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Perkin-Elmer RamanStation 400 Series, Getting Started Manual

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64 . RamanStation 400 Series Getting Started Guide 

Acquiring Spectra with the Fiber Optic Probe 

The standard Raman fiber optic probe has a tight focus spot that is approximately 7.5 mm 

away from the lens. The focused spot enables you to pinpoint the measurement area on the 

sample. 

Sampling Solids 

Solids can be analyzed by mounting the probe on a stand and adjusting the focal spot on the 

surface of the sample. Whenever possible, powders should be compacted down and thin 

films of materials should be folded so that the sample covers the entire depth of field. Solids 

can be analyzed in glass and plastic containers, similar to liquid samples. 

NOTE: Raman spectra are measured in the visible and near infrared region. Room lighting 

and sunlight will contaminate your spectra. Take care not to misinterpret room 

lighting or sunlight features as sample features. 

 

If using a RamanStation 400F, and a fiber optic probe or RamanMicro 300 Accessory 

is connected, ensure that the probe or microscope objectives are covered (or, if 

fitted, that the microscope enclosure is closed) and that the microscope illumination 

is off and the switch is set to Dark Field (DF) when using the sample compartment to 

prevent contamination. 

Sampling Liquids 

Liquid samples can be analyzed by immersing the FOP in the liquid of interest or by analyzing 

through the container.  

Immersion

 

The FOP can only be immersed in liquid if you have purchased an immersible fiber probe 

sleeve. Do not totally submerge the probe. It is only acceptable to immerse the probe up to 

1 inch from the top of the sleeve. It may be useful to focus the sample by sliding the FOP 

inside the immersion sleeve to obtain the best spectrum. Note that immersion sleeves 

contain either quartz or sapphire windows, these windows can yield a detailed Raman 

spectrum – do not confuse this with the spectrum of the sample. 

 

Figure 31 Fiber Optic Probe with the immersion sleeve detached

 

Immersion sleeve 

Fiber optic probe 

Summary of Contents for RamanStation 400 Series

Page 1: ...RamanStation 400 Series Getting Started Guide MOLECULAR SPECTROSCOPY ...

Page 2: ...tability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoeve...

Page 3: ...ation 27 Raman Theory 28 The RamanStation 400 Series 30 Spectrograph Detector Systems 32 Preparing Your Facility 35 Specifications 36 Spectrometer 36 Stage Controller 36 Electricity supply 36 Finding a Suitable Location for the Instrument 37 Instrument Dimensions 38 Installing Your RamanStation 400 Series 39 Unpacking the Instrument 40 Lifting the Instrument 42 Preparing the Motorized XYZ Stage 43...

Page 4: ... Optic Probe SOPs 78 SOP 6 Selecting the Fiber Optic Probe 78 SOP 7 SamplePositioningwhen usingaFiberOpticProbe 79 Calibration and Performance Verification SOPs 80 SOP 8 Calibration Performance Verification 80 SOP 9 Wavelength Calibration Verification 81 SOP 10 Wavelength Calibration 83 SOP 11 Laser Frequency Calibration 84 SOP 12 Intensity Calibration 85 Summary 87 Additional Parts and Upgrades 8...

Page 5: ...Introduction ...

Page 6: ...RamanStation 400 Series instrument and what utilities are required for example electricity and temperature control Installing your RamanStation 400 Series This chapter contains step by step instructions about how to unpack and assemble your RamanStation 400 Series instrument and information on installing your instrument in the software Introduction to the RamanStation 400 Series This chapter descr...

Page 7: ...e to press two keys at the same time for example ALT F All eight digit numbers are PerkinElmer part numbers unless stated otherwise The term RamanStation 400 Series means the RamanStation 400 and the RamanStation 400F Raman Spectrometers Notes Cautions and Warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings NOTE A note indicates addi...

Page 8: ... otros En los recuadros como éste se proporciona información sobre este tipo de circunstancias Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoquer de graves détériorations de l instrument ou d autre matériel Les détails sur ces circonstances figurent dans un encadré semblable à celui ci Caution Attenzione Con il termine CAUTION ATTENZI...

Page 9: ...recuadros como éste se proporciona información sobre este tipo de circunstancias Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages corporels à l utilisateur ou à d autres personnes Les détails sur ces circonstances sont données dans un encadré semblable à celui ci Warning Pericolo Con il termine WARNING PERICOLO vengono segnalate s...

