ProteOn XPR36, User Manual

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Creating and Editing Protocol Steps
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The following step types can be dragged from the Step list.
C
OPYING AND
P
ASTING
P
ROTOCOL
S
TEPS
Two Paste options are available. The Paste option makes a new step using all
the parameters of the object copied but not the sample location. The Paste
Replicate option copies all the parameters of the object copied including the
sample location.
When you select Paste for any step that is not Blank or Regenerate, a new
sample panel is used each time you paste. When you create a Blank or
Regenerate step, the system uses the same sample well until it is empty for
both the Paste and Paste Replicate options.
Step Type Description
Activate Activation of the sensor chip surface (for amine coupling GLC, GLM, and
GLH sensor chips only)
Ligand Control of ligand flow across the sensor chip for immobilization
Deactivate Deactivation of the sensor chip surface (in amine coupling GLC, GLM, and
GLH sensor chips only)
Regenerate Regeneration of the sensor chip surface
Analyte Control of analyte flow across the sensor chip for interaction analysis
Blank Control of buffer injections across the sensor chip for chip referencing
and equilibration
CoInject Ligand Sequential injections of ligand
CoInject Analyte Sequential injections of analytes
Pause Pause protocol execution. A pause step can be added to a protocol
manually or automatically (to allow wait time for baseline stabilization, for
instance).
Note: In this example, the pause is added manually. An example of an
automatic pause is a step placed into the protocol to allow time to change
the rack or microplate (noninjection step)
Set Temperature Changes to chip/flow cell temperature in the instrument and recalculates
the SPR reference
Set Buffer Specifies which of the two running buffers to use for subsequent steps
(noninjection step) and recalculates the SPR reference
Change Rack/
Plate Allows switching between sample holders at any point in a protocol
(noninjection step)