ProteOn XPR36, User Manual

Page: 87 / 288

Preparing the Samples
69
To set up a sample holder:
1. Place the vials in the appropriate sample holder, according to the
Protocol Samples screen placement. See Sample Panel Browser on
page 45. See also Sample Holders on page 203 for an illustration of
microplates and sample racks.
2. With a pipet, fill the sample vials to the appropriate level.
3. Place pierceable caps on the vials. Injection delays due to bubbles in
one or more channels can be due to a vacuum when using 1.5 ml caps.
4. To avoid vacuum formation, pre-pierce the vials off-center with a sharp
needle, allowing ventilation during sample aspiration. If you are using a
microplate, cover the samples with microplate sealing film.
5. Place the sample holder on the thermal platform inside the autosampler
compartment.
6. Close the autosampler compartment door.
Setting the Autosampler Temperature
The autosampler rack and microplate holder is a thermal platform that can
have its temperature adjusted at any time on the Instrument Control screen.
The current temperature is displayed both on the status bar and in the
Autosampler box on the Instrument screen.
The temperature may be set at any point in a range from 2–35°C.
To set the autosampler temperature:
1. Select the Instrument Control screen in the Instrument tab.
Warning! You must use the needle holder
corresponding to the type of sample holder you
are using. Failure to do so may damage needles.
See Sample Holder Sensors on page 204.