ProteOn XPR36, User Manual

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ProteOn XPR36
225
Appendix
D Protocol Development
Kits
ProteOn™ protocol development kits are designed for use with the ProteOn™
XPR36 protein interaction array system. Each kit provides the reagents and
sensor chip needed to complete an interaction analysis experiment.
One-shot Kinetics™ (OSK)™ Kit
The ProteOn One-shot Kinetics™ kit provides the materials for analyzing the
interaction between the cytokine IL-2 and an antibody to IL-2. This kit uses
the interaction between the two proteins to demonstrate a detailed kinetic
analysis in a single injection cycle without regeneration between samples. In
addition, the kit demonstrates a rapid and efficient method for controlling
ligand immobilization levels for use in protocol development and optimization,
made possible by the parallel processing capability of the ProteOn XPR36
system.
Using this kit, the ligand (antibody to IL-2) is immobilized at five different levels
on a sensor chip. The immobilization level depends on the degree of surface
activation. This process is followed by injection of an analyte (IL-2)
concentration series in six orthogonal channels. Each interaction produces a
set of sensorgrams suitable for detailed kinetic analysis.
Multiple Protein Interaction Kit
The ProteOn multiple protein interaction kit provides the materials for
analyzing the interaction between TEM1
β
-lactamase and
β
-lactamase
inhibitor protein. This kit uses the interaction between the two proteins to
produce a detailed kinetic analysis of multiple simultaneous interactions and
to map protein interfaces.
The kit contains five different mutant-derived forms of the TEM1
β
-lactamase
protein along with the wild-type
β
-lactamase inhibitor protein (BLIP). The five
TEM1 mutated proteins are bound to a sensor chip in five parallel channels. A
BLIP dilution series is then injected into six orthogonal channels to create the
interaction array. Five sets of six sensorgrams are produced simultaneously,
enabling rapid comparison of the binding kinetics for each TEM1 mutant.