PerkinElmer Spotlight 200, User Manual

Page: 71 / 132

Techniques for Collecting Spectra . 71
Purging the system
1. Make sure that all parts of the purge system, as listed above, are in place.
2. Set up the microscope.
See
Getting Ready to Use the Spotlight 200
starting on page 41.
3. Place the sample in position.
4. Purge the microscope and spectrometer for 15 to 20 minutes at a rate of
10 l min
− 1
.
5. Collect the background spectrum and the spectrum of the sample.
See the on-screen
Help for further information about collecting spectra.
Changing the sample
If you have changed a sample, any air that has entered the system must be flushed out.