Qiagen QIAcuity 911000, User Manual

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QIAcuity User Manual 06/2022 284
Glossary
Glossary terms are listed in alphabetical order.
Term Description
Acquisition The collection of fluorescent data at the end of the run
Channel A channel consists of a light emitting diode (LED) with an excitation filter paired with an
emission filter
The LED and excitation filter excite samples at a given wavelength. Fluorescence emitted by
samples is passed through the emission filter, before being detected by the camera.
Confidence Interval Indicates the range of values that is likely to contain the true parameter value
dPCR parameters Parameters specifying a PCR run (e.g., number of cycles, temperature, acquisitions, etc.)
Environment
The QIAcuity Software Suite consists of several environments (e.g., “Plates”, “Templates”,
“Analysis”, “Report”). In these environments, certain tasks can be performed, such as
setting up a run or analyze data.
Error code A 3- or 4-digit number that indicates an error of the QIAcuity
Exposure duration The length of time the samples are exposed to light during the fluorescens acquisition
Gain A setting to amplify the fluorescence signal
If the gain is set too high, the signal is oversaturated. If the gain is set too low, it is not
possible to differentiate signal from background noise.
GUI Graphical user interface
Initialization An operation performed automatically when the QIAcuity is switched on or by initiating a
self-check of the instrument, if required
Nanoplate dPCR plate with several single partitions
Optical configuration The optical configuration of a QIAcuity instrument is described by the available channels to
detect fluorescence signals. The optical configuration differs between different types of the
QIAcuity instruments.
Partition Compartment in the Nanoplate where the PCR reaction takes place
Plate seal Foil to be applied on top of the plate to prevent evaporation and contamination
Power switch A button located at the front of the QIAcuity in the bottom-right corner
It allows the user to switch the QIAcuity on and off; inner position is ON and outer position
is OFF.
Priming Filling of the partitions with the reaction volume
Rolling Separation of the single partitions filled with the reaction volume
Support Package Information wrapped up in a *.zip file to be sent via an email program to QIAGEN Technical
Services to inform QIAGEN what went wrong at the customer's site and how to help the customer
Touchscreen The user interface that allows the user to operate the QIAcuity
VPF Volume Precision Factor. The VPF specifies the exact cycled volume of a well within a
Nanoplate and therefore further increases precision of concentration calculation in each well.