PerkinElmer AVIO 200, Hardware Manual

Page: 254 / 309

Maintenance
252
Adjusting the Pump Tubing
Adjust the tension on the sample and drain tubing, one at a time, using deionized
water as follows:
Adjusting the Sample Tubing
1. Make sure the sample tubing is centered in the channel, then place the clamp
around the tubing and lower the clamp arm on onto the pump. Turn the
adjustment screws until locked in place.
2. Place the sample capillary (SAMPLE IN side) in a container of deionized water.
3. Set the pump speed to 1.0 mL/min and start the pump.
4. Dip the tubing in DI water a few times to let air gaps form. As you watch the air
gaps form loosen the adjustment screw in a counterclockwise direction until the
air gaps stop or do not flow smoothly.
5. Tighten the adjustment screw slowly (turn clockwise) until droplets just start
forming at the end of the SAMPLE OUT side of the sample tubing.
6. Tighten the adjustment screw until you see a smooth flow of bubbles (3 turns on
the peristaltic pump). Add an additional three more turns.
7. Turn off the pump. The tension is now correctly set. No bubbles should be seen
in the sample tubing once the sample tube is kept in the solution.
Adjusting the Drain Tubing
1. Make sure the drain tubing is centered in the channel without disturbing the
sample tubing, then place the clamp around the drain tubing and lower the clamp
arm.
2. Loosen the adjustment screw (turn counterclockwise) for the drain tubing
channel so that the lever can be easily opened and closed with little resistance.
3. Set the pump speed to 1.0 mL/min and start the pump.
4. Dip the tubing in DI water a few times to let bubbles form. As you watch the
bubbles form loosen the adjustment screw in a counterclockwise direction until
the bubbles stop or do not flow smoothly.