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  Preventing contamination

 

 
To prevent contamination of the chamber, select a suitable installation site. 
 

 Avoid locations with high temperatures or humidity. 

Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in 
the air.   

 

 Avoid locations with passers-by or drafts. 

Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry of 
microorganisms into the chamber. 

 

 If possible, use a cleanroom. 

To achieve a better culture, it is recommended that a cleanroom be used if one is available. 

 

 Use clean containers. 

The greatest cause of contamination is dirty containers for cultures. Be careful not to get containers or 
trays dirty when taking them in and out. 

 

 Keep the chamber clean. 

Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long 
time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a dry 
sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details, 
refer to “ROUTINE MAINTENANCE” on page 86. 

 

 Use sterile distilled water in the humidifying pan. 

Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because it may cause

 

red rust-like particles in the humidifying pan. Clean the humidifying pan once a month. In some cases, an 
antibacterial ingredient may precipitate in the water for humidifying. This is not a malfunction. 
 

 Keep the incubator out of direct airflows from air conditioners or fans. 

Cool airflow from an air conditioner may cause condensation and lead to possible contamination. 
 
 

Summary of Contents for MCO-170AICUVDL

Page 1: ...carefully before using this product and keep the operating instructions for future use See page 96 for model number Operating Instructions CO2 Incubator This product is for research use only Not for...

Page 2: ......

Page 3: ...BETTER CULTIVATION Precautions for cultures 26 Preventing contamination 27 CORRECT OPERATION 28 BASIC OPERATION ON LCD TOUCH PANEL 29 BASIC PARAMETERS Numerical input to input window 31 Setting temper...

Page 4: ...Setting UV lamp ON period 75 Lighting UV lamp for 24 hours 77 GAS AUTO CHANGER OPTION 79 Connecting CO2 gas cylinder 79 Automatic CO2 gas supply line changeover 80 Manual CO2 gas supply line A B chan...

Page 5: ...nctions Contact our sales representative or agent if any page of the operating instructions is lost or the page order is incorrect Contact our sales representative or agent if any point in the operati...

Page 6: ...Symbol shows This symbol means caution This symbol means an action is prohibited This symbol means an instruction must be followed Be sure to keep the operating instructions in a place accessible to...

Page 7: ...and safe disposal options visit ec gc ca mercure mercury Dispose of or recycle in accordance with applicable laws Pour plus de renseignements sur les proc dures de manutention s curitaire les mesures...

Page 8: ...wer supply outlet is not grounded it will be necessary to install a ground by qualified engineers Never ground the unit through a gas pipe water main telephone line or lightning rod Such grounding may...

Page 9: ...pply outlet grip the power supply plug not the cord Pulling the power supply cord may result in electric shock or fire by short circuit Disconnect the power supply plug before moving the unit Take car...

Page 10: ...cted by branch circuit breaker Use a dedicated power source as indicated on the rating label attached to the unit A multiple tap may cause fire resulting from abnormal heating Never store corrosive su...

Page 11: ...h This symbol means ON for a power switch This symbol means OFF for a power switch ENVIRONMENTAL CONDITIONS This equipment is designed to be safe at least under the following conditions based on the I...

Page 12: ...Electric key 10 9 8 18 When 12 is removed Sample air outlet cap 17 16 15 14 13 19 Leveling feet Leveling foot Handles Removable power supply cord port Lower right side When some inner attachments are...

Page 13: ...e door is open The UV lamp is also deactivated by the door opening 11 Key hole This is the hole to unlock with unlock key while outer door is locked by electric lock 12 Switch cover Prevent the accide...

Page 14: ...ensity is set 0 5 Set CO2 density value display field The set value of the chamber CO2 density is displayed Default setting 0 6 CO2 gas injection indicator This indicator lights when CO2 gas is being...

Page 15: ...rs and reverse video Close Door Closed is displayed Locked Door Locked is displayed 13 Message display field Alarms errors or messages are displayed when fault occurs Refer to page 87 90 Note When the...

Page 16: ...tact capacity is DC 30 V 2 A When the Buzzer key is pressed the behavior of the remote alarm is showed in Table 1 Note When the door alarm is activated the remote alarm does not work Refer to page 87...

