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ProEM-HS

Camera System

4411-0149

Issue 

2

July 6, 2015

Summary of Contents for ProEM-HS

Page 1: ...ProEM HS Camera System 4411 0149 Issue 2 July 6 2015...

Page 2: ...C Windows and Windows Vista are registered trademarks of Microsoft Corporation in the United States and or other countries The information in this publication is believed to be accurate as of the publ...

Page 3: ...2 1 4 CCD Arrays 19 2 1 5 Cooling 20 2 1 5 1 Internal Fan 20 2 1 5 2 External Cooling Circulator 20 2 1 5 3 Coolant Ports 20 2 1 6 Rear Panel Connectors 21 2 2 Cables 22 2 3 Certificate of Performance...

Page 4: ...p and Configuration 47 5 1 2 Data Acquisition 49 5 2 Spectroscopy 51 5 2 1 System Set Up and Configuration 52 5 2 2 Rotational Alignment and Focus 54 5 2 2 1 Acton Series Spectrograph 55 5 2 2 2 IsoPl...

Page 5: ...ential Mode for Frame Transfer EMCCD 98 7 10 6 3 Full Frame Readout for Full Frame EMCCD 99 7 10 7 Readout Time 100 Chapter 8 Experiment Synchronization 101 8 1 Shutter Control Mode 101 8 2 Timing Mod...

Page 6: ...adual Deterioration of Cooling Capability 149 13 8 Data Overrun Due to Hardware Conflict Message 149 13 9 Device is Not Found 150 13 10Device is Occupied 150 13 11Ethernet Network is Not Accessible 15...

Page 7: ...nty 181 Sealed Chamber Integrity Limited 12 Month Warranty 182 Vacuum Integrity Limited 12 Month Warranty 182 Image Intensifier Detector Limited One Year Warranty 182 X Ray Detector Limited One Year W...

Page 8: ...6 8 Typical Define Spectrograph Dialog 73 Figure 6 9 Typical Install Remove Spectrograph Dialog 73 Figure 7 1 Clean Cycles Timing Diagram 87 Figure 7 2 EMCCD Array Structure 89 Figure 7 3 Binning and...

Page 9: ...al Hardware Setup Dialog Custom Chip Tab Skip Serial Register Clean 142 Figure 12 8 Typical Readout Time Dialog 142 Figure 13 1 Acquisition Display and Invalid ROI 144 Figure 13 2 Acquisition Display...

Page 10: ...e 9 1 LightField Kinetics Parameter Configuration 116 Table 9 2 WinX 32 Hardware Parameter Configuration for Kinetics 117 Table 9 3 WinX 32 Experiment Setup Configuration for Kinetics 117 Table 9 4 Co...

Page 11: ...m This document provides all information necessary to safely install configure and operate the ProEM HS beginning with the system s initial installation 1 2 Related Documentation Table 1 1 provides a...

Page 12: ...nstruments LightField 64 bit data acquisition software Chapter 6 WinX 32 First Light Provides a step by step procedure for placing a ProEM HS camera system in operation for the first time when using P...

Page 13: ...ontact information is also provided 1 4 Conventions The following conventions are used throughout this manual WinX 32 is a generic term that is used to indicate one or more of the following data acqui...

Page 14: ...ering with its operation is also prohibited Inspect the supplied power cord If it is not compatible with the power socket replace the cord with one that has suitable connectors on both ends WARNING Re...

Page 15: ...0 seconds before switching it on the TEC Fault LED might come on if the power supply on off state is switched too quickly Use caution when triggering high current switching devices near the system e g...

Page 16: ...16 ProEM HS User Manual Issue 2 This page is intentionally blank...

Page 17: ...Typical ProEM HS System Components Standard items include ProEM HS Camera and Users Manual Power Supply and Cable Ethernet Card Gigabit Ethernet cable MCX to BNC Cable s Certificate of Performance Da...

Page 18: ...tween an Electron Multiplying CCD EMCCD and a traditional CCD is an extended serial register in the EMCCD device See Figure 2 2 Figure 2 2 ProEM HS Frame Transfer versus Kinetics EMCCD Array Structure...

Page 19: ...the data acquisition software For instance if an experiment requires rapid image acquisition then the CCD s on chip binning can be set to increase frame rates 2 1 3 Power All voltages required by Pro...

Page 20: ...ray and Cools the electronics An internal Peltier device directly cools the cold finger on which the CCD is mounted Air drawn into the camera through the back of the camera removes the heat produced b...

Page 21: ...level input with a 10 k pull up resistor Allows data acquisition and readout to be synchronized with external events Positive or negative default edge triggering can be selected For information about...

