ProteOn XPR36, User Manual

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ProteOn XPR36 System
247
Glossary
4PL Four-parameter logistic curve, for which the mathematical formula is:
Slope (RU/s) is the derivative of the response unit by time
R
lo
is the estimated response at zero concentration
R
hi
is the estimated response at infinite concentration
A1 is the mid-range concentration
A2 is the slope factor
A1 Mid-range concentration
A2 Slope factor
Activation Creation of a chemically active sensor chip surface for covalent
immobilization of ligands. For the ProteOn™ GLC and GLM sensor
chips, activation typically involves treatment of the chip surface with
amine coupling reagents such as 1-ethyl-3-(3-dimethylaminopropyl)
carbodiimide hydrochloride (EDAC) and N-hydroxysulfosuccinimide
(Sulfo-NHS)
Affinity Measurement of the strength of a binding interaction—the attractive
force between substances, particles, or interactants (for example,
biomolecules) that causes them to form a complex (see
Avidity )
Analyte Chemical substance that is the subject of analysis. In the ProteOn™
XPR36 protein interaction array system, the analyte is the interaction
partner that flows over the immobilized ligand
Area of Interest See Interaction spot
Association Aggregation or binding of molecules to form complexes
Association Phase Interval during which the analyte is injected over the ligand surface
Association Rate
Constant Also known as kinetic rate constant (k
a
), the “on rate,” describing the
rate of association of interactants. It is expressed in M
-1
sec
-1
units.
Typical values for the working range are 3 x 10
3
to 3 x 10
6
M
-1
sec
-1