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QIAcube HT
User Manual

For use with the QIAcube HT Prep Manager Software

Sample to Insight

December 2017

Summary of Contents for QIAcube HT

Page 1: ...QIAcube HT User Manual For use with the QIAcube HT Prep Manager Software Sample to Insight December 2017...

Page 2: ...he QIAcube HT 1 4 1 5 Requirements for QIAcube HT users 1 4 1 6 Requirements for some kits 2 2 2 Safety information 2 2 2 1 Proper use 2 4 2 2 Electrical safety 2 5 2 3 Biological safety 2 3 1 2 5 Sam...

Page 3: ...1 4 3 Installation site 4 2 2 4 4 Power requirements 4 2 3 4 4 Grounding requirements 4 5 4 3 Unpacking and installation 4 3 1 4 5 Unpacking the QIAcube HT 4 6 Releasing transport locks 4 3 2 4 7 Inst...

Page 4: ...bration 5 6 2 5 21 Assembly for standard purification protocols 5 23 5 7 Instrument calibration 5 7 1 5 25 Calibrate volumes 5 7 2 5 27 Calibration positioning buttons 5 7 3 5 28 Calibrate tip racks 5...

Page 5: ...24 Run step 6 25 Open system test 6 25 Plate detection test 6 26 Pausing Stopping a run 6 27 Vacuum performance check 6 29 Run completion 6 30 6 2 Finishing work and shutting down 7 7 2 QIAcube HT Pre...

Page 6: ...riments 7 2 1 7 31 Manage experiments and templates 7 31 Create a template 7 32 Manage favorite templates 7 34 View recent experiments 7 2 2 7 35 Creating an experiment 7 36 Create an empty experiment...

Page 7: ...nstrument 8 4 2 8 12 Maintenance schedule 8 4 3 8 15 Maintenance of the QIAcube HT pipettor 8 4 4 8 15 Replacing the HEPA filters 8 4 5 8 18 Replacing filters on vacuum station 9 9 2 Troubleshooting 9...

Page 8: ...11 3 5 11 10 Traitement des d chets 11 3 6 11 11 Dangers m caniques 11 3 7 11 12 S curit relative la maintenance 11 3 8 11 14 Symboles sur le QIAcube HT 11 15 11 4 Appendix D Sicherheitshinweise 11 4...

Page 9: ...9 QIAcube HT User Manual 12 2017 11 6 1 11 26 License Terms 11 6 2 11 63 Copyright information...

Page 10: ...Introduction QIAcube HT User Manual 12 2017 1 1...

Page 11: ...using the QIAcube HT Prep Manager Software This user manual is also applicable to the QIAxtractor instruments that have been upgraded to QIAcube HT using the QIAcube HT Prep Manager Software 1 2 Abou...

Page 12: ...Support Center at www support qiagen com 1 3 2 Policy statement It is the policy of QIAGEN to improve products as new techniques and components become available QIAGEN reserves the right to change sp...

Page 13: ...ly trained and experienced personnel familiar with use of computers and automation in general Routine use running experiments Laboratory technicians or equivalent Appropriately trained and experienced...

Page 14: ...Safety information QIAcube HT User Manual 12 2017 2 1...

Page 15: ...a box like this one CAUTION The term CAUTION is used to inform you about situations that could result in damage to the instrument or other equipment Details about these circumstances are given in a bo...

Page 16: ...5 The QIAcube HT is not designed for use in an explosive atmosphere WARNING Risk of explosion W6 The QIAcube HT is intended for use with reagents and substances supplied with QIAGEN kits Use of other...

Page 17: ...Intentional interruption is prohibited Lethal voltages inside the instrument When the instrument is connected to line power terminals may be live and opening covers or removing parts is likely to expo...

Page 18: ...nd should use store and dispose of such samples according to the required safety regulations WARNING Samples containing infectious agents W10 Some samples used with this instrument may contain infecti...

Page 19: ...of America ACGIH American Conference of Government Industrial Hygienists United States of America COSHH Control of Substances Hazardous to Health United Kingdom 2 4 1 Toxic fumes If working with vola...

Page 20: ...n always stand clear of the instrument when making adjustments The lid sensor is disabled during calibration Therefore do not click with the mouse or press any of the keys on the keyboard when looking...

Page 21: ...t use spray bottles containing alcohol or disinfectant to clean surfaces of the QIAcube HT CAUTION Damage to the QIAcube HT lid C5 Do not use alcohol solvents reagents containing acids alkalis or abra...

Page 22: ...should the transfer carriage for use with filter plates be autoclaved CAUTION Relocating the QIAcube HT C10 The most important component of the packaging is the wooden support that immobilizes the Y a...

Page 23: ...mark for Canada and the USA Type plate on the back of the instrument Legal manufacturer Type plate on the back of the instrument Waste Electrical and Electronic Equipment WEEE Type plate on the back...

Page 24: ...General description QIAcube HT User Manual 12 2017 3 1...

Page 25: ...lls tissues and food material as well as from bacteria and viruses in animal and human samples Automated protocols and dedicated QIAcube HT purification kits and plasticware increase reliability and c...

Page 26: ...instrument The QIAcube HT 1 Lid 7 Reagent plate R1 and troughs 2 UV lamp 8 Lysis plate 3 Pipettor head robotic arm 9 10 Vacuum chamber with filter plate 4 Tip chute 11 HEPA filter 5 Tip racks 12 Acces...

Page 27: ...ng the instrument lid while the instrument is performing a run will cause the run to pause The instrument lid may remain open during calibration Do not interfere with robotic arm movement during these...

Page 28: ...removal for cleaning 3 1 4 Tip disposal box Discarded tips are collected in a tip disposal box located at the side of the QIAcube HT To ensure correct positioning under the tip chute the tip disposal...

Page 29: ...r system provides positive clean air pressure on the worktable beneath the lid The HEPA filter system consists of two HEPA filter cartridges 3 1 6 UV lamp A UV lamp in the instrument lid provides effi...

Page 30: ...s attach to the pipettor pistons of the pipettor head The robotic arm controls the movement of the pipettor head in the X Y and Z directions The Y axis arm of the robotic arm provides support for the...

Page 31: ...s to ensure that correct orientation is maintained The preceding picture of the QIAcube HT worktable shows the usual positions of the clamps clamp II between B1 and B2 and clamp I between C1 and C2 wi...