Page 22: ...621 Cat 5e 6 Ethernet cable Connects the ProEM HS camera to the host computer The detector and the computer may be more than 50 meters apart Contact the factory to order longer cables 5 m 16 4 ft MCX...

Page 23: ...phs with easy to use tools for experimental setup data acquisition and post processing LightField makes data integrity priority 1 via automatic saving to disk time stamping and retention of both raw a...

Page 24: ...Manual describes how to install and use the application program A PDF version of the manual is provided on the installation CD Additional information is available in the program s online help PVCAM A...

Page 25: ...stallation Additional space is required for data storage the amount of space required depends on the number and size of images spectra collected Disk level compression programs are not recommended Dri...

Page 26: ...stem These hoses mate with the ProEM HS fittings part number 2550 0630 Additional hoses can be ordered separately from Princeton Instruments CAUTION To prevent voiding the ProEM HS warranty only use t...

Page 27: ...er to for information about available spectroscopy mounts for the ProEM HS camera system Table 2 4 Available Spectroscopy Mounts for the ProEM HS Part Number Description 7050 0083 C to Spectroscopy Mo...

Page 28: ...d a need to clean the optical window arise due to the accumulation of atmospheric dust we advise that the drag wipe technique be used Before starting the procedure run the camera and disable the shutt...

Page 29: ...eady installed in the host computer install it Section 4 8 Application Software Installation on page 40 and relevant software manuals 6 Depending on application attach lens to the camera or mount the...

Page 30: ...2 3 Data Acquisition on page 59 WinX Imaging Section 6 2 3 Focus the System on page 67 Spectroscopy Section 6 3 6 Focus the System on page 78 14 Adjust the focus for the best image or spectral lines L...

Page 31: ...py Experiment Layout with Air cooled Camera and IsoPlane 4411 0149_0005 POWER SUPPLY ETHERNET CABLE POWER CABLE GIGE GIG E CARD SPECTROMETER 100 240 V SAMPLE CAMERA HOST COMPUTER 4411 0149_0006 POWER...

Page 32: ...32 ProEM HS User Manual Issue 2 Figure 3 4 Typical Imaging Experiment Layout with Air Liquid cooled Camera Figure 3 5 Typical Spectroscopy Experiment Layout with Air Liquid cooled Camera...

Page 33: ...ormation refer to Contact Information on page 184 Hardware installation consists of Installing a dedicated GigE interface card Attaching a lens to a C mount on the camera or to a C to F mount adapter...

Page 34: ...Cables MCX to BNC adapter cables Hoses Two coolant hoses with ProEM HS compatible quick disconnects ProEM HS System User Manual Application Software WinView 32 or WinSpec32 Ver 2 6 10 or later CD ROM...

Page 35: ...to ambient light will over saturate it This may increase dark charge significantly If the camera remains saturated after all light sources are removed you may have to bring the camera back to room tem...

Page 36: ...reads are flush with the back surface of the adapter Note the depth at the front surface remove the lens and then re insert the adapter into the camera nose 4 4 Mounting the Adjustable C to Spectrosco...

Page 37: ...ght baffle mount the light baffle to the front of the adapter 4 Gently rotate the camera and sliding tube assembly as you insert it into the spectrograph s exit port 5 With the spectrograph camera and...

Page 38: ...adjustment tighten the recessed locking setscrews in the adapter flange to lock the position 4 5 Positioning ProEM HS Masks NOTE This option is not available for ProEM HS 1024B cameras The ProEM HS c...

Page 39: ...the camera is at table height and the circulator is on the floor 3 Make the coolant connections between the circulator and the camera It does not matter which hose from the circulator is plugged into...

Page 40: ...problems continue to Step 9 9 Turn the camera on 10 Launch the application software 4 8 Application Software Installation This section provides detailed procedures for the installation of supported a...

Page 41: ...and follow the on screen prompts Figure 4 5 illustrates a typical InstallShield Wizard dialog Figure 4 5 Typical LightField InstallShield Wizard Dialog 2 After the installation has been completed reb...

Page 42: ...ion Type dialog is displayed select the desired type of installation Typical installs all required drivers and the most commonly installed program files Complete installs all available application dri...

Page 43: ...ilable device s and load the appropriate icons into the Available Devices area in the Experiment workspace 4 When an icon is dragged into the Experiment Devices area the appropriate expanders will be...

Page 44: ...hen the Welcome dialog is displayed the check box should remain unchecked Click Next See Figure 4 8 Figure 4 8 Typical WinX Camera Detection Wizard Dialog 4 Follow the on screen prompts to perform the...

