PerkinElmer Spotlight 200, User Manual

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Overview of the Spotlight 200 . 35
Visible light optics in transmittance
When the microscope is in viewing mode in transmittance (Figure 10):
• The lower fold mirror, beneath the cassegrains, receives light from the LED source and
directs it up through the lower dichroic mirror onto the lower cassegrain.
• The lower cassegrain condenses the beam to an appropriate size for a microscopic
sample and focuses it at the sample position.
•
The upper cassegrain collects light from the sample and sends it upward through the
aperture and the upper dichroic mirror.
Figure 10 Path of the Visible Beam for Viewing a Sample in Transmittance
NOTE: Figure 10, above, illustrates a Spotlight 200 with an MCT detector. Other detector
types may look different, but the optical arrangement is the same in all cases.
Detector
Camera
Mirror pair
Visible focus
lens assembly
Dichroic mirror
Variable aperture
Upper cassegrain
Sample position
Lower cassegrain
Lower dichroic
mirror
Lower fold mirror
Lower mirror assembly
LED