ProteOn XPR36, User Manual

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Running Experiments
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To select an option for viewing the data while the experiment runs:
• To group the data by their associated ligand (vertical) channel, on
the Data screen click Group by Ligand
• To group the data by their associated analyte (horizontal) channel,
click Group by Analyte.
• By default, the data shown are baseline aligned (set to zero) at the
sample injection start time. To cancel this automatic alignment at
injection start, clear the Show Baseline Aligned Data option on the
View menu
To stop an experiment in the middle of a step:
• Click Abort. Aborting a step causes the experiment to stop as soon
as possible and saves any data collected in the step
To resume an aborted experiment:
• Click Start to resume an aborted experiment. The protocol begins
at the step after the aborted step
To start a new experiment on a used chip:
1. Select a new protocol to run using the Selected Protocol/Experiment
pull-down list in the Run tab.
2. In the Run tab, click Start. A message informs you that the previous
experiment run on this chip will be sealed and asks you if you want to
continue. A sealed experiment cannot be run again, even if it contains
steps that have not been run. Sealing an experiment helps ensure that it
contains a complete record of what happens to the chip over the entire
course of the experiment. When the experiment is continued, the
instrument status changes from Ready to Running.
Nonspecific Binding
Nonspecific binding, or NSB, is defined as the binding of an analyte or other
component(s) in the sample or running buffer directly to the sensor chip
surface and not to the target ligand. NSB is characterized by significant
binding response signals on the reference spots. These events have the
potential to skew assay results.
If NSB events occur, see Chapter 7 for a thorough discussion of causes and
prevention.