Page 45: ...ghtField Users Manual Refer to Table 1 1 for document numbers information During data acquisition the CCD array is exposed to a source and charge accumulates in the pixels After the defined exposure t...

Page 46: ...is selected cannot be stored Preview mode is particularly convenient for familiarization and setting up For ease in focusing the screen refresh rate should be as rapid as possible achieved by operatin...

Page 47: ...case review the software manual or have it available while performing this procedure The camera has a frame transfer array and a software controlled internal shutter The system is being operated in i...

Page 48: ...Status bar across the bottom of the window includes icons for temperature status and orientation Temperature Status reports the current system temperature and whether the set temperature has been rea...

Page 49: ...rocedure to acquire live data 1 Click on the View tab located above Experiment Devices to change focus to the View area See Figure 5 4 Figure 5 4 View Area 2 Click Run to initiate Preview mode In this...

Page 50: ...A D converter is at full scale NOTE A 16 bit A D is at full scale when the brightest parts of an image reach an intensity of 65535 Adjust the aperture so that it is slightly smaller i e a higher f st...

Page 51: ...nual shutter The system is being operated in spectroscopy mode The spectrograph has an entrance slit shutter that is controlled by the ProEM HS via the Shutter connector An underlying assumption of th...

Page 52: ...nd the ProEM HS Shutter connector Verify the shutter knob for the ProEM HS s manual shutter is pushed in i e manual shutter is open External Slit Shutter A shutter assembly mounted externally to the s...

Page 53: ...sired temperature can be configured Orientation is displayed because the default readout port is Electron Multiplied Clicking on the icon opens a panel that describes how the image orientation has bee...

Page 54: ...ata display If the problem is fixed stop acquisition or proceed to Section 5 2 2 Rotational Alignment and Focus on page 54 If you do not hear a shutter operating and you have performed step a through...

Page 55: ...or the alignment If this is the case use a wavelength setting of 0 0 nm for alignment purposes Figure 5 8 View Area 3 Open the Spectrometer expander select the grating and set the center wavelength to...

Page 56: ...ment Before Rotational Alignment 8 Adjust the rotational alignment You do this by rotating the camera while watching a live display of the line Click on the peak you want to monitor during the rotatio...

Page 57: ...on is active for the center graph to allow you to monitor the FWHM information to achieve the narrowest line width The way focusing is accomplished depends on the spectrograph as follows Long focal le...

Page 58: ...ect the Align Spectrometer function from the Experiment menu to open the Spectrometer Alignment dialog This dialog describes the changes that LightField will make to the current setup to assist you in...

Page 59: ...ync Lowering the temperature 3 Begin running Acquire mode Data will be acquired and displayed stored based on the experiment settings 4 Once data acquisition is complete either Leave the camera power...

Page 60: ...60 ProEM HS User Manual Issue 2 This page is intentionally blank...

Page 61: ...the software manual Refer to Table 1 1 for document number information During data acquisition the CCD array is exposed to a source and charge accumulates in the pixels After the defined exposure tim...

Page 62: ...ta collected can be automatically stored so the completed dataset may include multiple frames with one or more set of accumulations This mode is typically selected during actual data collection One li...

Page 63: ...rame transfer array and a software controlled internal shutter The ProEM HS system is operating in imaging mode The target image being used is either a clear image text or a drawing that will verify t...

Page 64: ...tab See Figure 6 2 Figure 6 2 Typical WinView 32 Hardware Setup Dialog Controller Camera Tab Verify and or configure the parameters indicated in Table 6 1 Table 6 1 WinView 32 Hardware Setup Configur...

Page 65: ...to be briefly lost and then quickly re established When reading the actual temperature reported by the application software there may be a small difference between the configured and reported temperat...

Page 66: ...arameter Value Notes Main Tab Exposure Time 50 ms Accumulations Number of Images 1 ROI Tab These parameters define the region of interest ROI Imaging Mode When using WinSpec Imaging Mode must be selec...

Page 67: ...ightest regions of the image to determine if the A D converter is at full scale NOTE A 16 bit A D is at full scale when the brightest parts of the image reach an intensity of 65535 Adjust the aperture...

Page 68: ...ource e g Princeton Instruments IntelliCal has been mounted in front of the entrance slit of the spectrograph Any light source with line output can be used Standard fluorescent overhead lamps provide...

Page 69: ...he manufacturer s user manual for information about operating the shutter 5 Turn on the ProEM HS s power supply NOTE The camera must be turned on before WinSpec 32 is launched 6 Turn on the host compu...

