PerkinElmer RamanMicro 200 Series, Getting Started Manual

Page: 51 / 98

Installing Your RamanMicro 200 Series . 51
9. Insert the adjuster keys supplied with the microscope into the nosepiece and move the
adjusters until the crosshairs are aligned with the same feature as for the previous
objective lens.
Figure 22 Microscope objective adjusters
10. Repeat Steps 8–9 until all the objectives are aligned.
Figure 23 Feature in center of cross hairs at three objective magnifications
Aligning the Raman Module
There are three levels of optical alignment in the Raman module:
(i) Alignment of the laser path
(ii) Alignment of the video camera
(iii) Alignment of the Raman coupler
Level (ii) and level (iii) may be carried out without removing any instrument paneling. Level
(i) requires removal of instrument paneling and must only be carried out by a PerkinElmer
Service Representative, and in a Class 3B environment.
Aligning the Laser Path
The laser path only needs realignment if the beam is observed to move grossly in the
xy -plane when moving into and out of focus. If the laser path is aligned correctly, then the
laser spot should get larger in off-focus positions without appreciable lateral movement.
NOTE: The requirement for laser alignment is NOT indicated by a situation where the laser
spot and the center of the crosshairs are non-coincident (this scenario requires
realignment of the video camera with the laser spot).