Page 10: ...10 RamanStation 400 Series Getting Started Guide ...

Page 11: ...Safety Practices ...

Page 12: ...elieved to be correct and complete PerkinElmer shall not be liable for errors contained herein nor damages concerned with the use of this document PerkinElmer does not accept liability for the interpretation of information resulting either directly or indirectly from our spectrometers or associated software interfaces All results supplied by PerkinElmer s spectrometers or software are intended to ...

Page 13: ...erature 15 C to 30 C Relative Humidity 80 maximum non condensing Avoid any adjustment maintenance or repair of the opened operating instrument If any adjustment maintenance or repair of the opened instrument is necessary this must be done by a skilled person who is aware of the hazards involved The instrument has been designed to be safe under the following conditions Indoor use Altitude up to 200...

Page 14: ...n 400 Series Getting Started Guide Fire Safety Ensure that there is at least 15 cm 6 between the rear of the instrument and the nearest wall Ensure that any power supply unit PSU vents or fan do not become obstructed ...

Page 15: ...d disconnect them from the line power supply before you alter or make any new electrical connections Electrical Protection Insulation Class I rating for external circuits Only connect equipment that meets the requirements of IEC 61010 1 IEC 60950 or equivalent standards Power Supplies The RamanStation 400 Series instruments and the stage control box for motorized XYZ stage only are each powered by...

Page 16: ...ent and at any time the instrument is plugged into a different power point socket Power Cords Between PSU and Power Outlet Socket The power cords supplied to connect the instrument PSUs to the power points sockets should be appropriate for the country listed as the shipping destination WARNING If the power cord is not appropriate for your country it must be removed from use immediately and replace...

Page 17: ...s with the EMC standard EN61326 EMC standard for electrical equipment for measurement control and laboratory use and EN55011 ISM Class A RF emissions FCC rules and regulations This product is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Sect...

Page 18: ... instrument may be regarded as a Class 1 laser product For more information see Attaching the Fiber Port Cover Assembly on page 46 WARNING Avoid direct exposure to the laser beam Do not look at the laser light or scattered laser light Class 3B Safety Precautions When using the fiber optic probe you should follow Class 3B laser safety precautions such as wearing laser safety goggles and using door ...

Page 19: ...ce s remote interlock connector should be connected to an interlock switch at the entrance For certain laser systems the laser safety officer may require the shutter to be interlocked with the laboratory entrance in order to prevent inadvertent exposure of persons entering to very hazardous laser radiation 4 Rooms in which Class 3B devices are used should have notices affixed to the doors so that ...

Page 20: ...CT EXPOSURE TO THE BEAM Figure 1 Schematic of the fiber optic probe not to scale When using the fiber optic probe the following precautions must always be observed Use an interlock switch on the door to the laboratory and or the reaction vessel when you are using a fiber optic probe Never look directly into the laser beam or at the laser light scattered from a reflective surface or sample Avoid di...

Page 21: ...lled access areas limited to individuals trained in the safe operation of lasers are suggested for laser operations When the probe is in use always ensure that no stray laser radiation is directed at people in the vicinity Use protective laser safety goggles with an optical density OD of at least 3 at 785 nm as a precaution against accidental exposure to the direct or reflected laser light Suitabl...

Page 22: ... exposure can be calculated from 4 7 0 2 10 10 1 10 λ MPE J cm 2 Where λ is the laser wavelength in micrometers µm For example a laser wavelength of 785 nm 0 785 µm will have an MPE value of 1 49 10 2 J cm 2 or 1 49 10 3 W cm 2 for a 10 second exposure or longer Table 2 shows the calculated nominal ocular hazard distance for 785 nm Raman systems Table 2 Nominal Ocular Hazard Distance NOHD for 785 ...

Page 23: ...dered highly dangerous compromising the safe operation of the instrument This type of action is deemed to be a deliberate attempt to bypass the internal instrument safety interlocks and as a consequence of this the instrument will be classified as Class 3B Figure 3 The RamanStation 400 Series safety interlock system showing the proximity of the MPS and MA when the sample lid is closed Note that th...

Page 24: ...anMicro 300 Accessory or systems being used in a Class 3B fashion Fiber optic probes and the RamanMicro 300 Accessory are Class 3B laser products and have the potential to cause harm or injury to those using them or others in the vicinity of the spectrometer or associated fiber optic probe For more information refer to Attaching the External Interlock on page 45 ...

