PerkinElmer Spotlight 200, User Manual, Page: 87 / 132

Techniques for Collecting Spectra . 87
Removing the ATR Crystal Holder from the
Microscope
To use the microscope for conventional microspectroscopy, you need only retract the ATR
crystal from its working position. If, however you need a very large working distance, for
example if the sample is recessed, the ATR crystal holder can be removed.
1. Switch off the microscope at the electricity supply.
2. Stop any laser radiation from entering the microscope by switching the internal beam of
the spectrometer to the internal sample compartment.
See the Spectrum on-screen Help for further information.
3. Make sure that the ATR crystal is in the raised position.
4. Screw the plastic protective cover in place, over the crystal.
5. For the automated ATR objective, remove the jack plug from its socket.
6. While holding the crystal assembly, unscrew the two adjustment thumb-nuts
completely. Allow the assembly to drop vertically from the cassegrain. Be careful not to
disturb the two adjusting levers.
Any movement of the adjusting levers will change the alignment of the assembly when
it is refitted.
7. Store the assembly carefully for future use.
When refitted, the assembly will still be aligned, provided that the adjusting levers have
not been moved.
8. Direct the infrared beam back to the microscope.