Page 32: ...accessory panel on the rear of the QIAcube HT QIAcube HT accessory panel 1 Accessory 1 6 Accessory power outlet 2 Accessory 2 7 Main power input socket 3 Accessory 3 8 Power switch 4 Serial RS 232 soc...

Page 33: ...ion during purification For details about the assembling of the vacuum chamber see Section Assembling the vacuum chamber The waste and elution chambers of the vacuum chamber are connected to the waste...

Page 34: ...ust not be modified e g shortened or lengthened The transfer carriage provides a platform for transporting the filter plate between the waste and elution chambers of the vacuum chamber Transfer carria...

Page 35: ...QIAcube HT User Manual 12 2017 3 12 3 1 11 Vacuum control station and waste bottle Vacuum control station...

Page 36: ...Vacuum pressure is precisely controlled by the QIAcube HT Prep Manager Software A 4 liter waste bottle is housed in the vacuum control station The waste bottle is connected to the vacuum chamber by a...

Page 37: ...ttle volume level is indicated as one of three states in the software worktable screen When the state is green or yellow a run can be started When the state is red the user will be prompted to empty t...

Page 38: ...omatically selected when the pinch valve is switched on This position protects the silicone hoses controlled by the pinch valve from potential damage caused by constant pressure when the instrument is...

Page 39: ...QIAcube HT User Manual 12 2017 3 16 3 1 13 Internal features of the QIAcube HT...

Page 40: ...12 2017 3 17 1 X axis 7 Worktable 2 Y axis 8 Reagent plate SBS and troughs 3 Z axis 9 Reagent plate R1 and troughs 4 Pipettor head 10 Tip racks and tips 5 Vacuum elution chamber 11 Y arm 6 Vacuum wast...

Page 41: ...re during sample purification In addition the worktable supports a special Reagent plate for R1 position 3 2 Computer The QIAcube HT is operated via a computer A laptop with the correct specification...

Page 42: ...er an installation of Windows security patches to prevent automatic computer restart during the operating times of the QIAcube HT Note In order to complete a security update installation perform a man...

Page 43: ...Installation procedures QIAcube HT User Manual 12 2017 4 1...

Page 44: ...ent Trough R1 Position 1x Adapter Reagent Trough SBS Plate The first three adapters are intended to be used in the vacuum chamber to hold a filter plate and to ensure optimal liquid flowthrough and el...

Page 45: ...s final position The requirements for the QIAcube HT location and position are given below The location must have sufficient space to accommodate the QIAcube HT Refer to Appendix A for instrument dime...

Page 46: ...ings that ensure the ventilation of the QIAcube HT must not be covered CAUTION Damage to the instrument C1 Direct sunlight may bleach parts of the instrument and cause damage to plastic parts The QIAc...

Page 47: ...ument needs to be cleaned before it can be packed for transport WARNING CAUTION Risk of personal injury and material damage W3 The QIAcube HT is too heavy to be lifted by one person To avoid personal...

Page 48: ...different from the images below refer the unpacking instructions delivered with the instrument The latest unpacking instructions can also be downloaded at www qiagen com QIAcubeHT If you have any que...

Page 49: ...d at a position that is lower than the vacuum chamber so that liquids easily flow from the vacuum chamber to the vacuum control station 1 Unpack the vacuum station Note For transportation the vacuum p...

Page 50: ...ctor and vacuum chamber hoses should be stowed behind the rear cover to prevent damage To retrieve the Y hose connector of the vacuum waste hoses use one hand to reach under the cover to the left when...

Page 51: ...uum waste hose by pushing the end that does not have an attachment onto the Y hose connector 6 Plug the Polycap filter into the correct opening on the vacuum station 7 Connect the hoses between the Po...

Page 52: ...beneath the instrument 11 Connect the one end of the 15 pin accessory cable to the accessory power port on the accessory panel of the instrument see section Accessory panel and the other end into the...

Page 53: ...cket 1 Connect one end of the accessory cable to an unused accessory port on the rear panel of the instrument 1 3 in the preceding figure 2 Insert the other end through the pinch valve opening in the...

Page 54: ...fixing into the UV plug Always wear safety gloves WARNING UV irradiation hazard W9 An electronic lid sensor ensures that the instrument lid must be closed for operation of the UV lamp Under no circum...

Page 55: ...he first time ensure that the QIAcube HT Prep Manager has been installed see section Software installation Note The laptop that is supplied with the QIAcube HT supports RS 232 and USB connections We r...

Page 56: ...17 or earlier is installed on the computer refer to Upgrading from legacy QIAcube HT Software Note We recommend having no other software installed on the laptop that controls the QIAcube HT other than...

Page 57: ...te for all installation steps 11 Before the installation is completed the Device Driver Installation Wizard will open Click Next to proceed A Windows security confirmation message is displayed to indi...

Page 58: ...account with administrator privileges Note Windows administrator privileges are required for software installation only 3 Close any programs running on the computer and make sure that the QIAcube HT...

Page 59: ...Users Public QIAGEN QIAcubeHT will be removed Click OK to acknowledge the message Despite the message these items will still be available after the setup 11 If the message appeared at the beginning of...

Page 60: ...te If the computer running the QIAcube HT Prep Manager is not connected to the internet download the installation package from another computer to a USB stick Copy the installation package to the QIAc...

Page 61: ...Getting started QIAcube HT User Manual 12 2017 5 1...

Page 62: ...t to the QIAcube HT Prep Manager If the QIAcube HT is connected to the computer via USB you can switch on the QIAcube HT later after the software has already started The QIAcube HT automatically conne...

Page 63: ...Global Settings tab of the Configuration screen enables users to define general settings such as default directories e mail server details for e mail notifications printing and password options and mo...

Page 64: ...e Service environment Confirm cleaning before shut down If this option is checked the software switches to the Daily cleaning procedure wizard when the software shall be closed Security options Specif...

Page 65: ...tab of the Service environment Bar code printing Use this option if you intend to print a bar code for output labware Select the bar code type that fits your needs Select the bar code printer by clic...

Page 66: ...a run activate the Enable notifications option The actual notification options can be found in the User Settings of the individual plug ins The general network settings for the mail server and an opt...

Page 67: ...ry if you want to save the output file side by side with the experiment file Select Save to standard directory if you want to save all output files together in a defined directory See below for direct...

