ProteOn XPR36, User Manual

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Processing Data
111
• Selected — removes all data in selected regions and draws a
black line from the start and end points. The operation is applied
only to sensorgrams that are selected and only in the selected
graph windows.
To remove artifacts:
1. Select Artifact Removal on the Process menu and choose Auto.
2. Right-click and drag to define the sensorgram region containing the
artifact and choose Selected on the Process menu.
M
OVING A
S
ENSORGRAM
To align sensorgrams manually within the data windows:
1. Select interaction data to process.
2. Choose Manual Alignment on the Process menu.
3. Click the sensorgram in a data window and align the sensorgram.
To move all selected sensorgrams in a data window:
• Choose Manual Alignment, click in an empty area in the data
window, and drag all sensorgrams.
P
ERFORMING
R
EFERENCE
S
UBTRACTION
The two main types of reference subtraction are channel referencing and
double referencing.
Channel Referencing — Perform channel referencing using data collected on
a channel that does not have ligand immobilized or an irrelevant protein
immobilized or by using interspot data (chip areas between the spots, where
interaction data without immobilized ligand are collected).
Select Channel Reference on the Process menu and choose the type of
channel reference to use:
• Interspot and Column — most commonly used channel
references
• Row — used only if the ligand has been applied along channels
A1–A6, instead of the more commonly used L1–L6
• Correct for Excluded Volume — applies an excluded volume
correction to the selected channel reference. When selected, if an
excluded volume calibration has not been calculated, a wizard