Qiagen PyroMark Q24 MDx, User Manual

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Troubleshooting
7-2 PyroMark Q24 MDx User Manual 01/2016
runs, please contact QIAGEN Technical Services. If
requested to send an Environment Data file:

Select “Export Environment Data” from the “Tools”
menu

Select the destination folder for the data file from the
“Save in” drop-down list

Enter the filename in the “File name” text box and
click “Save”
7.1 Analysis-related errors
Comments and suggestions
a) PCR failed due to
low DNA quality
Check the PCR samples using an agarose gel to
confirm there is one strong specific band. If not,
rerun PCR with high-quality DNA.
The PyroMark PCR Kit is recommended for highly
specific amplification of bisulfite-converted DNA
and genomic DNA from various sources.
b) Poorly optimized
PCR
Check the PCR samples using an agarose gel to
confirm there is one strong specific band. If not,
reoptimize PCR.
c) Biotinylation is
omitted or not
added to the correct
PCR primer
Check assay design; see Appendix B.
d) Biotinylation is of
poor quality
Use a recommended primer supplier. Ensure the
biotinylated primer is HPLC-purified or similar.
e) Insufficient amount
of template for
immobilization to
Sepharose beads
Follow the recommendations for amount of
template; see Appendix B.