Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
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www.promega.com
TM276 · Revised 5/21
5.D. Running a Promega Protocol
The GloMax
®
20/20 Luminometer accommodates the measurement requirements for luminescent assays from
Promega. Touch
Run Promega Protocol
from the Protocol Menu (Figure 4), and select the protocol that corresponds
to your assay system. For example, if you are using the Steady-Glo
®
Assay for a luciferase gene reporter study, select
Steady-Glo
. The luminometer will open a Steady-Glo
®
protocol with the recommended measurement settings
automatically programmed. Touch
OK
to accept the parameters and return to the Home Screen. You are now ready to
measure your samples.
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Figure 4. The Protocol Menu.
5.E. Creating a New Protocol
If you touch
Create New Protocol
, the GloMax
®
20/20 Luminometer prompts you to adjust the integration time
(Figure 5). The integration time is another term for the measurement duration. Touch
Integration
, then use the up
and down arrow keys to increase or decrease the integration time. When you are finished, touch
Save As
to save your
protocol under a specific name of your choice. Alternatively, touch
OK
to return to the Home Screen to measure your
samples. The luminometer will automatically name your protocol “New Protocol” and return to the Home Screen.
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Figure 5. A typical parameters screen.
