PerkinElmer Wallac 1420, User Manual

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Fluorescence polarization (Wallac 1420-040/041/042/043 only)
Fluorescence polarization - light of a particular wavelength is selected with a filter from the
spectrum of a continuous wave lamp. This light is then polarized and used to excite the
fluorochrome in the sample. The emission light is then viewed through two polarizers, one
parallel to the incident polarization (S-plane) and one perpendicular to it (P-plane). These
are, each in turn, moved automatically into the emission light path. The ratio:
1000*(Parallel –G*Perpendicular) / (PaG*Perpendicular)
is calculated to get the polarization in units of mP. G is a correction factor, see the end of
this section. The parameters that can be set for fluorescence polarization are the following:
Protocol name
This is the name by which the protocol is identified. A password can be associated with it to
prevent parameters being changed by an unauthorized person.