Page 25: ...mW PULSE DURATION CONTINUOUS WAVE EMITTED WAVELENGHS 785nm THIS LASER PRODUCT CONFORMS TO IEC 60825 1 1993 21 CFR 1040 10 1040 11 LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT MAXIMUM OUTPUT 250mW PULSE DURATION CONTINUOUS WAVE EMITTED WAVELENGHS 785nm THIS LASER PRODUCT CONFORMS TO IEC 60825 1 1993 21 CFR 1040 10 1040 11 LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUC...

Page 26: ...on the Exterior of the RamanStation 400 The following labels are displayed on the exterior of the instrument Figure 5 Labels on the RamanStation 400 spectrometer CLASS 1 LASER PRODUCT CLASS 1 LASER PRODUCT Power Max 100W DC Voltage 5V 12V Current Max 4A 6 5A ...

Page 27: ...Raman History Theory and Instrumentation ...

Page 28: ...y scattered and will be detected at either longer wavelengths lower frequencies ν Stokes scattering or shorter wavelengths higher frequencies ν Anti Stokes scattering With Stokes scattering some of the incident photons give up a portion of energy to the molecules and are detected at a lower frequency longer wavelength than the Rayleigh radiation Figure 7 Figure 7 Rayleigh Stokes and Anti Stokes sc...

Page 29: ...ency shifts from the frequency of the incident beam are displayed on the x axis of a Raman spectrum generally in relative wavenumbers relative to the laser wavelength otherwise known as Raman Shift and the intensity of the bands are displayed in arbitrary units on the y axis Figure 8 Raman spectrum of cyclohexane showing Stokes anti Stokes and Rayleigh scattering Anti Stokes x12 Rayleigh Stokes Ra...

Page 30: ...or a RamanMicro 300 Accessory Figure 9 The RamanStation 400 Series The instrument Figure 9 is composed of a laser mirrors filters lenses spectrograph and a CCD detector The laser supplies light through an objective lens onto the sample through the sample compartment snout or through the fiber optic probe The scattered light from the sample is collected with the same objective lens and transmitted ...

Page 31: ... optical head optical layout To Spectrograph From Laser Laser Collimation Lens Bandpass Filter Mirror Lens Rayleigh Filters Beampath Assembly Video Camera Beam Splitter Objective Lens Sample Optical Head detailed block diagram below Laser High Performance TE cooled CCD Detector Spectrograph Motorized XYZ Stage Video Head ...

Page 32: ...ontains two components the Echelle spectrograph and a two dimensional CCD detector Figure 13 The system works by dispersing the light in two dimensions by using two gratings The first grating disperses the light horizontally onto the second grating where the light is further dispersed vertically onto the CCD detector The light is spread over a number of strips on the CCD that are read simultaneous...

Page 33: ...full spectral range There are no moving parts in the spectrograph which improves instrument reliability ruggedness and the quality of calibration The absence of moving parts also means that as you acquire the spectrum there is no change in the spectrograph s stray light performance meaning that there are no stitch marks in the final spectrum No light is lost all the light goes onto the detector an...

Page 34: ...34 RamanStation 400 Series Getting Started Guide ...

Page 35: ...Preparing Your Facility ...

Page 36: ...r fluctuations or brown outs may damage the spectrometer RamanStation 400 RamanStation 400F Weight 45 kg 45 kg Height 490 mm 490 mm Width 590 mm 590 mm Depth 640 mm 640 mm Laser Class Class 1 Class 3B Operational Temperature 15 C 30 C Recommended Temperature 20 C 25 C Power Supply 100 230 V AC 10 50 60 Hz Stage Controller for motorized XYZ stage only Weight 2 8 kg Height 90 mm Width 220 mm Depth 1...

Page 37: ... work comfortably Use conducting floor coverings to minimize static discharge Floor vibrations or noise from heavy manufacturing equipment can affect the performance of the instrument Intense magnetic fields should be at least 5 5 meters 18 feet away from the spectrometer Never place the instrument near a window that gets direct sunlight If the instrument is equipped with a fiber optic probe or a ...

Page 38: ...1 0 kg Laboratory benching must be strong enough to support the combined weight of the instrument and samples typically 46 kg without warping or wobbling The bench must be at least 640 mm 25 in depth There must be at least 400 mm 16 above the instrument to accommodate the open sample compartment lid Figure 14 Dimensions of the RamanStation 400 Series 590 mm 640 mm 490 mm ...

