PerkinElmer RamanMicro 200 Series, Getting Started Manual

Page: 70 / 98

70 . RamanMicro 200 Series Getting Started Guide
17. Focus on the sample and again move the sample in the
x
and
y
directions, if necessary.
The crosshairs indicate the spot where analysis takes place.
NOTE: If there is an offset between the laser position and the cross hairs, use the
Align
Video with Laser
tool to adjust this. See the Spectrum on-screen help and
Aligning
the Video Camera with the Laser Spot
on page 52 for more information.
18. When the sample is correctly positioned, switch off the illumination source.
19. Select the Dark Field (DF) switch position on the microscope.
20. If the microscope light-proof enclosure is fitted, close the doors.
21. Click .
The RamanMicro 200 will start collecting data in the Live display.
You can use Monitor to help you optimize the Exposure time and Laser power for your
sample, and adjust the focus of your sample to maximize the Raman signal. The data
displayed on-screen when monitoring cannot be saved. To save data you must collect
spectra.
Click to exit the Live display.
You are now ready to acquire data. For more information see SOP-6.
The most appropriate microscope objective for your analyses will depend upon your samples.
High-magnification objective lenses give the most efficient Raman collection from thin
samples, such as films or wafers, but low-magnification objectives are better for macro
samples such as tablets, bulk plastics and tissue samples.
NOTE: Raman spectra are measured in the visible and near-infrared spectrum. Room
lighting and sunlight will contaminate your spectra. Take care not to misinterpret
room lighting or sunlight features as sample features.
SOP-5: Sample Positioning using a Motorized Sample
Stage
WARNING
When you are using a motorized stage, do not place your fingers
between the moving and fixed parts of the stage. The motors driving
the stage are powerful and do not stall easily.
1. Select the 5x objective lens on the microscope.
2. Select the Bright Field (BF) switch position on the microscope.
3. Switch on the illumination source.
4. Select
Raman Instrument
from the Setup menu.
The Setup Instrument tabs are displayed.