Page 70: ...etup Dialog Controller Camera Tab Verify and or configure the parameters as indicated in Table 6 4 Table 6 4 WinSpec 32 Hardware Configuration Parameter Value Notes Controller Type This information is...

Page 71: ...ture will remain stable to within 0 05 C NOTES 1 Some overshoot may occur which may cause temperature lock to be lost briefly and then quickly re established When reading the actual temperature report...

Page 72: ...iment Setup Configuration Parameter Value Notes Main Tab Exposure Time 50 ms Accumulations Number of Images 1 ROI Tab These parameters define the region of interest ROI Spectroscopy Mode Full Loads th...

Page 73: ...iate spectrograph configuration parameters 1 From the WinSpec 32 menu bar select Spectrograph Define The Define Spectrograph dialog is displayed See Figure 6 8 Figure 6 8 Typical Define Spectrograph D...

Page 74: ...either a spectral band image or a graph will be displayed 2 Background noise will decrease as the camera cools to its default temperature 2 Turn off the light source at the spectrograph s entrance sli...

Page 75: ...noise pattern or low intensity graph Yes Proceed to Section 6 3 5 Rotational Alignment on page 75 No Stop data acquisition Contact Customer Support for assistance Refer to Contact Information on page...

Page 76: ...the Exposure Time to maintain optimum near full scale signal intensity 4 Wait until the camera temperature locks at its default temperature 5 Verify that the spectroscopy mount adapter moves freely at...

Page 77: ...output can be used Standard fluorescent overhead lamps have good calibration lines as well If there are no line sources available a broadband source such as tungsten can be used If this is the case u...

Page 78: ...points are X 0 and Y 40 Save as Default check box is checked Click OK to display the three stacked graphs Align the large cursor with a prominent peak and rotate the detector until the peak aligns wit...

Page 79: ...on Series spectrograph 1 From the WinSpec 32 menu bar select Process Focus Helper to activate the Focus Helper function The Focus Helper can be used to determine the narrowest line width by automatica...

Page 80: ...ition Acquire to begin data acquisition Data are then acquired displayed and stored based on specific configuration settings 4 Once data acquisition is complete available options include Leave the cam...

Page 81: ...nal before the start of the exposure time At the end of the exposure time the CCD is readout and cleaning starts again The effective exposure time of an array depends on the active readout mode that h...

Page 82: ...high speed CCD with rather high read noise As the result of EM gain calibration values 1 to 1000 are mapped linearly to the internal serial clock voltages that vary the multiplication gain for a one t...

Page 83: ...EM HS camera connected to the host computer 2 If running close the data acquisition program i e WinView 32 WinSpec 32 or LightField 3 If the ProEM HS camera uses a manual shutter close the shutter If...

Page 84: ...is strongly recommended to all the system to achieve optimum thermal stability When vibration may affect results the internal fan can be turned off as long as it has been verified that coolant is cir...

Page 85: ...emperature speed and EM gain To counter this ProEM HS has a built in bias active stabilization engine or BASE The camera reads overscan pixels i e pixels outside the region of the CCD to account for a...

Page 86: ...ted longer exposure times and therefore longer signal accumulation in the CCD will improve the S N ratio approximately linearly with the length of exposure time There is however a maximum time limit f...

Page 87: ...t applications Even so it is a good idea to know what these entries mean with regard to cleaning Table 7 2 provides information about the parameters used to configure Clean Cycles Table 7 2 Clean Cycl...

Page 88: ...is tied to an external trigger In WinX 32 this cleaning is active when External Sync timing mode is selected In LightField this cleaning is active when Trigger Response is set to Readout Per Trigger...

Page 89: ...r Regions of Interest ROI and or binning can be used to improve the time resolution limited to a millisecond regime For more information about the CCD exposure readout operations refer to Section 7 10...

Page 90: ...tc Only 25x 100x EM gain is required to overcome the read noise of the camera Using excessive EM gain will not improve the overall signal to noise ratio SNR NOTE An image read out of the Multiplicatio...

Page 91: ...llows the digitization of larger signals 2 Medium This setting is suitable for experiments within the mid level intensity range 3 High This setting is suitable for applications where low level signals...

Page 92: ...10 4 Regions of Interest ROI A Region of Interest ROI may be defined as an entire CCD array or it may be a rectangular subregion of the array An ROI is defined in the X and Y direction by A start pixe...

Page 93: ...aved The information for the active data display can be reviewed using the File Information function accessed from either the File or Display Context menu CAUTION When configuring a partial frame ROI...

Page 94: ...not apply to LightField 7 10 5 1 Array Orientation ProEM HS cameras use square CCDs Therefore binning can be performed along either direction of the CCD Binning along columns parallel mode provides in...