Page 68: ...for the file dialog that opens when you load a sample input file or an output file in the Labware Selection step Also when you scan the input or output labware bar code the software will search in th...

Page 69: ...filter during runs that use also needs to select this option Switch off after run Enabled only if the option Switch on HEPA filter during run has been selected Select Immediately to switch off the HE...

Page 70: ...the user role administrator and is sufficient to configure and operate the QIAcube HT Prep Manager For details refer to the user roles concept Note If an audit trail is required create individual use...

Page 71: ...lds Field Restriction User ID Must be unique for each user May not be empty May not contain more than 40 characters Numerical and non numerical characters are allowed Must contain at least one non num...

Page 72: ...ter use create a deactivated user profile by removing the check from this check box To activate it later follow the steps below for activating deactivating a user 6 Activate the check box in the Roles...

Page 73: ...figured password policy In case you clicked in the password field and do not want to change the password click Cancel 5 Click OK 6 The changes to the user s data are saved Deleting a user This will pe...

Page 74: ...k box to activate or deactivate a user To activate a user Add a check to the check box To deactivate a user Remove the check from the check box 5 Click OK 6 The user will be activated or deactivated a...

Page 75: ...t meet the configured password policy the New password field will remain yellow and a hint will be shown in a tooltip The Confirm new password field will remain yellow if the contents differ from the...

Page 76: ...are Manager screen contains a step marker with three steps Adapter Plates Tubes on the left The software distinguishes between the different labware types because they must be treated differently duri...

Page 77: ...er Catalog number of the labware Socket available in Adapter step only Labware suitable for a specific position on the worktable Manufacturer not available in Adapter step List of labware manufacturer...

Page 78: ...he Remove labware icon in the column of the labware you want to remove 2 A confirmation dialog will be shown the text may vary depending on whether you are removing a plate or tube 3 Click OK to perma...

Page 79: ...ration Loading worktable position A1 1 Insert the channeling block holder 2 into the left waste chamber of the vacuum chamber 1 2 Push it firmly downwards to engage the outlet into the drain The chann...

Page 80: ...QIAcube HT User Manual 12 2017 5 20 Note For worktable position A1 the calibration needs to be performed into the channeling adapter only i e without a filter plate to increase calibration precision...

Page 81: ...ard purification protocols Loading worktable position A1 1 Insert the channeling block holder 2 into the left waste chamber of the vacuum chamber 1 2 Push it firmly downwards to engage the outlet into...

Page 82: ...used positions are completely sealed 6 Load the filter plate 5 into the transfer carriage 4 Ensure that the filter plate 5 is aligned to the upper left inside the transfer carriage 4 and is completely...

Page 83: ...n of the instrument Z axis calibration defines the worktable height of each labware item to ensure precision of liquid transfers Before using any items on the worktable XY and Z axis calibration must...

Page 84: ...arts of your body are within the instrument worktable or when looking closely at calibration positions Wait until the robotic arm has completed its movements before moving any plates or troughs The ca...

Page 85: ...orm Z axis calibration manually Note QIAGEN recommends that you follow the steps from top to bottom starting with the Volume calibration and finishing with the Labware calibration step For the first c...

Page 86: ...ause all calibration information is related to an individual pipettor head In this case connect the instrument and relaunch the software 3 The Volume step is displayed by default 4 Enter the pipettor...

Page 87: ...r previous values Click the Next button to continue with Tip Rack calibration Note The Next button is available only if volume calibration data for the current pipettor head have been saved 5 7 2 Cali...

Page 88: ...possible position Z home Slow up Slow down Fast up Fast down Button Movement Slow left slow right Fast left fast right Slow forward Slow backward Fast forward Fast backward 5 7 3 Calibrate tip racks T...

Page 89: ...k Save XY Position to save the calibration data for tips at worktable position B2 8 A message pops up indicating that the pipettor will move to the home position Confirm and stand clear of the instrum...

Page 90: ...labware adapter combinations will be XY axis calibrated on the worktable positions as defined in the work list one step for each labware or labware adapter combination in a specific worktable position...

Page 91: ...brated 1 Select the desired labware from the Select labware list Only one item can be selected Note The list contains all labware that has been made available in the Labware Manager If a labware item...

Page 92: ...nally click Start calibration to start the calibration wizard Based on the work list the wizard creates the necessary XY axis and Z axis calibrations After all calibration steps have been performed al...

Page 93: ...XY calibrated only once To increase calibration precision the calibration will be performed into the channeling adapter only see Assembly for calibration Note Filter plates will not be Z calibrated t...

Page 94: ...he dialog to define the available tip positions Worktable preparation dialog Tips on 6 12 are set as available Tips at 5 are selected to refine their availability Preparing the worktable 1 Place the t...

Page 95: ...5 Selected tips are surrounded by a light blue circle column 5 Note Ensure that the visualization on the screen is identical to the actual tip loading of the tip rack on the worktable 3 Click the Loa...

Page 96: ...is step is displayed in the center The name of the labware or names of the labware adapter combination is displayed on the right side The graphic on the right shows a schematic of the labware with the...

Page 97: ...ully center in the first column of the channeling block 6 Click Save XY position to save the XY calibration position A message will be displayed and the pipettor head will move to the home position wh...

Page 98: ...the steps below 1 Place the labware and adapter listed at the top right onto the worktable position indicated in the worktable indicator For troughs ensure that troughs are placed in all positions ma...

Page 99: ...alibrated will remain in the work list allowing you to start calibration again at a later time Note Filter plates will not be Z calibrated to avoid damage of the filter Manual calibration Note We reco...

Page 100: ...p Select the desired information to be saved by activating the corresponding check boxes Option Description Volume calibration Include volume calibration data Instrument calibration Include general in...

Page 101: ...e Labware calibration data can only be imported together with volume and general instrument calibration data Important If you have already calibrated labware using this laptop before importing use thi...

Page 102: ...Operating procedures QIAcube HT User Manual 12 2017 6 1...

Page 103: ...cumstances should you place your hands under the pipetting arm when it is lowering Do not attempt to move any plates or tubes while the instrument is operating WARNING Moving parts W15 To avoid contac...

Page 104: ...template for new experiment option from the context menu icon that appears For details refer to Create an experiment from an existing experiment The new experiment will be created and displayed in the...

