ProteOn XPR36, User Manual

Page: 173 / 288

Maintenance Wizard Instructions
155
Postexperiment
This wizard provides routine detergent and acidic cleaning of the needles and
sample lines. After cleaning, the whole fluidics system is flushed with DDW.
This process is recommended whenever switching sensor chips is necessary.
It is an injection-based protocol run with cleaning solutions. This wizard
requires approximately 46 min to run. It requires an MNT chip.
To run the postexperiment cleaning wizard:
1. Set the running buffer to DDW.
2. On the Instrument Maintenance screen, click Post-Experiment.
3. Load solutions at the designated locations:
• If using sample racks:
2% Contrad-70, 520
μ
L at position L1 – L6
20 mM HCl, 520
μ
L at position K1 – K6
DDW, 520
μ
L at position J1 – J6
• If using microplates:
2% Contrad-70, 520
μ
L at position H7 – H12
20 mM HCl, 520
μ
L at position G7 – G12
DDW, 520
μ
L at position F7 – F12
4. Place the buffer inlet lines in distilled or deionized water and click Next.
5. Follow the prompts in the wizard.
6. The system remains in DDW at 25 μl/min when the maintenance
procedure is finished.
Clean MCM
The multichannel module (MCM) must be cleaned as often as necessary, but
at least four times a year. This procedure requires approximately 50 min.
To clean the multichannel module:
1. Obtain a CLN chip and let it equilibrate to ambient temperature.
2. Load distilled or deionized water as the running buffer and place both
inlet lines in the DDW.