QIAcube Connect MDx User Manual 12/2020
22
mode of the software does not support this feature. Instead, the user needs to be in front of the
instrument and use the touchscreen of the instrument to set up a run.
3.1
QIAcube Connect MDx principle
Sample preparation using the QIAcube Connect MDx follows the same steps as the manual
procedure (i.e., lyse, bind, wash and elute). Depending on chosen application, the procedure may
be alternated, or steps may not be required. Moving forward to laboratory automation, no change
in purification chemistry is required as you simply continue to use trusted QIAGEN spin-column kits.
1.
Samples are lysed in the orbital shaker, which can be heated if required by the protocol.
2.
Each lysate is transferred to a spin column in a rotor adapter. If the lysate needs to be
homogenized or cleared, it is first transferred to the middle position of the rotor adapter.
3.
Nucleic acids or proteins bind to the silica membrane or purification resin of the QIAGEN
spin column and are washed to remove contaminants.
4.
The spin column is transferred to a microcentrifuge tube for elution of purified nucleic acids or
protein.
For an example workflow using a QIAGEN spin-column kit, see the QIAamp DNA DSP Procedure
flowchart on the next page.