Page 17: ...rces Do not install the incubator near significant heat sources such as heaters boilers ovens or autoclaves Heat will adversely affect the performance of the incubator Ambient temperature at least 5 C...

Page 18: ...water In addition do not install it near water or steam pipes Moisture can cause the insulation to deteriorate which may result in current leakage or electric shock No inflammable or corrosive gas Ne...

Page 19: ...move the cable tie banding the power supply cord Prolonged banding may cause the corrosion of the cord coating WARNING Do not leave the plastic wrapping bags within reach of children If the bag is pla...

Page 20: ...installed by qualified personnel If the incubator is not grounded it may result in electric shock Never connect the ground wire to a gas pipe water pipe lightning rod or telephone ground wire Doing so...

Page 21: ...simultaneously the surface temperature may be higher than the case of one incubator When stacking the incubators on our CO2 incubator or O2 CO2 incubator other than this product use the stacking plat...

Page 22: ...coming off Make sure that the gas tube is not folded If the CO2 gas is supplied to multiple CO2 incubators from a single gas cylinder a CO2 solid will be formed in the gas regulator The gas regulator...

Page 23: ...cid alkali or chlorine Doing so may cause discoloration corrosion or rusting Be careful to keep the diluted detergent or water out of the temperature sensor the CO2 gas injection port the inner sample...

Page 24: ...asions from sharp edges or corners Be careful not to damage the UV lamp in the duct 1 Turn OFF the power to the incubator 2 Open the outer and inner doors and pull out all the trays Fig 1 3 Pull out t...

Page 25: ...ct and remove it from the pins on the rear side Fig 5 7 Pull out the fan Fig 6 8 Remove the silicon caps of each access port from interior Fig 7 and exterior Fig 8 Duct Silicon cap Fig 5 Fig 6 Fig 7 F...

Page 26: ...le of duct with the projection of fan cover and insert directly Fig 3 Same applies for the humidifying pan cover The fan cover may lean by strongly pushing the head of it in the back Make sure that th...

Page 27: ...ding low temperature water will lower the temperature and humidity in the chamber Install the humidifying pan in a longitudinal direction as its shorter side is placed in the back Refill the humidifyi...

Page 28: ...rs forcefully may cause spillage of the culture medium incomplete closing or damage to the gasket Be careful when closing the outer door Use the handle when closing the outer door Holding the door in...

Page 29: ...em in and out Keep the chamber clean Wipe off any fingerprints If water spills from the humidifying pan or if the doors are left open for a long time condensation may form on the inside of the doors I...

Page 30: ...ly cord that is provided Other power supply cord may cause electric shock or fire The provided removable power supply cord is only for this product Never use it for any other products During dry heat...

Page 31: ...Dry heat sterilisation log can be exported 54 55 Sterilisation Export Export Dry heat sterilisation log 56 57 Lock Lock screen Auto Lock Auto Lock screen Setting Auto lock ON OFF User ID ON OFF 69 71...

Page 32: ...ools 1 screen or press the Top key to return to the Top screen When these settings on this screen are changed inaccurate temperature or CO2 density may be displayed Operation from other than the Menu...

Page 33: ...press the OK key Key description Numerical key 0 9 Input numerical values Up Down key Increases or decreases the numerical value displayed in the numerical input box Clear key Deletes the numerical va...

Page 34: ...the CO2 density and the temperature of the high limit temperature alarm for normal operation according to the following procedure The incubator automatically starts operation using these settings aft...

Page 35: ...ss than 45 0 o C to 45 0 o C or higher the incubator readjusts the CO2 sensor During readjusting Status Gas sensor initializing is displayed in the Message display field and is displayed in the Presen...

Page 36: ...34 BASIC PARAMETERS Setting key lock 1 Press the Menu key to lead the Menu screen 2 Press the Lock key to lead the Lock screen 3 Press the KeyLock key to lead the Key Lock screen...

Page 37: ...Lock turns to ON Password The number Max 6 digit inputted here are registered the release password of Key Lock Confirm Password To prevent erroneous input input the same password as Password input bo...