Page 39: ...Installing Your RamanStation 400 Series ...

Page 40: ...f there is any damage do not continue with the installation Contact your PerkinElmer Service Representative immediately Part Number Description RamanStation 400 Series Spectrometer LX108870 Spectrum Std for Raman Software Kit Contains the Spectrum Std Software CD LX100877 the Raman Instrument Configuration Disk LX108869 the Raman Spectral Libraries CD L1321877 and the Spectrum Insight Software CD ...

Page 41: ...ck L1865749 RS 232 cable LX108897 SpectrumIMAGE Software Kit systems with motorized stages only If you have a deep depletion CCD installed in your RamanStation 400 L1320032 L1320033 L1320034 L1320035 you will also receive L1323525 Deep Depletion PSU L1323010 Recirculating Cooler L1321674 Tygon Tubing NOTE Your instrument will be assembled and tested by a PerkinElmer Service Engineer There are no u...

Page 42: ...roper lifting techniques WARNING To lift and or move the instrument safely requires two people WARNING Do not lift the RamanStation 400 Series using the instrument console located on the left hand side of the instrument Always lift the instrument using the positions under the instrument base as shown in Figure 15 Figure 15 Appropriate lifting points on the RamanStation 400 Series ...

Page 43: ...alling the motorized XYZ stage Open the sample compartment of your RamanStation Place the stage on the base of the sample compartment The stage slots into two locator pins protruding from the base The stage is then secured to the base of the sample compartment using two thumbscrews Connect the serial cable coming from the stage to the Sample Accessory port at the back of the sample compartment Con...

Page 44: ...ropriate Figure 18 Figure 18 XYZ connector cable 3 Connect the single end of the same cable to the Sample Accessory port on the back of the spectrometer 4 Connect the RS232 cable L1865749 from the COM1 port on the PC to the socket labeled RS232 on the rear panel of the stage controller 5 Connect the power cable from the stage controller box to the mains supply Do not switch on the stage controller...

Page 45: ...t data even if data is being collected using the fiber optic probe You must attach the External Interlock Override L1320264 into the 7 way DIN connector labeled EXT INTERLOCK on the rear panel of the instrument If your laser safety officer determines that an interlock is required on the door to the room you will need to purchase the Raman Laser Safety Kit L1320262 For more information contact your...

Page 46: ...ed to the instrument The fiber optic probe cover assembly L1320263 is attached to the instrument and completely encloses the EXC and COL couplers at the rear of the instrument The EXC and COL screw in caps are fully screwed into their ports before the fiber optic probe cover assembly is secured in place A Class 1 laser product is safe under all reasonably foreseeable conditions of use and may be u...

Page 47: ... on the Instrument on page 48 Wait a few moments for your instrument to initialize and then connect the instrument USB cable to the PC The instrument then needs to be added to the software See Installing a Raman Instrument in the Spectrum Administrator s Guide L1050095 You can add multiple FT IR spectrometers to Spectrum on the same PC as a Raman instrument However you can only add one Raman instr...

Page 48: ... V PSU L1323525 from the DD POWER socket on the rear panel to a mains power socket 3 If your instrument is fitted with a deep depletion CCD connect the Tygon tubing between the water recirculator L1323010 and the WATER IN OUT connectors on the rear panel fill the storage tank of the cooler with deionized water and then plug in and switch on the recirculating cooler For more information about the r...

Page 49: ...Installing Your RamanStation 400 Series 49 5 If appropriate connect the motorized XYZ stage controller box to a mains power socket and switch it on The green LED on the controller box will light up ...

Page 50: ...Using the Joystick or using the Stage Position controls on the Setup Instrument XYZ Stage tab move the stage to its lowest position in the Z axis and then roughly to the center of possible travel in the X and Y axes until the hole for the packing bolt is clearly visible see Figure 16 on page 43 The front and right sides of the stage will be aligned 3 Place the packing bolt in the hole in the cente...

Page 51: ...de is positioned over the edge of your workbench WARNING While the instrument is not stood flat on the bench you must keep it supported at all times 3 Locate and remove the two screws securing the holster bracket to the base of the instrument located behind the front foot Figure 23 Base of the RamanStation 400 Series instrument 4 Return the instrument to its correct position on your workbench Scre...

Page 52: ...52 RamanStation 400 Series Getting Started Guide ...