Page 105: ...mple type drop down menu 5 Select a pre treatment from the Pre treatment drop down menu The entries in this menu are kit dependent Refer to the kit handbook for more information 6 Select a protocol fr...

Page 106: ...re acting on your own risk if you change these options Refer to the kit handbook for information how the selected protocol makes use of the parameters Note A warning icon and a corresponding warning m...

Page 107: ...e Sample editor will be opened and the Sample ID fields will be colored yellow to indicate that this information is required 7 Enter each sample ID and optionally enter a sample description Sample nam...

Page 108: ...If the sample file contains a labware type that is different than the labware type displayed in the Input labware type drop down menu an error message is shown and the file cannot be loaded This is o...

Page 109: ...n labware barcode dialog scan the bar code and confirm by clicking OK For details refer to Scan plate barcode dialog The software automatically searches for a QDEF format file describing the output la...

Page 110: ...vailable labware of the selected category in the Labware and Labware adapter area in the middle of the screen Note Apply filter must be clicked to populate the view in the middle of the screen 7 In th...

Page 111: ...have selected an adapter from the Labware adapter area Important Troughs or plates may have slightly different dimensions despite a similar appearance Such differences can significantly affect the ca...

Page 112: ...all sample positions on the input labware Use the List tab of the input panel to view or edit the sample definition again The Graphic tab of the Output panel on the right side of the screen shows all...

Page 113: ...les 6 1 3 1 Proceed as follows 1 From the Input panel select the sample columns you want to run Note To select multiple columns either press SHIFT and left click with the mouse to select adjacent colu...

Page 114: ...FT and left clicking with the mouse to select adjacent columns or CTRL and left clicking with the mouse to select multiple nonadjacent columns 2 Click Blocked The software displays the selected column...

Page 115: ...ed in the example picture TC A1 A2 B1 B2 C1 C2 White worktable positions are not used for the run not pictured Note The run cannot be started if any worktable positions remain yellow For a step by ste...

Page 116: ...isplayed The software tracks the tip usage during operation and displays the tip availability accordingly However the software cannot detect whether tip racks have been replaced or removed in the mean...

Page 117: ...n the following chapters accordingly For the waste bottle refer to Checking the waste bottle status For the tip chute refer to Preparing tip chute and tip disposal box For worktable positions with tip...

Page 118: ...antime not using the maintenance wizard For information how to empty the waste bottle refer to chapter Emptying the waste bottle Note Click the Emptied checkbox only if you just have emptied the waste...

Page 119: ...ensure correct positioning under the tip chute Lock the tip disposal box holder with the instrument s feet on the left side of the instrument then place the tip disposal box into the tip disposal box...

Page 120: ...QIAcube HT User Manual 12 2017 6 19 Tip chute Alignment of tip disposal box holder to the instrument and the tip disposal box...

Page 121: ...bware from the worktable position Then click the Calibrate labware button The software automatically prepares a labware calibration worklist containing all labware that is required for that worktable...

Page 122: ...k box for the position If the Loaded check box is still yellow ensure that all tip positions on the graphic at the right have been set To Unavailable Required number of tips The required number of tip...

Page 123: ...single tip positions Then click on To set the selected tips to unavailable Click on Available tip positions are displayed in dark color columns 8 12 and unavailable positions are displayed in grey col...

Page 124: ...tion worklist containing all labware that is required for that worktable position for the current run and starts the labware calibration wizard Follow the instructions of the wizard For details refer...

Page 125: ...before the software will always automatically save the latest changes to the experiment when the run is started 4 Click the Start run button to start the run 5 The run will start and the progress wil...

Page 126: ...ginning of the run The intention of this step is to verify correct setup of the vacuum subsystem ensuring that the bottle and tubing are correctly connected During this test the software applies a spe...

Page 127: ...til the movement has finished When a run is paused the Pause button will change its label to Resume To resume the run 1 Ensure that the lid is closed 2 Click the Resume button 3 The Resume button will...

Page 128: ...used tips manually Vacuum performance check 6 1 5 4 If in the Setup step the optional step Vacuum performance check user interaction required has been selected a dialog box appears after defined vacu...

Page 129: ...SHIFT and left click with the mouse to select adjacent positions CTRL and left click with the mouse to select multiple nonadjacent positions or drag the mouse to select adjacent samples in a rectangle...

Page 130: ...IAgility Setup Manager software The xml file can also be used by a LIMS Laboratory Information Management System Find examples of output files in the Examples folder provided with the software C Users...

Page 131: ...cleaning procedure 2 Click Start Follow the instructions of the software For detailed information refer to Regular maintenance and Cleaning the QIAcube HT 3 After the daily cleaning procedure has bee...

Page 132: ...end of the working day Note Normally you should not switch off the instrument without closing the software If the pipettor head holds tips they will not be discarded However if it is necessary to swi...

Page 133: ...QIAcube HT Prep Manager Software QIAcube HT User Manual 12 2017 7 1...

Page 134: ...general software usage The next chapters provide an insight into the underlying concepts and the general software usage 7 1 1 Concepts The following concepts are essential for understanding how QIAcu...

Page 135: ...on to use templates The general workflow is to select a kit from the Home environment define the experiment in the Experiment environment and finally start the run It is not necessary to finish the de...

Page 136: ...ifferent file extensions The file extension for experiments is qpmx and for templates qpmt QIAcube HT Prep Manager Experiment and Template respectively Templates and experiments can be selected from t...

Page 137: ...e file The file is invalid if the checksum does not match the file s content or is missing In the event that a file is modified outside the QIAcube HT Prep Manager the file can no longer be used If th...

Page 138: ...ou in your workflow Following is an overview of the different colors used in the software and their meanings Color Description Light blue The field is interactive and clickable Dark blue The field is...

Page 139: ...ilable A step will remain yellow as long as there are missing but required entries Displaying errors and warnings 7 1 2 3 Errors and warnings are essential information that indicate a problem or an er...

Page 140: ...ta 7 1 2 4 Shortcuts The following hot keys are available in QIAcube HT Prep Manager F1 to open the help file Copy and paste operations CTRL C and CTRL V Navigation tab key cursor keys While entering...

Page 141: ...using the keyboard or by using an interactive date picker in the form of a pop up calendar A calendar icon is positioned to the right of every date field Clicking on the calendar icon opens the pop u...

