Powering the Luminometer
1. Remove the plastic packing material that holds the optical head in place during shipping.
2. Connect the power supply to the GloMax™ 96 Microplate Luminometer.
3. Connect the 9-pin serial cable to the luminometer and the computer.
4. Turn “ON” both the luminometer and your computer.
5. Install the GloMax™ Software provided on the CD-ROM that came with the luminometer.
Starting the Software
6. Double-click on the GloMax™ icon on your computer desktop to start the software.
7. Select “Run Promega Protocol” if you are running a Promega assay. A list of Promega protocols will appear. Select the
one that is appropriate for your assay. Alternatively, select “Create New Protocol” if you wish to write a new protocol, or
select “Open Saved Protocol” if you wish open an existing protocol that you saved previously.
Setting the Options on a New Protocol
8. When you choose “Create New Protocol”, a wizard tool will guide you through the setup.
9. First, select the number of injectors needed: 0, 1 or 2.
10. Use the arrow keys to change each setting from the default vaule. Click the “Accept” button to accept one setting and
proceed to the next parameter. To readjust a setting, click “Reset values for these parameters.”
For
noninjection protocols, you may choose to set a delay time before the luminometer begins reading the microplate.
You may also adjust the integration time per well, the number of repeated runs and the delay between repeated runs.
For
injection protocols, you may choose to measure your samples before the injection. You may also set the injection
volume for each injector and the delay between injection and measurement. For all measurements, you may adjust the
integration time per well.
11. Select the wells you wish to read. You can select or deselect the entire plate by clicking “All”. You can select or deselect
individual rows and columns by clicking on the letter or number identifying the row or column. Click on an individual well
to select or deselect that well.
12. Click “Finish” or “Save Protocol as” to store the protocol for future use.
13. To review or change settings, select “Options” from the Main Dialog Box.
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Printed in USA. Revised 3/09
Part# 9FB082
© 2006, 2009 Promega Corporation. All Rights Reserved.
ORDERING / TECHNICAL INFORMATION:
www.promega.com • Phone 608-274-4330 or 800-356-9526 • Fax 608-277-2601
Quick Start Guide
GloMax™ 96 Microplate Luminometer
INSTRUCTIONS FOR USE OF PRODUCTS E6501, E6511 AND E6521
P R O T O C O L
Quick