Page 38: ...nd input of the release password of Key Lock is required When the inputted password is incorrect Notice dialog box is displayed Press the OK key and then input the correct password Removing key lock 1...

Page 39: ...n 5 On the Key Lock screen by holding the Key Lock slide key and sliding to the left change to OFF Press the Apply key to turn the key lock OFF The display returns to the Lock screen Note The release...

Page 40: ...alarm delay ring back and remote alarm go to procedure 5 4 On the Alarm Setting 1 screen input each parameter Press the Apply key to save the input value The display returns to the Tools 1 screen Eac...

Page 41: ...uzzer doesn t sound after the elapse of the set time of door alarm delay Ring Back The function is that the alarm buzzer sounds again when the alarm state still continues after the set time of ring ba...

Page 42: ...e behavior at the time of pressing the Buzzer key and the re activation of alarm under each setting condition refer to Table 5 7 on page 88 Resolve the cause of the alarm in reference to page 87 90 be...

Page 43: ...ration log data chamber temperature CO2 density and open close state of outer door Use the following procedure to set the log interval interval of acquiring the operation log 1 Press the Menu key to l...

Page 44: ...n between log interval and the estimated amount of data that can be saved Log interval 2 minutes Approx 46 days Log interval 6 minutes Approx 135 days Log interval 30 minutes Approx 664 days When savi...

Page 45: ...phically 5 On the Chart screen by pressing the Show key after pressing the item you want to display graphically the graph of each operation log is displayed Actual Temp Chamber temperature log graph G...

Page 46: ...alizing of the Gas sensor or during dry heat sterilisation Therefore the CO2 density log data may be different from the true value 8 Door Opening log graph is displayed Press the Back key to return to...

Page 47: ...memory into the USB port Note It is not possible to use a USB memory with security functions that requires entering password 10 Press the Export key 11 When the export is complete Information dialog b...

Page 48: ...t to the USB memory inserted into the USB port 1 Insert the USB memory into the USB port Note It is not possible to use a USB memory with security functions that requires entering password 2 Press the...

Page 49: ...og data press the All Ch key To export only operation log data you want to export select operation log data you want to export and then press the Selected Ch key Actual Temp Chamber temperature log da...

Page 50: ...22Jun2016_Temp csv Actual CO2 Level The oldest date of Actual CO2 Level log date of that day_CO2 20141013 20160622_CO2 csv 13Oct2014 22Jun2016_CO2 csv Door Opening The oldest date of Door Opening log...

Page 51: ...log saved in the incubator can be displayed graphically on the LCD touch panel Note When saving alarm logs more than 257 the oldest alarm log is deleted and then overwritten 1 Press the Menu key to l...

Page 52: ...e range 1 day 45 days Note The error of about 1 minute may be observed during 1 month Refer to page 59 60 for the procedure of setting time Press the Back key to return to the Log screen Press the Top...

Page 53: ...port by CSV format 1 Insert a USB memory in the USB port Note It is not possible to use a USB memory with security functions that requires entering password 2 Press the Menu key to lead the Menu scree...

Page 54: ...io button and input days Settable range 1 day 45 days Note The error of about 1 minute may be observed during 1 month Refer to page 59 60 for the procedure of setting time 6 Press the Export key Note...

Page 55: ...exported data file is saved in it by CSV format The exported file name depends on the date format at the time of export refer to page 59 60 Exported file sample All Last xx Days The oldest date in th...

Page 56: ...ogs Sterilisation logs saved in the incubator can be displayed graphically on the LCD touch panel Note When saving sterilisation logs more than 251 the oldest alarm log is deleted and then overwritten...

Page 57: ...Note The error of about 1 minute may be observed during 1 month Refer to page 59 60 for the procedure of setting time Press the Back key to return to the Log screen Press the Top key to return to the...

Page 58: ...erted in the USB port by CSV format 1 Insert a USB memory in the USB port Note It is not possible to use a USB memory with security functions that requires entering password 2 Press the Menu key to le...