Page 142: ...the up arrow to change to the next year Click the down arrow to change to the previous year Change the month day Click the left arrow to change to the previous month Click the right arrow to change t...

Page 143: ...ext Example If a cell contains Sample then the sample IDs Sample 1 Sample 2 etc are generated Selecting samples 7 1 2 6 You can use left clicking in combination with the Shift and the Control keys to...

Page 144: ...simultaneously in the Sample editor and most sample graphics samples are selected column wise An exception is the Check vacuum performance dialog where wells can be marked individually Left click on c...

Page 145: ...IAcube HT Prep Manager uses a modern task focused GUI design to facilitate the user s interaction with the application The following general user interface elements are described in this section Menu...

Page 146: ...t Open an experiment Save Save the current experiment If you have not changed the default name Experiment_MM_DD_YYYY the file selection dialog is displayed in which you can enter the new file name alo...

Page 147: ...e Edit menu you can use the undo and redo commands by clicking on the following icons in the menu View menu Option Description Go to home screen Change to the home screen Note As an alternative to usi...

Page 148: ...bar 7 1 3 2 The main toolbar contains two areas Environment icons Instrument icons You can switch to another environment by clicking the appropriate button from the main toolbar The icon of the curren...

Page 149: ...Action Instrument connected A run can be started Instrument not connected Prior to starting a run switch on the instrument Note It can take some time until the software connects to the instrument For...

Page 150: ...sk has been completed successfully If no run is in progress click the icon to switch to the maintenance schedule for details Button bar 7 1 3 3 The button bar is placed at the bottom of the screen It...

Page 151: ...log is used in various situations to open or save experiments or templates to import sample information or to save a report or an output file after a run File dialog to save an experiment Path indicat...

Page 152: ...e folder where the file is stored Use the button to navigate to the parent folder The Look in menu provides the option to select another drive by clicking the drive letter or to select one of the pare...

Page 153: ...ions described above 2 Ensure that the correct file type is selected 3 Enter the file name into the File name field 4 Click OK to save the file File extension Description qpmx Experiment qpmt Template...

Page 154: ...h is valid and can be accessed 4 Enter the file name without the file extension into the File name field 5 Select the file extension from the File type menu 6 Optionally enter a short description of t...

Page 155: ...DEF format which defines the samples for the labware It is not required that the xml file has the labware barcode as its file name The software looks into the file and compares the labware barcode wit...

Page 156: ...ents See Experiments for details Bundles tasks for maintenance system check and support of QIAcube HT See Service for details Bundles the tasks custom settings user and labware management and calibrat...

Page 157: ...dapt a saved experiment or alternatively use it to set up a new experiment that inherits the data from an old experiment For details refer to View recent experiments or Create an experiment from an ex...

Page 158: ...f the experiment name This is a visual reminder for you that there are modifications that have not yet been saved When you save the experiment the asterisk symbol disappears Clicking on the cross to t...

Page 159: ...iment If the experiment has not yet been named a dialog will be shown prompting you to enter a name for the experiment If the experiment already has a name for example if an existing experiment was op...

Page 160: ...nally HEPA filter replacement can be managed here System check Provides several self diagnostic tools Checking the connection between computer and instrument and within the instrument itself Cycling t...

Page 161: ...an instrument calibration which is essential for accurate and reliable results User Manager Manage users Create and modify users and their roles Activate and deactivate users 7 1 5 Getting help QIAcub...

Page 162: ...to the screen displayed before using the Back button Print Provides two options Print the selected topic Print the selected heading and all subtopics Select one option and confirm with OK or select Ca...

Page 163: ...r details about the template concept refer to Experiments and templates Create a template 7 2 1 1 An experiment can be saved as a template for future reuse Use this function if you plan to enter the s...

Page 164: ...s exceeded and ellipses will be shown at the end of the template name To display the full name move the mouse pointer over the experiment name and after a few seconds a tooltip will be displayed showi...

Page 165: ...the Home environment 2 The Favorite Templates list in the middle displays all favorite templates 3 Move the mouse pointer over the template name 4 The context menu icon appears 5 Click on the icon 6 T...

Page 166: ...y the full name move the mouse pointer over the experiment name and after a few seconds a tooltip will appear with the full name Opening an existing experiment 1 Click on the Home icon in the main too...

Page 167: ...table on the right displays all recent experiments 3 Move the mouse pointer over the experiment name you want to remove 4 The context menu icon is displayed 5 Click on the context menu icon to open th...

Page 168: ...n the Create Experiment list Move the mouse pointer over one of the kit entries and click on it The new experiment is created and displayed in the Experiment environment Using the File menu To create...

Page 169: ...environment favorite templates are listed in the Favorite Templates list You can either click on one of the listed templates or if the desired template is not listed click on the Create from other tem...

Page 170: ...te based experiment using the main menu select File from the main menu and click on the Create New Experiment from Template command The File Open dialog is opened allowing you to select a template Whe...

Page 171: ...isted in the Recent Experiments list Close it and start again with step 1 3 Hold the mouse pointer over the desired experiment 4 The context menu icon appears 5 Click on it 6 The context menu is opene...

Page 172: ...position on the output plate Symbol Description ok The position was processed without any exceptions warning The position was processed with exceptions that allowed further processing e g tip failure...

Page 173: ...ion on the output labware is also shown Column Description Pos output plate The position on the output labware Identifier Specifies the sample ID Flag Shows the status of the position either ok warnin...

Page 174: ...em occurred on the filter plate which impacted all output positions A1 H6 e g columns 1 6 For some exceptions the time is listed when the exception occurred if for example the lid has been opened duri...

Page 175: ...un Position Duration For an event Lists the positions that were affected by the exception First the position is listed for which the exception occurred After Destination all positions are listed that...

Page 176: ...t calibration 7 4 2 Viewing software version When you contact QIAGEN Technical Services for support purposes you may be asked to provide the software version number of your installation To see the ver...

Page 177: ...from the Filter options group box on the left to find audit trail entries that meet specific criteria Criteria you can use are specific dates date ranges or specific users All audit trail entries tha...

Page 178: ...ar for entering the dates User filter Select one of the three options to filter for all users the current user or a specific user In the latter case select a user name from the User name drop down men...

Page 179: ...Maintenance procedures QIAcube HT User Manual 12 2017 8 1...

