Page 280: ...mensions Width 54 cm 21 3 in Depth 63 cm 24 8 in without cables 72 cm 28 3 in with cables Height 45 cm 17 7 in lid closed 86 cm 33 9 in lid open Mass 41 kg 90 2 lb standard configuration Capacity Up t...

Page 281: ...f life The separate collection and recycling of waste electronic equipment at the time of disposal helps to conserve natural resources and ensures that the product is recycled in a manner that protect...

Page 282: ...ply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is oper...

Page 283: ...responsibility that the product QIAgility meets all applicable requirements of the following European Directives Low Voltage Directive LVD Electromagnetic Compatibility Directive EMC 2006 95 EC 2004 1...

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Page 285: ...for the duration of the original warranty period and in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices inte...

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Page 287: ...s de s curit suivants sont fournis tout au long du manuel AVERTISSEMENT Le terme AVERTISSEMENT signale des situations risquant d entra ner des accidents corporels dont l utilisateur ou d autres person...

Page 288: ...GEN AVERTISSEMENT ATTENTION Risque de surchauffe W2 Afin de garantir une bonne ventilation laisser un d gagement d au moins 10 cm sur les c t s et l arri re du QIAgility Les fentes et les ouvertures q...

Page 289: ...il et ab mer les l ments en plastique Tenir le QIAgility l abri de la lumi re directe du soleil ATTENTION D t rioration de l appareil C2 viter de renverser de l eau ou des produits chimiques sur le QI...

Page 290: ...sous tension AVERTISSEMENT Danger d irradiation UV W9 Un dispositif lectronique de verrouillage emp che la lampe UV de fonctionner tant que le capot de l appareil est ouvert Ce dispositif ne doit en a...

Page 291: ...ologique Les sp cimens et les r actifs contenant des mati res provenant d tres humains doivent tre consid r s comme potentiellement infectieux Utilisez des proc dures de laboratoire s res comme d crit...

Page 292: ...convenablement form s et ne sont pas expos s des niveaux dangereux d agents infectieux comme cela est d fini dans les fiches techniques sant s curit MSDS ou dans les documents de l OSHA de l ACGIH ou...

Page 293: ...ise au rebut des d chets doivent s effectuer conform ment toutes les r glementations et lois nationales r gionales et locales relatives la sant et la s curit OSHA Occupational Safety and Health Admini...

Page 294: ...de mani re satisfaisante et en toute s curit suivre les conseils suivants Utiliser uniquement les tips et les supports de tips recommand s Dans la mesure du possible maintenir le capot de l appareil f...

Page 295: ...lity Risque d accident corporel et de d t rioration du mat riel Effectuer uniquement la maintenance sp cifiquement d crite dans ce manuel ATTENTION D t rioration de l appareil C4 Ne pas utiliser de so...

Page 296: ...le de danger biologique Table de travail Symbole de risque d crasement Plaque signal tique l arri re de l appareil Marque CE Plaque signal tique l arri re de l appareil Label CSA pour le Canada et les...

Page 297: ...appareil Label C Tick pour l Australie identifiant du fournisseur N17965 Plaque signal tique l arri re de l appareil Label RoHS pour la Chine restriction de l utilisation de certaines substances dang...

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Page 299: ...genden Kategorien von Sicherheitshinweisen verwendet WARNUNG Der Begriff WARNUNG WARNING weist Sie auf Situationen hin in denen eine Verletzungsgefahr f r Sie selbst oder andere Personen besteht N her...

Page 300: ...R ckseite und Seitenteile ein Mindestabstand von 10 cm eingehalten wird Das gew hrleistet eine ausreichende Bel ftung des Ger tes Die L ftungsschlitze und ffnungen die f r die Be und Entl ftung des QI...

Page 301: ...hemikalien auf der Oberfl che des QIAgility zu versch tten Durch versch ttete Chemikalien oder versch ttetes Wasser verursachte Ger tesch den sind nicht durch die Garantie abgedeckt WARNUNG Verletzung...

Page 302: ...lektronisch gesteuerte Verriegelung stellt sicher dass die Ger tehaube geschlossen sein muss bevor die UV Lampe eingeschaltet werden kann Diese Verriegelung sollte auf keinen Fall deaktiviert werden U...

Page 303: ...g A angegeben gelagert wurde das Ger t unsachgem transportiert worden ist 1 3 Biologische Sicherheit Bei Substanzen und Reagenzien die humanes Untersuchungsmaterial enthalten sollte immer von einer m...

Page 304: ...Arbeits platzes sicher ist und die Bediener des Ger ts ausreichend geschult sind Au erdem d rfen die Grenzwerte in Bezug auf infekti se Erreger die in den entsprechenden Sicherheitsdatenbl ttern MSDS...

Page 305: ...Beim Betrieb des Abzugs und bei der Entsorgung von Abfallstoffen m ssen alle Bestimmungen und Gesetze zu Gesundheitsschutz und Sicherheit am Arbeitsplatz auf bernationaler nationaler und regionaler Eb...

Page 306: ...dringend einen Neustart des Systems durchzuf hren falls der Y Arm versehentlich von Hand bewegt wurde Um einen zufriedenstellenden und sicheren Betrieb des QIAgility zu gew hrleisten befolgen Sie bitt...