Page 95: ...extremely useful in applications requiring continuous imaging i e 100 duty cycle Once a frame has been exposed and transferred into the frame transfer area the next exposure starts immediately and co...

Page 96: ...3 frames 0 100 seconds NOTE Because the first frame is exposed for 10 ms and the others for 30 ms the first frame may appear dimmer when compared with the other frames that have been acquired In Frame...

Page 97: ...alculated using the formula TN texp N tR Where TN Total time taken to capture a sequence of N frames texp exposure time N total number of frames in a sequence tR readout time for one frame From the ti...

Page 98: ...me rate In WinX 32 Full Frame mode is selected from Hardware Setup Controller Camera tab In LightField Full Frame mode is selected on the Sensor expander Example Full Frame Mode for Frame Transfer EMC...

Page 99: ...l letters represent different amounts of charge including both signal and dark charge This section explains readout at full resolution where every pixel is digitized separately In this drawing the cha...

Page 100: ...into this node it is digitized Only after all pixels in the first row are digitized is the second row moved into the shift register The order of shifting in our example is therefore A1 B1 C1 D1 A2 B2...

Page 101: ...shutter is being controlled by ProEM HS shutter operation can be coordinated with the experiment NOTE Shutter Control mode combined with Timing Mode provides a wide variety of precision experiment syn...

Page 102: ...dance High impedance Edge Trigger Trigger Determined By Rising Edge or Falling Edge is specified on the Experiment Setup Timing tab Trigger expander Supported Timing Trigger Response modes for Frame T...

Page 103: ...ed into the software and is then read out If a trigger arrives during the exposure readout of the previous frame it is ignored NOTE For a sequence of one frame bulb mode and trigger first mode are the...

Page 104: ...ntinuous cleans is enabled the camera will check for an active edge at the EXT SYNC connector before entering a continuous clean cycle If none has occurred a cycle will begin and complete The EXT SYNC...

Page 105: ...ed to fall on the CCD array Figure 8 4 illustrates a flowchart that highlights the differences between these two modes NOTES 1 When using LightField Fast mode is always used 2 When using WinX 32 the c...

Page 106: ...tion frames are displayed as time permits Frames complete Stop acquisition command sent from computer to camera Stop Stop No Yes No Yes No No Yes Yes Cleans performed Cleans performed Computer program...

Page 107: ...netics Mode on page 109 for more information about this type of image acquisition and readout 8 4 LOGIC OUT Control The TTL compatible output from the LOGIC OUT connector on the rear panel can be used...

Page 108: ...ire and is waiting for an external trigger through the EXT SYNC connector before exposing the CCD The level goes low when a trigger is detected exposure begins The Wait for Trigger WFT signal goes hig...

Page 109: ...ond time resolution However careful attention must be paid to the following optical and timing considerations The rows furthest from the serial register need to be illuminated The rest of the active a...

Page 110: ...plication gain EM Gain in ProEM HS further improves the SNR when the signal is below the read noise In kinetics mode a portion of the CCD image is optically masked in order to minimize the cross talk...

Page 111: ...lution between subframes can be achieved 4411 0149_0033 MULTIPLICATION GAIN LOW NOISE FOR HIGH SPEED FOR STANDARD FRAME TRANSFER AREA SENSOR AREA ILLUMINATION WINDOW HEIGHT 60 ROWS ROWS FARTHEST FROM...

Page 112: ...e as configured in software IMAGE SHIFT is high during Image to Storage time READ OUT is high during Vertical Transfer Digitization SHUTTER is high during Shutter open comp All Exposures Shift Cycles...

Page 113: ...ing of the first exposure shift kinetics cycle and closes at the end of the last exposure shift cycle Disabled simply means that the shutter will not operate during the experiment Disabled closed is u...

Page 114: ...ter and Trigger Expanders Figure 9 8 Kinetics Operation Example Single Trigger Readout Per Trigger SELECT THE TRIGGER MODE SINGLE OR MULTIPLE 4411 0149_0037 4411 0149_0036 4411 0149_0038 TRIGGER IN EX...

Page 115: ...n provides the information necessary to configure a kinetics experiment Figure 9 10 illustrates the hardware setup for a typical Kinetics experiment Figure 9 10 Typical Kinetics Experiment Hardware Co...

Page 116: ...of exposed rows Temperature Setpoint The default temperature setting is read from the camera When the array temperature reaches the set temperature that status will be reported as Locked in the Senso...

Page 117: ...ition Experiment Setup Verify and or configure the parameters as indicated in Table 9 3 Table 9 2 WinX 32 Hardware Parameter Configuration for Kinetics Parameter Value Notes Controller Type This infor...