Page 180: ...enance that is specifically described in this user manual Discard waste according to local safety regulations Switch the instrument off and disconnect the line power cord of the QIAcube HT and of the...

Page 181: ...pliChem can be used for cleaning surfaces and to soak worktable items ANIGENE formerly TriGene Medichem International can be used for soaking worktable items CAUTION Damage to the instrument C4 Do not...

Page 182: ...your local distributor for more information about flexible Service Support Agreements from QIAGEN 8 3 Regular maintenance 8 3 1 Cleaning the instrument after completing a run When the run has been com...

Page 183: ...regulations and laws 8 3 2 Cleaning the instrument before shutting down WARNING CAUTION Risk of fire W16 When cleaning QIAcube HT instruments with alcohol based disinfectant leave the instrument hoods...

Page 184: ...ape 10 Remove the tip chute and all adapters from the worktable Remove the carriage channeling adapter and riser block from the vacuum chamber Clean all parts and the reagent trough lids according to...

Page 185: ...progress 1 Click Service in the main toolbar to switch to the Service environment 2 If not yet selected click on the Cleaning procedure tab header Select Empty waste bottle 3 The waste volume as esti...

Page 186: ...y in a quaternary ammonium based solution 2 Rinse with room temperature deionized water and allow to drain and dry Greasing the O ring A tube containing vacuum grease is provided with the instrument f...

Page 187: ...fter the application of a vacuum IMPORTANT Air bleed holes should be kept clean and clear of blockages 1 To clean air bleed holes flush with a squirt bottle containing deionized water Cleaning the gui...

Page 188: ...ment lid must be closed for operation of the UV lamp Under no circumstances should this lid sensor be disabled If the lid sensor is not functioning correctly contact QIAGEN Technical Services To use t...

Page 189: ...ce Specialist or your local distributor for more information We recommend regular servicing of the pipettor The recommendation will be displayed by the maintenance schedule As P axis movements and the...

Page 190: ...ou an overview of scheduled upcoming service tasks and recent tasks You can find this tool in the Regular Schedule step of the Maintenance tab in the Service environment Scheduled tasks upper table an...

Page 191: ...us Current status of the task The cell is empty if the task is not due If the task is due the reason why the task is due will be displayed because the service period has been exceeded or if the number...

Page 192: ...QIAcube HT User Manual 12 2017 8 14 The View icon has the identical functionality in both tables it opens the View service task dialog with detailed information...

Page 193: ...HT pipettor QIAGEN recommends that a regular preventive maintenance service is carried out on the QIAcube HT The service should be performed by a trained QIAGEN Field Service Specialist Contact your...

Page 194: ...lters 1 Click the Service button in the main toolbar to switch to the Service environment Click on the Maintenance tab and then click on the Replace HEPA filter step Note The step is available for use...

Page 195: ...lly acknowledges the task as successfully completed The task will be listed in the Last service tasks table of the Regular Schedule In the task details the task will be marked as successfully complete...

Page 196: ...are provides a step by step wizard to guide you through all steps to replace the vacuum filter The wizard can be accessed by users with user role Administrator via the Replace vacuum filter step in th...

Page 197: ...filter step Note The step is available for users with user role Administrator only 2 Click Start to start the procedure now The next step will be displayed 3 If the instrument is still switched on you...

Page 198: ...ully completed The task will be listed in the Last service tasks table of the Regular Schedule In the task details the task will be marked as successfully completed Note If you leave the wizard by cli...

Page 199: ...Troubleshooting QIAcube HT User Manual 12 2017 9 1...

Page 200: ...es This will help the QIAGEN Field Service Specialist resolve the issue It is also possible to export support packages even if the instrument is powered down A warning will be issued when an error suc...

Page 201: ...to return to the message dialog 9 1 Support packages Support packages are folders containing multiple files that help QIAGEN Technical Services to analyze issues that might have been observed The fun...

Page 202: ...lts of performed system checks Instrument issues Note Support packages older than 90 days will be automatically deleted Exporting a support package 1 Click on the Support Package tab in the Service en...

Page 203: ...ted directory and send all files in it to QIAGEN Technical Services The directory contains the support packages of the selected experiments and or system test results including plug in specific files...

Page 204: ...initialize on software start It initializes just before use e g before calibration or during worktable planning before a run Computer software cannot communicate with QIAcube HT a USB or RS 232 cable...

Page 205: ...stalled click the Windows Start icon at the bottom left of the Windows screen Then right click on the entry Computer in the right bar and select Properties from the appearing context menu Check that t...

Page 206: ...ext of an installation of the QIAcube HT Prep Manager allow to install the driver anyway If you do not install the driver software the QIAcube HT Prep Manager a will not be able to operate the QIAcube...

Page 207: ...rs and power save modes not disabled on the computer Disable screen savers and power save modes on the computer Vacuum errors a Vacuum could not be established 1 Adapters and labware used in vacuum ch...

Page 208: ...via RS 232 cable the software needs to be restarted in order to connect to the instrument b Mandatory calibrations missing During run setup the worktable step can only be accessed if the mandatory ca...

Page 209: ...nitial step shows a description of the check its duration and a Start button Click the Start button to begin the check and to display the instructions for the next step Follow the instructions on the...

Page 210: ...es and contact QIAGEN Technical Services for further assistance Click Restart if you want to repeat the task Click on another step marker to perform another test Or click to another part in the softwa...

Page 211: ...pettor head 4 Close the lid 5 Click Done to confirm and proceed 6 Wait while the instrument moves the robotic arm to check for home opto alignment 7 If the test finished the software displays the test...

Page 212: ...ted packages and contact QIAGEN Technical Services for further assistance Click Restart if you want to repeat the task Click on another step marker to perform another test or click to another part in...

Page 213: ...Glossary QIAcube HT User Manual 12 2017 10 1...

Page 214: ...values must be entered when configuring QIAcube HT Channeling adapter Adapter that is part of the Accessories pack The channeling adapter consists of the channeling block holder and the channeling blo...

Page 215: ...ed on the instrument based on a kit For each kit to be used on QIAcube HT one kit configuration is available L Labware library The labware library is delivered with the software and contains all labwa...

Page 216: ...spensed to reaction wells that might contain samples A reagent can be combined with other reagents or added individually to a reaction Reagent plate Special adaptor that can be placed on position R1 T...

