Photon Systems Instruments AquaPen-C AP 110-C, Instruction Manual

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5.1.2 M
EASUREMENT
No device calibration is required before making chlorophyll fluorescence measurement. Results of fluorescence measurement are
affected by the device settings and the physiology of the sample.
Steps for Chlorophyll Fluorescence measurements with AquaPen-C:
•
Fill the cuvette with the sample of algae or cyanobacteria and close the cuvette with the stopper. Fill the cuvette with 3 ml of the
sample. Minimal volume for accurate measurements is 2 ml.
•
Place the cuvette with the sample inside the AquaPen cuvette holder and close the cover to allow dark adaptation.
•
Dark adaptation of the sample is required prior to the following measurements: F
0
, QY, NPQ, LC. Duration of dark-adaptation period
depends on species but mostly varies between 5 and 15 minutes.
•
Mix the sample to avoid sedimentation by holding the AquaPen cover and turning over a few times. This is essential to prevent
inaccurate readings .
•
Turn ON the device – hold SET button for 1 sec.
•
Select Measurement and press SET > select required parameter for example - Ft.
•
Press SET to start the measurement.
•
AquaPen will measure the parameter. If a protocol was selected such as OJIP , LC or NPQ the display will only show the progress of
the measurements in % but no data will be visible.
•
When measuring Ft and QY the value of the parameter will appear on the display after completion of the measurement. To visualize
the data obtained with OJIP, NPQ or LC protocol recorded data has to be download from the AquaPen to the PC computer via USB
cable or the Bluetooth connection using FluorPen Software (page 44).
•
All measured data are stored in the device memory and can be downloaded to PC after completion of the experiment.
Steps for Chlorophyll Fluorescence measurement with AquaPen-P:
•
For measurements of Ft, QY, NPQ, LC the sample requires dark adaptation period of 5-15 min (this varies with species). Place the
sample in the dark to achieve this. Turn ON the device
– hold SET button for 1 sec.
•
Select Measurement and press SET> select required parameter for example - Ft .
•
Submerge the probe in the sample and ensure that no air bubbles get trapped inside the probe.
•
Press SET to start measurements.
•
AquaPen will measure the parameter. If a protocol was selected such as OJIP , LC or NPQ the display will only show the progress of
the measurements in % but no data will be visible.
•
When measuring Ft and QY the value of the parameter will appear on the display after completion of the measurement. To visualize
the data obtained with OJIP, NPQ or LC protocol, recorded data has to be download from the AquaPen to the PC computer via USB
cable or the Bluetooth connection using FluorPen Software (page 44).
•
All measured data are stored in the device memory and can be downloaded to PC after completion of the experiment.
5.1.3 OJIP
PROTOCOL
The AquaPen device offers the protocol to capture rapid fluorescence transient – OJIP, which occurs during exposure of photosynthetic
organisms to high irradiance. The FluorPen software enables data downloading to a PC and subsequent OJIP visualization of the analyzed
data in a graphical and tabular format.