Qiagen QIAgility, User Manual

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QIAgility Software
QIAgility User Manual 11/2011 5-39
Use liquid level
estimate
Instructs the instrument to use liquid-level
estimate to track the liquid level. See
Section 3.3.
Do not use
liquid level
The instrument performs pipetting
operations at the height calibration position
for the plate/tube.
Note : These options are disabled if a Q Protocol is
loaded and locked.
Warning : “Do not use liquid level” is not recommended
for general use because liquid is more likely to be
retained on the tip exterior. Pipetting accuracy and
precision may therefore be compromised when using this
option. This option is intended for use only in exceptional
circumstances.
Note : For correct function of the liquid-level sensor, it is
essential that the plates that are physically located on the
instrument worktable are correctly assigned in the
software.
Note : The “Use liquid level estimate” function relies on
the assumption that source wells are initially supplied
with the volume of liquid that has been calculated by the
software. Overfilling the source wells can cause excess
liquid to be retained on the tip exterior. Underfilling the
source wells causes insufficient volumes to be aspirated
and then pipetted.
3. A tick will appear next to the option that has been
selected.
Note : The global liquid level tracking options defined here
can be overwritten locally per plate segment. (See also “Set
liquid level mode for segment” in Section 5.7.1.)
5.6.10 Set liquid-level tracking error level
If a tube contains insufficient liquid to complete a reaction
setup, an error message will appear. User intervention will
be required to continue with the run.