Page 217: ...vacuum chamber consists of a waste and an elution chamber that are both connected via hoses to the vacuum control station Vacuum station The vacuum station is part of the QIAcube HT It contains the v...

Page 218: ...QIAcube HT User Manual 12 2017 10 6...

Page 219: ...Appendices QIAcube HT User Manual 12 2017 11 1...

Page 220: ...0 of the nominal supply voltages Fuse T5A 250V instrument F4A 250V vacuum pump Overvoltage category II Air temperature 15 30 C 59 86 F Relative humidity 40 70 Altitude Up to 2000 m 6500 ft Place of op...

Page 221: ...waste it must be taken to an approved treatment facility or to a designated collection point for recycling according to local laws and regulations The separate collection and recycling of waste electr...

Page 222: ...ts for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commerc...

Page 223: ...roved by an officer of QIAGEN 11 3 Appendix C Consignes de s curit Avant d utiliser le QIAcube HT il est imp ratif de lire attentivement ce manuel et de porter une attention particuli re aux consignes...

Page 224: ...une bonne ventilation laisser un d gagement d au moins 10 cm sur les c t s et l arri re du QIAcube HT Les fentes et les ouvertures qui assurent la ventilation de l appareil ne doivent pas tre obstru e...

Page 225: ...la lumi re directe du soleil ATTENTION D t rioration de l appareil C2 viter de renverser de l eau ou des produits chimiques sur le QIAcube HT La d t rioration de l appareil d au d versement de liquid...

Page 226: ...cas tre d sactiv Si le capteur de couvercle ne fonctionne pas correctement prendre contact avec les services techniques de QIAGEN Afin que le QIAcube HT fonctionne de mani re satisfaisante et en toute...

Page 227: ...s des lunettes de protection deux paires de gants et une blouse de laboratoire La personne responsable par exemple le directeur du laboratoire doit prendre les pr cautions n cessaires afin de garantir...

Page 228: ...onales r gionales et locales relatives la sant et la s curit OSHA Occupational Safety and Health Administration tats Unis d Am rique Administration pour la sant et la s curit du travail ACGIH American...

Page 229: ...acement manuel accidentel du bras Y il est fortement recommand de red marrer le syst me Afin que le QIAcube HT fonctionne de mani re satisfaisante et en toute s curit suivre ces instructions Utiliser...

Page 230: ...e dans ce manuel ATTENTION D t rioration de l appareil C4 Ne pas utiliser de solvants ni de r actifs contenant des acides des bases ou des compos s abrasifs pour nettoyer le QIAcube HT ATTENTION D t r...

Page 231: ...n des vapeurs inflammables ATTENTION D t rioration du chariot de transfert de la plaque de capture C7 En aucun cas le chariot de transfert de la plaque de capture ne doit tre autoclav ATTENTION R inst...

Page 232: ...Label CSA pour le Canada et les tats Unis Plaque signal tique l arri re de l appareil Fabricant l gal Plaque signal tique l arri re de l appareil Symbole DEEE Plaque signal tique l arri re de l appar...

Page 233: ...n k nnten N here Einzelheiten ber diese Situationen werden in einem Textfeld wie diesem beschrieben Die in diesem Handbuch enthaltenen Hinweise stellen eine Erg nzung und keinen Ersatz der blichen Sic...

Page 234: ...mosph ren vorgesehen WARNUNG ACHTUNG Explosionsgefahr W6 Der QIAcube HT ist ausschlie lich mit Reagenzien und Substanzen aus den QIAGEN Kits zu benutzen Die Benutzung von anderen Reagenzien oder Subst...

Page 235: ...ist sind die Anschlussstellen spannungsf hrend Durch das ffnen der Abdeckungen oder das Entfernen von Geh useteilen k nnen spannungsf hrende Komponenten freigelegt werden WARNUNG Gefahr durch UV Stra...

Page 236: ...ige Proben die mithilfe dieses Ger ts verarbeitet werden k nnen infekti se Erreger enthalten Gehen Sie beim Umgang mit diesen Proben mit der gr tm glichen Vorsicht und gem den erforderlichen Sicherhei...

Page 237: ...r einem funktionierenden Laborabzugssystem durchgef hrt werden damit die m glicherweise entstehenden D mpfe abziehen k nnen 11 4 5 Abfallentsorgung Benutzte Kunststoff Laborartikel k nnen gef hrliche...

Page 238: ...ie bis die Bewegungsabl ufe des Roboterarms beendet sind bevor Sie Reaktionsgef e oder Platten bewegen Stellen Sie keine Gegenst nde auf den Roboterarm W hlen Sie w hrend eines Laufs die Schaltfl che...

Page 239: ...ien die S uren Laugen oder Abrasivstoffe enthalten um die QIAcube HT Haube zu reinigen Alkohol sch digt die Ger tehaube Verwenden Sie zum Reinigen der Ger tehaube ausschlie lich destilliertes Wasser A...

Page 240: ...echsel des QIAcube HT C10 Der wichtigste Bestandteil der Verpackung ist die Holzst tze die den Y Arm fixiert Diese St tze muss verwendet werden wenn der QIAcube HT an einen anderen Standort verlagert...

Page 241: ...seite des Instruments Hersteller i S d Gesetzes Typenschild auf der R ckseite des Instruments WEEE Kennzeichnung gem europ ischer Richtlinien bzw Elektround Elektronik Altger te Verordnung Typenschild...

Page 242: ...10 plus lid liquid reservoirs used with Reagent Trough R1 Position Plate and Reagent Trough SBS Plate 990554 Adapter Reagent Trough SBS Plate For holding combinations of 70 ml and 170 ml Reagent Trou...

Page 243: ...cartridge one filter 9019483 Tip Disposal Box Box of 10 waste collection box to fit tip ejector chute 990550 Tip Disposal Box Holder Holds the tip disposal box in place and ensures correct positionin...

Page 244: ...THE LOCATION FROM WHICH IT WAS OBTAINED FOR A FULL REFUND OF THE AMOUNT YOU ORIGINALLY PAID This License Agreement Agreement is a legal agreement between Xceed Software Inc Xceed a Quebec corporation...

Page 245: ...es that are Authorized and 2 software to be licensed or distributed under an open source model including without limitation models similar to GNU s General Public License GPL Lesser GPL the Artistic L...

