PSS magLEAD 5bl User Manual Download

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Fully Automated Nucleic Acid Purification System

User’s Manual

Ver.

1.07

Summary of Contents for magLEAD 5bl

Page 1: ...Fully Automated Nucleic Acid Purification System User s Manual Ver 1 07...

Page 2: ...2 For Safety Usage 4 3 Specifications 9 4 System Components 16 5 Installation 21 6 Accessories and Disposables 23 7 Direction for Use 26 8 Basic Operations 31 9 Maintenance 37 10 Troubleshooting 39 1...

Page 3: ...s quick reference for all the operators as needed Do not operate the instrument without sufficiently understanding instructions described in this book Always keep this book nearby for easy reference a...

Page 4: ...instrument The explanation of each symbol is described below This indicates the possibility that one could be killed or seriously wounded when inadequate operation is done This indicates the possibil...

Page 5: ...estrict operations or to illustrate what to do Details are illustrated inside the symbol the sample on the left shows unplugging of AC Plug from wall outlet The following precautionary instructions ar...

Page 6: ...such as extraordinary hot smoking abnormal smell etc immediately unplug power cable from wall outlet to avoid fire or electric shock to you and contact with us for repair When foreign material get ins...

Page 7: ...nt shall be made by field service personnel authorized by PSS Contact us when you transfer the instrument Storage Unplug power cable from wall outlet when the instrument may not be used for extended p...

Page 8: ...us for fuse replacement After usage of instrument or if any sample or reagent leaked inside the instrument remove Reagent Cartridge Rack and Combo Tube Rack to make a cleanup and sterilization About W...

Page 9: ...icians trained in molecular biological techniques and the operation of the magLEAD 5bL 3 2 Features magLEAD 5bL instrument makes the pretreatment of samples such as genetic screening the main purposes...

Page 10: ...h distilled water at room temperature 20 25 deg C Note Processing Time depends on protocols 3 4 Size Weight and Power Requirement Size H528 x W440 x D664 mm Disregarding prongs Weight 40kg Excluding a...

Page 11: ...15 75 Non Condensing Barometric Pressure More than 70kPa min 3 7 Environmental Requirement Overvoltage Category Pollution Degree 2 Installation Indoor Site Use Only Temperature 15 30 deg C Relative H...

Page 12: ...place the instrument where it will get direct sunlight or vibration When operating instrument in a cold chamber or cold room keep the instrument power on to avoid bedewing 3 8 About Caution Labels Ins...

Page 13: ...ty of biohazard contamination with samples or extracted purified products Take care not to be contaminated Note that it is not possible to completely eliminate risk of biohazard from samples or extrac...

Page 14: ...gnostic IVD medical equipment IEC61326 1 2013 Electrical equipment for measurement Control and laboratory use EMC requirements Part1 General requirements IEC61326 2 6 2013 Electrical equipment for mea...

Page 15: ...15 In Vitro Diagnostic medical devices 98 79 EC magLEAD 5bL is not considered as a medical device IVD in the United States of America and it is not registered with the FDA...

Page 16: ...16 4 System Components 4 1 Front View 1 Light when powered on green light Blinks when an error occurred red light Working orange light Operation Panel Power Lamp 1 Front Door LCD Panel SD card slot...

Page 17: ...ht key Operation of this key will move a cursor right Lower key Operation of this key will move a cursor downward Enter key Operation of this key will start the protocol or be settled action items 4 2...

Page 18: ...the back side of instrument 4 3 1 Back View 4 3 2 Back bottom view Do not connect any device other than devices authorized in the operating manual for options to RS 232C port USB Port For maintenance...

Page 19: ...zed by PSS Contact us for fuse replacement 4 4 Internal Components Inside the instrument Magtration Unit and Stage Unit are located 4 4 1 Magtration Unit Bellows Holder BT 20 tips up to 5 samples can...

Page 20: ...and Reagents Cartridge can be loaded Heat Block highlighted with red line Combo Tube Rack Sample and Elution Tubes can be loaded UV Lunp Sterilizes inside the instrument after Reagent Cartridge Rack i...

Page 21: ...o fix movable parts Remove the cushioning Connect Power Cable to Power Inlet Connector on the back of the instrument Then turn Power Switch on assuring that Front Door is closed securely Do not use po...

Page 22: ...22 Setting of the instrument shall be made by field service personnel authorized by PSS Contact us when you transfer the instrument Warning...

Page 23: ...er cable 1 6 1 1 Reagent Cartridge Rack It holds up to five Lx Cartridges 6 1 2 Combo Tube Rack It holds up to five Tubes of 50 0ml and 2 0ml 6 1 3 SD Card It is used for update of firmware and protoc...

