ProteOn XPR36, User Manual

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ProteOn XPR36
209
Appendix
B Specifications and
Requirements
System Specifications
Detection System
• Number of interaction spots: 36
• Interaction spot dimension: 0.2025 mm
2
• Number of interspot references: 42
• Uniformity of response: >98% (CV <2%)
• Refractive index range: 1.33 – 1.37 refractive index units (RIU)
• Dynamic range: 1 – 40,000 response units (RU)
• ProteOn XPR36 system is verified to have 1 RU set = 1 x 10
-6
RIU
• Baseline noise:
<1 RU, 1 – 20,000 RU
<1.5 RU, 20,000 – 40,000 RU
• Baseline drift: <1 RU/min (15 – 40ºC)
• Experiment temperature range: 15 – 40ºC
• Temperature accuracy: +/- 0.2ºC
• Temperature difference: No more than +/- 15ºC from ambient
temperature
• CCD: 12-bit digital camera
• Data points measuring rate: 1 Hz for display, 50 frames/sec for
acquisition, average of 3 = display
Detection Limits
Note: The specifications given are typical values achieved. Many
experimental factors can expand or contract these working
ranges and limits.
• Concentration detection limit: sub – nM to mM levels (dependent on
ligand density, affinity constants, and MW of both ligand and
analyte)
• Molecular Weight (MW) detection limit: 201 daltons