PerkinElmer Spotlight 200, User Manual, Page: 46 / 132

46 . Spotlight 200 User’s Guide
Setting up the Spotlight 200
To set up your Spotlight 200:
1. Make sure the spectrometer is switched on.
It can take the spectrometer up to two hours to equilibrate after being switched off
overnight.
2. Switch on the microscope at the rear switch.
The blue LED light at the front comes on.
3. Switch on the stage controller.
The green LED light at the front comes on.
4. At your PC, click
Start
and then select
Spectrum
in the
PerkinElmer Applications
group under All Programs .
Spectrum software starts.
5. Enter your
User name
and, if required, your
Password
.
6. Select the spectrometer connected to your Spotlight 200.
7. Select Instrument from the Setup menu, and click the Setup Instrument BeamPath
tab.
8. Click the microscope detector to direct the beam to the microscope.
The beampath diagram and accessory toolbar are updated to show that the microscope
is connected.
9. Click the microscope button on the accessory toolbar.
10. Make sure that the lower cassegrain is fitted, that the ATR objective crystal (if fitted) is
retracted, and that there is no sample on the stage, and then click Start .
The microscope initializes.
NOTE: If you want to avoid initializing the microscope at the start of a session, click Skip to
apply the settings from the previous session. This option is only available if the
microscope has previously been initialized.
Focusing the Microscope
The focus of the microscope is changed by moving the sample stage up or down. The
Z
-control on the joystick, or the
Auto-Focus
button on the Setup Microscope Basic tab,
enables you to focus the image in the Camera View.
The default position for the lower cassegrain gives optimal illumination for thin samples. For
optimal performance it may be necessary to refocus the lower cassegrain to compensate for
the sample’s refractive index.