Page 118: ...illuminated rows all while delivering the same temporal resolution as standard kinetics For the fastest possible acquisition this mode requires that an external mask blocks light from all but a windo...

Page 119: ...lluminated As can be seen in Figure 9 11 these rows are immediately below the frame transfer area The rest of the active area must be optically masked Single trigger Readout Per Trigger mode allows th...

Page 120: ...120 ProEM HS User Manual Issue 2 This page is intentionally blank...

Page 121: ...to be specified within the data acquisition software Unlike defining a smaller ROI which also involves reading out fewer pixels Custom Chip Custom Sensor mode does not incur overhead from discarding o...

Page 122: ...ubregion is to be acquired and read out ROI Method Reading out the 128 x 128 pixel subregion requires 8 2 ms which corresponds to a frame rate of 122 fps i e 1 0 0082 Custom Chip Custom Sensor The rea...

Page 123: ...area In a multi frame acquisition unwanted signal photoelectrons and dark charge will contribute increasingly to each successive frame until the entire sensor has been read out once at which point a...

Page 124: ...e factory for guidance before customizing the chip definition In WinX 32 if Custom Chip has been installed selecting Show Custom Chip check box on the Controller Camera tab adds the Custom Chip tab to...

Page 125: ...only the reduced active area is exposed This will prevent unwanted charge from spilling into the active area or being transferred to the shift register By default if there are no Pre Dummy rows the se...

Page 126: ...or button on the Sensor expander In the Custom Timing panel you can select from among the listed vertical shift rate choices as illustrated in Figure 10 6 Vertical shift rate is the time required to s...

Page 127: ...Chapter 10 Custom Chip Mode 127 In WinX 32 the equivalent function is located on the Hardware Setup Camera Controller tab See Figure 10 7 Figure 10 7 WinX 32 Vertical Shift 4411 0149_0048...

Page 128: ...128 ProEM HS User Manual Issue 2 This page is intentionally blank...

Page 129: ...ta that must be considered Increasing the frames per second tends to decrease data quality NOTE The information within this chapter is for ProEM HS 512BX3 camera systems 11 1 Imaging Applications Perf...

Page 130: ...al Issue 2 3 From the Experiment Type pull down menu select Imaging to display the set of associated configuration parameters See Figure 11 2 Figure 11 2 High Speed Camera Configuration Imaging Applic...

Page 131: ...gs NOTE Click Restore to Defaults to restore experiment settings to default ProEM HS values 7 Return to the Experiment Settings tab and on the Common Acquisition Settings expander enter the number of...

Page 132: ...sue 2 3 From the Experiment Type pull down menu select Spectroscopy to display the set of associated configuration parameters See Figure 11 4 Figure 11 4 High Speed Camera Configuration Spectroscopy A...

Page 133: ...e entire sensor has been read out once at which point a steady state will be achieved For example suppose the 20 rows at the top i e nearest to the readout register of a 100 row sensor have been defin...

Page 134: ...with the high speed settings NOTE Click Restore to Defaults to restore experiment settings to default ProEM HS values 7 Return to the Experiment Settings tab and on the Common Acquisition Settings ex...

Page 135: ...EM HS uses a highly advanced single window vacuum design This means the vacuum window is the only optical surface encountered by incident photons before they reach the EMCCD detection surface Although...

Page 136: ...two modes provides increased spectral rates over those attained by simply configuring a custom Region of Interest NOTE Princeton Instruments PVCAM PICam and National Instruments LabVIEW users can also...

Page 137: ...llowing procedure to reduce spectral readout time when using LightField 1 Examine the Readout Time Readout expander and the Frames Per Second upper right hand corner of the Experiment workspace Based...

Page 138: ...and Closing Delay to 0 ms Readout Time 1 648 ms FPS 603 8 Set Vertical Shift Rate to 2 s Readout Time 816 s FPS 1210 9 In the Custom Sensor pane set Active Area Height to 208 rows and change the Top a...

Page 139: ...12 Tips 139 Figure 12 2 illustrates relevant settings and results Readout Time and FPS at the conclusion of this procedure NOTE Note that the Exposure Time not shown was 0 ms Figure 12 2 LightField S...

Page 140: ...uction 1 On the Experiment Setup ADC tab select Rate 4 MHz Readout Port Multiplication Gain 2 On the Hardware Setup Controller Camera tab select Vertical Shift 2 s 3 Select Easy Bin from the Acquisiti...