Page 59: ...input the number of logs Settable range 1 log 250 logs Note The error of about 1 minute may be observed during 1 month Refer to page 59 60 for the procedure of setting time 6 Press the Export key Not...

Page 60: ...it by CSV format The exported file name depends on the date format at the time of export refer to next page Exported file sample All Last xx Logs The oldest date in the stored sterilisation log the l...

Page 61: ...lead the Tools 2 screen 3 Press the Date Time key to lead the Date Time screen 4 On the Date Time screen select the date format To display the date in the order of year month and day press the Year Mo...

Page 62: ...24 hour clock It is recommended to set the time periodically since the error of about 1 minute may be observed during a month 6 Press the Top key to return to the Top screen Setting brightness and sl...

Page 63: ...no key operation during set time By holding the Sleep slide key and sliding it right the Sleep function is turned to ON Input the set value of time to change the Sleep state Settable range 1 minute 5...

Page 64: ...lisation make sure that the outer and inner doors are securely closed During dry heat sterilisation plug the access hole with the silicon caps that are provided Failure to do so may cause burns Dry he...

Page 65: ...other objects such as dishes or flasks 9 Set the humidifying pan on the top tray Refer to the Before Sterilisation Operation screen 10 Close the inner door and the outer door Note Make sure that the i...

Page 66: ...Step3 screen and dry heat sterilisation is started Dry heat sterilisation is performed automatically from Step3 to Step6 procedure 14 Press the Cancel key to return to the Top screen Note The outer d...

Page 67: ...C or higher for 60 minutes sterilisation process Note By pressing the Abort key in step3 or step4 dry heat sterilisation is stopped in the middle of dry heat sterilisation and goes to Step5 the cooli...

Page 68: ...error displayed in the message display field in reference to Table 8 on page 89 90 Press the OK key to return to the Top screen and perform dry heat sterilisation again Note This screen may be displa...

Page 69: ...the Unlock key User ID mode Input the User ID and release password of Auto Lock after pressing the Unlock key Setting User ID Before turning the User ID mode to ON use the following procedure to regis...

Page 70: ...password as Password input box When inputting different password Notice dialog box is displayed Press the OK key and input the correct password Note A release password of Auto Lock is for unlocking th...

Page 71: ...into User ID input box and input its registered password into Password input box Press the Delete key to delete the registered User ID and its password Note When deleting all registered User IDs the...

Page 72: ...mode of unlocking the outer door between the quick mode or the User ID mode By holding the User ID slide key and sliding it right the User ID mode is turned to ON Factory setting OFF quick mode Note W...

Page 73: ...s release password of Auto Lock Note The User ID which is inputted at this time is saved as the open close state of outer door log data refer to page 46 47 When the inputted User ID or its password is...

Page 74: ...o the lock direction while the outer door is open Close the outer door after the out door is locked manually Note The outer door cannot be locked by using the unlock key while the outer door is closed...

Page 75: ...ding the Auto lock slide key and sliding it left the Auto lock is turned to OFF Press the Apply key to change Auto lock OFF and the display returns to the Lock screen Note When removing Auto lock duri...

Page 76: ...OFF Then when the door is closed the lamp will light for the preset period If only the outer door is repeated opened and closed it may have a bad influence on the condensation in the chamber and chamb...

Page 77: ...sentative or agent for information on replacing the UV lamp If the UV lamp burns out Err18 UV Lamp Abnormal is displayed in the message display field If this occurs replace the UV lamp When replacing...

Page 78: ...p has turned on is displayed as the percentage to 5 000 hours which are recommendation time to replace It is impossible to set UV Timer Ext The more total time which UV lamp has turned on increases th...

Page 79: ...fan cover the duct the fan the humidifying pan and the humidifying pan cover If possilbe autoclave them for sterilisation otherwise clean and wipe off them with alcohol for disinfection 2 Clean and wi...

Page 80: ...condition display when UV lamp is lighting Note The UV 24 hour mode may cause the automatic set temperature alarm because of a rising chamber temperature After procedure 8 by opening the outer door w...

