Promega Corporation ·
2800 Woods Hollow Road
·
Madison, WI 53711-5399 USA
·
Toll Free in USA 800-356-9526
·
Phone 608-274-4330
·
Fax 608-277-2516
· www.promega.com
Printed in USA.
Part# TM338
Revised 1/11
Page 9
3.
Instrument Calibration and Operation
An instrument workflow chart is provided in Section 8.
3.A. Instrument Power Up and Shut Down
To turn on the QuantiFluor™ Fluorometer, press the
ON/OFF
button. The instrument takes five seconds
to warm up. After the warm-up, the QuantiFluor™ Fluorometer is ready for use. Pressing the
ON/OFF
button again will turn off the unit.
QuantiFluor™-P units only:
If left idle for three minutes, the unit will automatically turn off to save
battery power.
A set of new batteries can last over 1,000 sample readings. If the batteries have low power or are not
positioned properly, the following warning message will appear: "Batt Level < 20% !! Caution !!"
QuantiFluor™-ST units only:
After switching off the unit, unplug the AC Adapter from the wall outlet.
3.B. LCD
The QuantiFluor™ Fluorometer has a backlit, adjustable-brightness LCD (Liquid Crystal Display) for
easier viewing. The contrast may change with temperature and may get lighter as the unit gets colder.
While on the HOME screen, adjust the brightness of the LCD using the
UP ARROW
and
DOWN
ARROW
buttons.
3.C. Detection Channel Configuration
The QuantiFluor™ Fluorometers have two fluorescence detection channels. Identify the configuration by
looking at the label on the back side of the QuantiFluor™ Fluorometer. See the Optical Specifications
table in Section 1.D to confirm the correct configuration for your application.
Select the appropriate channel by pressing the
A/B
button to toggle between the two channels. The display
will show a label in the lower left corner of the HOME screen to identify which channel is activated.
QuantiFluor™-ST Fluorometer (Cat.# E6090)
Channel A
UV (365nm
Ex
, 440–470nm
Em
),
Channel B
Blue (460nm
Ex
, 515–575nm
Em
)
QuantiFluor™-P Fluorometer (UV, Blue; Cat.# E6105)
Channel A
UV (365 nm
Ex
, 440–470nm
Em
),
Channel B
Blue (460 nm
Ex
, 515–575nm
Em
)
QuantiFluor™-P Fluorometer (Green, Blue; Cat.# E6100)
Channel A
Green (525nm
Ex
, >570nm
Em
)
Channel B
Blue (460nm
Ex
, 515–575nm
Em
)