Page 307: ...eiten gem den Anweisungen in Abschnitt durch QIAGEN stellt alle Reparaturen in Rechnung die nachweislich auf eine inkorrekte Wartung zur ckzuf hren sind WARNUNG ACHTUNG Verletzungsgefahr und Besch dig...

Page 308: ...eagenzien die S uren Laugen oder Abrasivstoffe enthalten um die QIAgility Haube zu reinigen Alkohol sch digt die Ger tehaube Verwenden Sie zum Reinigen der Ger tehaube ausschlie lich destilliertes Was...

Page 309: ...der R ckseite des Instruments CE Kennzeichnung Typenschild auf der R ckseite des Instruments Symbol der CSA Zertifizierung in Kanada und den USA Typenschild auf der R ckseite des Instruments Herstell...

Page 310: ...truments C Tick Mark Zeichen f r Australien Herstellerkennung N17965 Typenschild R ckseite des Instruments Markierung gem RoHS Richtlinie f r China Beschr nkung der Verwendung bestimmter gef hrlicher...

Page 311: ...ing of characters which excludes Is a separator D Is a placeholder for detector R Is a placeholder for reporter Q Is a placeholder for quencher Indicates an optional component Indicates 0 or more item...

Page 312: ...tector for CMV using FAM reporter and TAMRA quencher and detector for EBV using JOE reporter and TAMRA quencher Mix D represents the marker name in the SDS software The allelic discrimination function...

Page 313: ...ng Three Stage Instructs the instrument to collect tips in 3 separate downward movements 1 The pipetting head is brought into the tip collar 2 The pipetting head is gently pressed against the tip coll...

Page 314: ...dress a variety of situations where the tip would otherwise be placed too high in the well for pipetting operations Note Using this function may increase the amount of liquid carried on the exterior o...

Page 315: ...s reaction wells sequentially from the first available well in the target plate not used by previous reaction setup operations in the run When activated the software attempts to use the well index in...

Page 316: ...well while aspirating thereby approximately tracking the liquid surface during the operation By default this function is not activated Retract Z axis whilst dispensing When activated a tick is visibl...

Page 317: ...ly causes insufficient volume to be aspirated and hence a deficiency in volume transferred to the target well This function presents options to reduce the impact of false triggering caused by static e...

Page 318: ...ed tube well height more closely to the true internal base of the vessel Details are described below While this may reduce the wasted dead volume it also increases the risk that liquid may not be foun...

Page 319: ...list Clear All Deselects all of the plates in the Plates to Calibrate list Require confirmation from user Instructs the software to present a message to the user after automatic plate height calibrati...

Page 320: ...rate list Alternatively click on the Select All button to select all of the plates Note Plates which have multiple segments e g Reagent Blocks are listed more than once but with different tube types 4...

Page 321: ...for each vessel to be measured Where the segment is not eligible for optimal height calibration a single tip is used to probe the base of the vessel and the process proceeds as described in Section 7...

Page 322: ...termined height calibration value is automatically saved 10 If more than one plate was selected in the Plates to Calibrate list the instrument will calibrate each plate in turn Repeat steps 8 and 9 fo...

Page 323: ...abel WellID WellNo 1 WellNo StandardWellName A 1 StandardWellName Color 5775359 Color TubeType BioSprint_250 TubeType Concentration 37 Concentration FinalWellVol 80 FinalWellVol EventOccurred 0 EventO...

Page 324: ...g Create sample banks from target wells As the XML export can be used to export more than one plate the Socket tag might be repeated for every exported plate data It is possible to create XSL transfor...

Page 325: ...Appendix G QIAgility User Manual 11 2011 G 3 EmptyLabelType Possible values EL_Empty 0 EL_WellID 1 EL_WellOrder 2 EL_WellNumber 3 They can be evaluated during the transformation...

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Page 327: ...the QIAgility package the instrument as follows 1 Open the hood and remove all plates and tips from the worktable 2 Shut down the QIAgility Software and switch off the QIAgility as outlined in Sectio...

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Page 329: ...er 3 14 connecting 4 17 Conductive Filtered Tips 3 12 D Decontamination 8 3 Dilution series 5 63 E Errors 6 13 9 6 error codes 9 8 Exporting data 5 111 F Feet 3 3 Fumes 1 5 G Generic XML Format G 1 Gl...

Page 330: ...otocols folder 4 16 QIAgility hardware features A 2 internal features 3 8 mechanical data A 2 R Reagent Block 3 10 Right hand pane mix 5 66 progress report 5 126 reaction 5 80 reagent 5 64 sample 5 76...

Page 331: ...ailability 5 52 tip usage 5 41 toolbar 5 13 uninstalling 4 14 virtual mode 5 2 Storage conditions A 1 Support packages 6 18 Symbols 1 8 T Technical assistance 2 2 Tip chute 3 4 8 4 Tip disposal box 3...

Page 332: ...Index Index 4 QIAgility User Manual 11 2011 This page intentionally left blank...

Page 333: ...echnical 0800 914413 France n Orders 01 60 920 926 n Fax 01 60 920 925 n Technical 01 60 920 930 n Offers 01 60 920 928 Germany n Orders 02103 29 12000 n Fax 02103 29 22000 n Technical 02103 29 12400...

Page 334: ...Technical 0800 914413 France n Orders 01 60 920 926 n Fax 01 60 920 925 n Technical 01 60 920 930 n Offers 01 60 920 928 Germany n Orders 02103 29 12000 n Fax 02103 29 22000 n Technical 02103 29 12400...

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