Page 246: ...y any means by which end users could incorporate the Software or portions thereof into their own products ii not use Xceed s name logo or trademarks to market a software product iii include a valid co...

Page 247: ...ding the distribution of Redistributable Files and vi Licensee will not request technical support or error corrections from Xceed on issues arising out of any modifications of the Source Code 5 SAMPLE...

Page 248: ...Xceed within 30 days of the transfer Upon transferring the Software to another party Licensee must terminate this Agreement by following the instructions in the AGREEMENT TERMS section below 8 REVERS...

Page 249: ...s and controls Licensee agrees and certifies that neither the Software nor any direct product thereof e g any application software product developed by Licensee that uses the Software is being or will...

Page 250: ...s agree that federal and provincial courts located in Longueuil Quebec will have exclusive jurisdiction and that a suit may only be brought in Longueuil Quebec and Licensee submits itself for the juri...

Page 251: ...transfer of data 1 5 Executable means Covered Code in any form other than Source Code 1 6 Initial Developer means the individual or entity identified as the Initial Developer in the Source Code notice...

Page 252: ...ded the appropriate decompression or de archiving software is widely available for no charge 1 12 You or Your means an individual or a legal entity exercising rights under and complying with all of th...

Page 253: ...er on an unmodified basis with other Modifications as Covered Code and or as part of a Larger Work and b under Patent Claims infringed by the making using or selling of Modifications made by that Cont...

Page 254: ...ilable for at least twelve 12 months after the date it initially became available or at least six 6 months after a subsequent version of that particular Modification has been made available to such re...

Page 255: ...in a location such as a relevant directory where a user would be likely to look for such a notice If You created one or more Modification s You may add your name as a Contributor to the notice descri...

Page 256: ...Works You may create a Larger Work by combining Covered Code with other code not governed by the terms of this License and distribute the Larger Work as a single product In such a case You must make...

Page 257: ...from the Mozilla Public License and Netscape Public License Filling in the name of the Initial Developer Original Code or Contributor in the notice described in Exhibit A shall not of themselves be de...

Page 258: ...iting by the parties or the litigation claim is not withdrawn the rights granted by Participant to You under Sections 2 1 and or 2 2 automatically terminate at the expiration of the 60 day notice peri...

Page 259: ...in 48 C F R 12 212 Sept 1995 Consistent with 48 C F R 12 212 and 48 C F R 227 7202 1 through 227 7202 4 June 1995 all U S Government End Users acquire Covered Code with only those rights set forth he...

Page 260: ...obtain a copy of the License at http www mozilla org MPL Software distributed under the License is distributed on an AS IS basis WITHOUT WARRANTY OF ANY KIND either express or implied See the License...

Page 261: ...license governs use of the accompanying software If you use the software you accept this license If you do not accept the license do not use the software 1 Definitions The terms reproduce reproduction...

Page 262: ...any portion of the software you must retain all copyright patent trademark and attribution notices that are present in the software D If you distribute any portion of the software in source code form...

Page 263: ...re components that are incorporated in installer packages generated by WiX can be governed by other licensing terms The full text of the MS RL license is reproduced below It can also be found in the L...

Page 264: ...are present in the software E If you distribute any portion of the software in source code form you may do so only under this license by including a complete copy of this license with your distributio...

Page 265: ...the first released version of the Lesser GPL It also counts as the successor of the GNU Library Public License version 2 hence the version number 2 1 Preamble The licenses for most software are desig...

Page 266: ...the users of a free program by obtaining a restrictive license from a patent holder Therefore we insist that any patent license obtained for a version of the library must be consistent with the full...

Page 267: ...or other authorized party saying it may be distributed under the terms of this Lesser General Public License also called this License Each licensee is addressed as you A library means a collection of...

Page 268: ...at in the event an application does not supply such function or table the facility still operates and performs whatever part of its purpose remains meaningful For example a function in a library to co...

Page 269: ...ce code even though third parties are not compelled to copy the source along with the object code 5 A program that contains no derivative of any portion of the Library but is designed to work with the...

Page 270: ...s files in the Library will not necessarily be able to recompile the application to use the modified definitions b Use a suitable shared library mechanism for linking with the Library A suitable mecha...

Page 271: ...the Library or its derivative works These actions are prohibited by law if you do not accept this License Therefore by modifying or distributing the Library or any work based on the Library you indica...

Page 272: ...ons will be similar in spirit to the present version but may differ in detail to address new problems or concerns Each version is given a distinguishing version number If the Library specifies a versi...

Page 273: ...at was posted by JasonSmith 12 13 2 Jan 04 Feel free to use this code any way you want to As a favor to me you can leave the copyright in there You never know when someone might recognize your name If...

Page 274: ...mechanical transformation or translation of a Source form including but not limited to compiled object code generated documentation and conversions to other media types Work shall mean the work of au...

Page 275: ...the Work and such Derivative Works in Source or Object form 3 Grant of Patent License Subject to the terms and conditions of this License each Contributor hereby grants to You a perpetual worldwide n...

Page 276: ...ative Works if and wherever such third party notices normally appear The contents of the NOTICE file are for informational purposes only and do not modify the License You may add Your own attribution...

Page 277: ...ligent acts or agreed to in writing shall any Contributor be liable to You for damages including any direct indirect special incidental or consequential damages of any character arising as a result of...

Page 278: ...ware on your devices 2 ADDITIONAL LICENSING REQUIREMENTS AND OR USE RIGHTS a Distributable Code You are permitted to distribute the software in programs you develop if you comply with the terms below...

Page 279: ...ns in the software reverse engineer decompile or disassemble the software except and only to the extent that applicable law expressly permits despite this limitation make more copies of the software t...

Page 280: ...ountry if the laws of your country do not permit it to do so 12 DISCLAIMER OF WARRANTY THE SOFTWARE IS LICENSED AS IS YOU BEAR THE RISK OF USING IT MICROSOFT GIVES NO EXPRESS WARRANTIES GUARANTEES OR...

Page 281: ...tes Inc Gigasept Lysetol Mikrozid Sch lke Mayr GmbH Microsoft Windows Microsoft Corporation Teflon E I du Pont de Nemours and Company Adobe Reader Adobe Systems Inc Registered names trademarks etc use...

Page 282: ...1109028 12 2017 HB 2120 003 www qiagen com Technical Support www support qiagen com...

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