Page 141: ...ies to 0 See Figure 12 5 Figure 12 5 Typical Hardware Setup Dialog Custom Chip Tab 7 Now go to the Hardware Setup Controller Camera tab 8 Select Easy Bin from the Acquisition menu Select ALL This will...

Page 142: ...Figure 12 7 Figure 12 7 Typical Hardware Setup Dialog Custom Chip Tab Skip Serial Register Clean 12 Acquire a spectrum 13 Select Readout Time from the Acquisition menu The readout time is reported on...

Page 143: ...tion Started but Viewer Contents Do Not Update page 145 Baseline Signal Suddenly Changes page 145 Camera Not Found page 146 Camera Stops Working page 146 Camera1 or similar name in Camera Name field p...

Page 144: ...divisible by 4 Figure 13 1 shows an invalid ROI because the 30 in 30 x 512 x 1 is not evenly divisible by 4 If the number of horizontal pixels or superpixels if there is binning in the horizontal is n...

Page 145: ...13_49 raw is displayed If there is a filename click on the camera icon The file data will be cleared from the viewer and the live data will then be displayed Figure 13 2 Acquisition Display 13 3 Basel...

Page 146: ...located on the Hardware Setup dialog 5 If the camera is connected to the computer and has power the wizard should be able to locate it and load it into the Camera Detection Wizard Detected Hardware d...

Page 147: ...indows directory The new name will then be used by the system until the Camera Detection Wizard is run again Perform the following procedure to change the default Camera Name 1 Close the application p...

Page 148: ...to manufacturer s instructions for removing excess air and adding coolant Circulator pump is not working If you do not hear the pump running when the circulator is powered on turn off the circulator a...

Page 149: ...facility 13 8 Data Overrun Due to Hardware Conflict Message If this dialog appears when you try to acquire a test image acquire data or run in focus mode check the CCD array size and then check the DM...

Page 150: ...inX or LightField can detect a Princeton Instruments GigE camera but they may have been closed as part of your computer security such as an anti virus program or a firewall Contact your Information Te...

Page 151: ...ftware Official WinX32 GigE Open the default Pleora directory Typically it is located at C Program Files Common Files Pleora The EbDriverTool32 exe or EbDriverTool exe file may be in a subdirectory 2...

Page 152: ...BUS Driver Installation Tool Dialog 3 From the list of devices identify the appropriate Ethernet card Within the Action column select Install Manufacturer Driver from the appropriate pull down menu 4...

Page 153: ...oot WinView 3 Note the array size on the Setup Hardware Controller CCD tab or the Acquisition Experiment Setup Main tab Full Chip dimensions If your camera contains a large array such as a 2048x2048 a...

Page 154: ...r a cable comes loose while the application software is running in Focus mode 13 14 Smeared Images ProEM HS frame transfer CCDs allow simultaneous exposure readout operations Refer to Section 7 10 6 E...

Page 155: ...one of the ports To Modify the Default IP Address for a Port 1 Click on the Windows Start button and click on Control Panel Figure 13 13 Windows Start Button 2 On the Control Panel click on Device Ma...

Page 156: ...the IP Address 192 168 2 1 with 4 so the IP Address is now 192 168 4 1 Figure 13 16 Settings Tab 6 Click on OK 7 When the System Settings Change dialog appears click Yes to restart your computer now...

Page 157: ...cationsa Specification ProEM HS 512BX3 ProEM HS 1024BX3 CCD e2v CCD97B e2v CCD201B Image Type Monochrome Monochrome Resolution 512 x 512 1024 x 1024 Pixel Size 16 m x 16 m 13 m x 13 m Active Area 8 2...

Page 158: ...ry Unichrome coating of the CCD is available For broadband applications a choice of MgFl2 or broadband AR coating is available upon request A 4 Input Power Specifications All voltages required by ProE...

Page 159: ...re unrestricted flow from the enclosure to an open environment is required Internal Cooling Fan The internal cooling fan is rated at 24 CFM at full power A 6 Mounts Available mounts are C mount Standa...

Page 160: ...he coolant circulator s specifications regarding circulator compatible hose fittings If a Princeton Instruments CoolCUBEII circulator is ordered with the camera hoses are supplied with appropriate Coo...

Page 161: ...mensions are in inches mm unless otherwise noted The following outline drawings are provided in this appendix ProEM HS Camera ProEM HS Outline Drawing C Mount ProEM HS Outline Drawing Spec Mount ProEM...

Page 162: ...59 7 2 90 73 7 1 00 32 UN THREAD C MOUNT ADJUSTABLE 4 063 20 UN MALE FEMALE THREAD 0 0 51 12 8 1 26 31 9 1 49 37 8 0 31 7 9 0 0 63 15 9 0 62 15 7 1 72 43 8 1 76 44 6 MCX CONNECTORS STATUS LED 2 PIN LE...