Page 24: ...information of Reagents and Tips 6 2 Disposables 6 2 1 Disposables Produced by PSS List of Disposables Produced by PSS Code Product Description Place to be Used F7230 magLEAD Tip LV set BT Holder F13...

Page 25: ...sted above may be recommended in some applications Refer to Application Manual for details 6 3 Reagents Refer to each Application Manual for reagents to be used and directions for use Do not reuse any...

Page 26: ...it is not possible to resume from where it stopped If you need to open or close the door while running stop protocols or working and then open close the door after the instrument stops completely If...

Page 27: ...ve force to avoid damaging it 7 3 Setting Reagent Cartridges and Disposables 7 3 1 Setting Reagent Cartridges on Reagent Cartridge Rack Orient each Reagent Lx Cartridge with the arrow pointing towards...

Page 28: ...ng them in Combo Rack 7 3 3 Setting Combo Tube Rack Set combo tube rack into the instrument Flat end of the rack in back side and the other end of the rack in front Instrument operation without correc...

Page 29: ...bellows tip holder so that the flange highlighted by red arrow is placed under the bellows Set all the tips and slide Bellows Lock to left to lock Instrument operation without correct placement of Be...

Page 30: ...nstrument malfunction Be sure to wear a mask goggles and gloves for your own safety when loading or discarding reagents consumables and samples When discarding consumables follows the instructions def...

Page 31: ...f it is in operation press Protocol Stop button and make all axes return to ORG position to stop the operation 8 2 How to run the Protocol program Please set reagents and consumables in the instrument...

Page 32: ...od of operation depends on the reagent Please refer to the instruction manual for the respective reagents 8 3 Menu for Manual operation This screen is for the purpose of manual axis movement and maint...

Page 33: ...anual movement with key on Manual operations screen and press key to execute Select axis with key on Manual movement screen and press key to execute Select movement by key ORG Return to home position...

Page 34: ...ncel and key to execute OK to start UV exposure and countdown the time Cancel to return back to manual operation menu UV Decontamination in process Automatically return to top menu when completed Ligh...

Page 35: ...til it stops at the limit sensor displayed the stopped position coordinates Each axis can be moved manually Temperature The temperature of the heat block can be set as desired display of current tempe...

Page 36: ...o select the set item key to execute and key to return to previous menu Protocol Add Delete To add and delete the Protocol Please update along with the software update documentation Date Cur CMM CD CY...

Page 37: ...the instrument for your safety according to the description in this book Maintenance work shall be made with instrument power off Pay close attention when cleaning places where caution labels are atta...

Page 38: ...e using paper such as Kim Wipe moistened with the liquid Use a mask goggles and gloves when cleaning the instrument for your safety Water 70 Ethanol Note Do not use alcohol when cleaning LCD Panel or...

Page 39: ...you make an inquiry for an error the error code is also necessary Write down the code somewhere The protocol run aborted by an error cannot be restarted from the point of abortion Note Repeat the same...

Page 40: ...be taken up If Front Door is opened or closed during Pause and any axis is moved manually click Break to return to Top Menu and set necessary disposables on the stage again If resumed to a protocol r...

Page 41: ...started press STOP to abort instrument operation It is strongly recommended to restart the protocol run from the beginning after completion of adding disposables If you want to continue the protocol...

Page 42: ...the origin of M axis 15 While in run fail to return to the origin of Z axis 16 While in run Z axis end limit error occurs 17 While in run P axis end limit error occurs 18 While in run M axis end limi...

Page 43: ...e related to system software or protocols Take note of the error code and contact PSS for further technical support 111 Overflow of script buffer 112 Overflow of script buffer pointer 113 Overflow of...

Page 44: ...r string N A 150 Incorrect parentheses 151 Incorrect large parentheses N A 160 Inconsistence with variable table N A 170 The assigned variable is already registered in variable table 171 Incorrect var...

Page 45: ...ta 234 Number of character of header data in one row to be read overflows 20 characters NA 240 Assigned function has already been registered in function table 241 Incorrect function name NA 250 Incorr...

Page 46: ...er technical support 310 Failure of jump address search in a protocol 311 Incorrect wait time set in a protocol 312 Layer of function call exceeds limit 313 Failure of InputStr in a protocol NA 320 Do...

Page 47: ...described contact us with recording the error codes Note Specifications and appearance of the product may be changed without prior notification Manufacturer Precision System Science Co Ltd Main Offic...

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