PerkinElmer RamanStation 400, Getting Started Manual

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102 . RamanStation 400 Series Getting Started Guide
5. Select Validate and then Instrument from the Instrument menu in Spectrum.
The Validation / Calibration dialog is displayed.
6. Select the Calibration tab.
7. Select Laser Frequency, and then click OK.
The instrument will now perform the laser frequency calibration routine. This will take a
few minutes.
After completing the laser frequency calibration routine, a report is generated on-screen
that gives details on the outcomes of the laser frequency calibration.
In the unlikely event that the laser frequency calibration routine should fail, please perform
a wavelength calibration and repeat the laser frequency calibration.
The instrument in now laser frequency calibrated.
SOP-13: Intensity Calibration of the
RamanStation 400
The intensity calibration must be performed using the National Institute of Standards and
Technology (NIST) Standard Reference Material. NIST SRM 2241 is the relative intensity
correction standard for Raman spectroscopy when using 785 nm excitation.
Intensity calibration should typically be performed on an annual basis, that is during an
annual instrument service, or whenever the instrument is moved.
NOTE: As intensity calibration is not required on a regular basis, NIST Standards are not
included in the basic RamanStation 400 package. Standard Reference Materials can
be purchased from PerkinElmer (L1321831). Your instrument will be intensity
calibrated prior to shipping or during installation.
NOTE: If you have a fiber optic probe, you must perform an intensity calibration on both the
sample compartment and the fiber optic probe.
1. Ensure the RamanStation 400 is switched on, if not follow SOP-1.
2. Open the Scan and Instrument Setup dialog, and on the Hardware tab ensure that the
laser power is set to 100%.
3. In the Scan and Instrument Setup dialog, click Spectral Preview and then focus on
the appropriate NIST standard.
When the NIST standard is optimally focused, close the dialog by clicking Exit.
4. Select Validate and then Instrument from the Instrument menu in Spectrum.
The Validation / Calibration dialog is displayed.
5. Select the Calibration tab.