Page 163: ...15 2 ADJUSTABLE C MOUNT 0 0 590 14 99 CCD LOCATION 3 284 7 777 1 109 0 1 12 28 6 1 12 28 6 0 83 21 1 COOLANT INLET OUTLET QUICK DISCONNECT FITTINGS 2X 10 32 UNF ALL SIDES 0 1 49 37 8 1 26 31 9 0 51 1...

Page 164: ...Manual Issue 2 B 2 ProEM HS Power Supply Figure B 3 ProEM HS Power Supply Outline Drawing 0 1 06 4 02 2 16 0 2 15 4 29 0 18 POWER CONNECTOR RED LED GREEN LED LINE CORD INPUT POWER ON OFF SWITCH 0 9 8...

Page 165: ...6 26 159 1 6 80 172 7 0 1 30 33 0 3 00 76 2 5 00 127 0 8 00 203 2 0 80 20 3 7 20 182 9 2 45 62 2 0 6 12 155 3 1 83 46 4 3 89 98 9 0 0 68 17 3 1 77 45 0 3 69 93 7 DO NOT COVER VENTILATION HOLES 0 2 00...

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Page 167: ...to Table C 1 for information about adapter kits and associated installation information for available spectrographs NOTE When mounted to a spectrograph the text on the back of the ProEM HS should be r...

Page 168: ...iding tube assembly on the camera flange on and rotate the assembly so the baffle aligns with the array You may need to open the manual shutter 7 Tighten the screws 8 Gently rotate the camera and slid...

Page 169: ...pplied with the camera in the groove on the camera flange 4 If there is a baffle in place align the camera accordingly Usually this means that the text on the back of the detector will be right side u...

Page 170: ...r If there is a spacer plate mounted to the sliding tube remove it 3 Place the flat side of the adapter plate against the face of the camera 4 Insert the threaded C mount adapter through the center ho...

Page 171: ...e sliding tube from the spectrometer first loosen the two setscrews that secure it and then rotate the tube as you pull it out If you have removed the sliding tube from the spectrometer rotate the sli...

Page 172: ...nd sliding tube assembly as you insert it into the spectrometer 5 Secure the sliding tube with the two spectrometer setscrews one coming down from the top and the other from the side NOTE Adapter part...

Page 173: ...rotate the camera and sliding tube assembly as you insert it into the spectrometer 5 Secure the sliding tube with the two spectrometer setscrews one coming down from the top and the other from the si...

Page 174: ...174 ProEM HS User Manual Issue 2 This page is intentionally blank...

Page 175: ...ed ADC Resolution Bit Depth Continuous Cleans Clean Until Trigger Controller Gain Analog Gain Custom Chip Custom Sensor Custom Timing Custom Timing Disabled Closed Shutter Always Closed Shutter Disabl...

Page 176: ...Area Right Margin Pre Dummy Rows Parallel to Shift Register Active Area Top Margin Pre Dummy Shift Register Columns Active Area Left Margin PreOpen Shutter Open Before Trigger Shutter Readout Port Qua...

Page 177: ...r Disabled Closed Shutter Always Open Shutter Disabled Open Shutter Analog Gain Controller Gain Bit Depth ADC Resolution Clean Cycle Height Number of Strips per Clean Clean Serial Register Skip Serial...

Page 178: ...igger DIF Sensor Readout Region expander functions Easy Bin Shift Per Trigger DIF Dual Trigger Mode DIF Shutter Mode Shutter Control Speed ADC Rate Temperature Setpoint Target Temperature Trigger Dete...

Page 179: ...179 Declaration of Conformity This section of the ProEM HS system manual contains the Declaration of Conformity for ProEM HS system A system includes the ProEM HS camera with a ProEM HS power supply...

Page 180: ...180 ProEM HS User Manual Version 2...

Page 181: ...evice which carries NO WARRANTIES EXPRESS OR IMPLIED this product against defects in materials or workmanship for a period of up to one 1 year after shipment During this period Princeton Instruments w...

Page 182: ...urn damage which carry NO WARRANTIES EXPRESSED OR IMPLIED all image intensifier products for a period of one 1 year after shipment See additional Limited One 1 year Warranty terms and conditions above...

Page 183: ...our option an authorized service center 4 Before products or parts can be returned for service you must contact the Princeton Instruments factory and receive a return authorization number RMA Products...

Page 184: ...ential damages so the above provisions may not apply to you 12 When contacting us for technical support or service assistance please refer to the Princeton Instruments factory of purchase contact your...

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