ProteOn XPR36, User Manual

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ProteOn XPR36 | B Specifications and Requirements
210
Working Ranges (Typical Values)
• Association rate constant (k
a
): 3 x 10E
3
to 3 x 10E
6
M
-1
s
-1
• Dissociation rate constant (k
d
): 5 x 10E
-5
to 6 x 10E
-1
s
-1
• Equilibrium dissociation constant (K
D
): 2 x 10E
-4
to 1.6 x 10E
-11
M
Fluidics
• Autosampler: Thermal-controlled sample rack holds 72 (12 x 6
format) 2 ml sample vials, or two 96-well (8 x 12 format)
microplates. Temperature-controlled in the range of 2
– 35ºC
• MCM (multichannel module): 6 parallel fluid paths, 450 μm wide x
100 μm high
• Syringe pumps: 6 sample and 6 buffer syringe pumps
• Syringe pump volume: 500 μl per stroke
• Sample injection volume: 25 – 449 μl
• Required sample volume:
m 25 μl Minimum injected volume
+ 35 μl System dead volume
+ 25 μl Vial dead volume
+
n 8 μl x Number of bubbles used as separators
= 93 μl Minimum amount of sample in a vial
• Sample vial dead volume: 25 μl
• Sample flow rate: 25 – 200 μl/min
• Sample flow rate uniformity in 6 parallel channels: 99% (CV <1%)
• Online degasser: For the buffer system only
• Buffer valves: Switch between two different running buffers
Physical
• Dimensions (W x D x H): 99 x 66 x 58 cm (39 x 26 x 23 in)
• Weight: 85 kg (187 lb)
• Power requirements: 100 – 240 V, universal (maximum power
consumption 800 W)
• Equipment intended for indoor use
• Elevation up to 2,000 m
• Ambient temperature range: 18 – 30ºC
• Ambient humidity range: 20 – 70% RH
• Mains supply fluctuations of ± 10%
• Overvoltage category II of IEC 60364-4-443
• Pollution degree II