Page 81: ...ting port B for CO2 gas pipe and the gas regulator of the CO2 gas cylinder B Note If the CO2 gas is supplied to multiple CO2 incubators from a single gas cylinder a CO2 solid will be formed in the gas...

Page 82: ...anger MCO 21GC is installed CO2 gas supply line indicator A B and the CO2 gas supply line select key are displayed in the Top screen The CO2 gas supply line indicator A or B used now lights CO2 gas su...

Page 83: ...into CO2 gas cylinder A Table 2 CO2 gas supply line automatic changeover e g When CO2 gas cylinder A is empty it changes over CO2 gas cylinder B Situation CO2 gas CO2 gas supply line indicator Message...

Page 84: ...CO2 gas supply line A is changed to B by CO2 gas automatic changer function the CO2 gas supply line B is changed to A manually without pressing the Buzzer key Table 3 Situation CO2 gas CO2 gas supply...

Page 85: ...bator Since a Standard gas cylinder is used as a standard of exact density during CO2 density calibration prepare a standard gas cylinder that is same as the set CO2 density Note There is not the prob...

Page 86: ...to the Tools 1 screen Settable range 4 0 21 0 Factory setting 5 0 6 Press the STD Gas Calibration key to lead the STD Gas Calibration screen 7 On the STD Gas Calibration Step1 screen system check star...

Page 87: ...tomatically 10 After completion of CO2 density calibration display leads the STD Gas Calibration Step5 screen CO2 incubator returns to the normal operation 11 On the STD Gas Calibration Step5 screen p...

Page 88: ...upply before maintenance The outer door is locked when the power is disconnected before opening the outer door 3 Remove all the inner attachments by the procedures shown on page 22 4 Clean the chamber...

Page 89: ...ened Err01 CO2 Gas Empty is displayed in the message display field Chamber temperature sensor error The chamber temperature sensor is disconnected Err05 Temp Sensor Open is displayed in the message di...

Page 90: ...ation OFF OFF OFF Interlock with the Buzzer key ON ON OFF Alarm is not canceled ON OFF OFF OFF Note Resolve the cause of the alarm in reference to page 87 90 because the alarm itself is not deactivate...

Page 91: ...terilisation step5 Sterilisation step6 4 Sterilisation Finished Successfully Press OK key to end Saved Success 4 During sterilisation process Step4 During a power failure The power switch is OFF The r...

Page 92: ...error is canceled After recovery from power failure Err51 Power Failure Intermittent tone ON Saved Success The outer door is opened The electric lock is unlocked Err52 Door Lock Failure Continuous ton...

Page 93: ...8 kgf cm2 G 551 psi G or lower it is a sign that there is little gas remaining Replace the cylinder soon Is the incubator operating beside the appliance that generates the electromagnetic wave The cha...

Page 94: ...Is the secondary pressure for the gas regulator at the specified value of 0 03 MPa G 0 1 MPa G 0 3 kgf cm2 G 1 kgf cm2 G 4 4 psi G 14 5 psi G Is the gas tube blocked Is the duct securely attached Att...

Page 95: ...e CO2 incubator is left where outsiders enter it may result unexpected accident for example children to become locked inside Before disposing the CO2 incubator with biohazardous danger decontaminate t...

Page 96: ...th humidifying pan Temperature controller PID control system Temperature display Digital display CO2 controller PID control system CO2 density display Digital display Air circulation Fan assisted Air...

Page 97: ...ace board MTR 480 For RS 232C RS 485 Only for the Data acquisition system MTR 5000 user Note Refer to the updated catalog when ordering an optional component Designs and specifications are subject to...

Page 98: ...5 R H 5 R H Applicable environment condition Temperature 5 o C to 35 o C Humidity 80 R H max The designed performance may not be obtained if the ambient temperature is equal or less than 15 o C or if...

Page 99: ...the unit Unit interior No contamination Decontaminated Contaminated Others 3 Instructions for safe repair maintenance disposal of the unit a The unit is safe to work on b There is some danger see bel...

Page 100: ...Printed in Japan LDCL040500 0 S0418 0 1 1 1 Sakada Oizumi machi Ora gun Gunma 370 0596 Japan PHC Corporation 2018...

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