Page 53: ...Introduction to the RamanStation 400 Series ...

Page 54: ...ptical path of the instrument only that the laser power is on Active This LED indicates the status of the laser shutters If the LED is illuminated then the shutters are activated open and laser radiation present throughout the optical path of the instrument NOTE If you open the sample compartment lid when the Active LED is illuminated the safety interlocks will close the shutters The control butto...

Page 55: ... 400 Series 55 Sample Compartment To open the sample compartment lift the cover using the handle at the front of the RamanStation 400 Series as shown in Figure 25 Figure 25 Opening the sample compartment of the RamanStation 400 Series ...

Page 56: ...and joystick L1865723 are powered by the stage controller box L1860289 Figure 26 Stage controller and joystick NOTE When the joystick is used to move the stage care should be taken that excessive movement does not bring the lens snout into forceful contact with the sample or stage as this may cause damage to both ...

Page 57: ...ttes sample tubes and capillary tubes Figure 28 Versatile Sample Holder NOTE When the joystick is used to move the stage care should be taken that excessive movement does not bring the camera into forceful contact with the sample as this may cause damage to both Particular care should be taken when using the Versatile Sample Holder The Raman Polarization Accessory L1320284 that can be fitted to th...

Page 58: ... 59 for more details which enables the remote measurement of samples The RamanStation 400F instrument can also be used in conjunction with the RamanMicro 300 Raman microscope The RamanMicro 300 Raman microscope system is designed to produce high quality Raman data from either physically small samples such as fibers and single crystals or from small areas of larger samples such as contamination on ...

Page 59: ...Fiber Optic Probe ...

Page 60: ...or details of how to use a triggered fiber optic probe see the Raman Triggered Fiber Optic Probe leaflet L1321940 Please read and understand the following general safety precautions before proceeding If you do not understand any of the details regarding general safety precautions please contact PerkinElmer before proceeding Your instrument should have been installed in accordance with Installing Y...

Page 61: ...ication However it should be noted that compatibility of the working environment with the probe should be verified with PerkinElmer prior to use The probe body contains the filtering optics and is interfaced with two fiber optic cables an excitation fiber and collection fiber Care should be taken when handling the probe body so that the optical window is not soiled or damaged NOTE If you use the p...

Page 62: ... the main power cable and if appropriate the stage controller power cable 3 Carefully move the instrument to permit easier access to the rear panel 4 Remove the fiber port cover assembly if fitted see Attaching the Fiber Port Cover Assembly on page 46 5 Remove the two retained screw in caps from the coupler ports at the bottom left of the panel 6 Connect the excitation cable of the FOP to the coup...

Page 63: ...r panel 4 If you have a standard probe remove the DIN connector from the socket marked LED FIBER 1 OR If you have a triggered probe disconnect the connect the USB extender cable from the USB port on your PC 5 Carefully remove the retained ends of the FOP by unscrewing and pulling the fiber optic cables away from the EXC and COL couplers perpendicularly Be extremely careful when removing the FOP as...

Page 64: ...Station 400F and a fiber optic probe or RamanMicro 300 Accessory is connected ensure that the probe or microscope objectives are covered or if fitted that the microscope enclosure is closed and that the microscope illumination is off and the switch is set to Dark Field DF when using the sample compartment to prevent contamination Sampling Liquids Liquid samples can be analyzed by immersing the FOP...

Page 65: ...orescence is a problem and it is not possible to use quartz containers then it is possible to acquire a spectrum of the container and spectrally subtract this from any spectra containing this fluorescent signature Sampling Slurries Highly absorbing species Please refer to Sampling Liquids For sample immersion care taken in focusing the probe within the sleeve can result in a dramatic improvement i...

Page 66: ...66 RamanStation 400 Series Getting Started Guide ...

Page 67: ...Using the RamanStation 400 ...

Page 68: ...tarting point to develop your own SOPs that better suit your specific mode of use This chapter also acts as a learn by doing section allowing you to explore the functionality of the software and the spectrometer The General SOPs demonstrate how to setup your instrument and software For SOPs that demonstrate how to collect data using a fiber optic probe please refer to Fiber Optic Probe SOPs For st...

Page 69: ...on 5 To turn on the spectrometer press the PSU power switch ON Figure 32 ON Ο OFF Figure 32 The PSU power switch 6 Check that the Power LED on the instrument keypad is On The Laser LED and the Active LED should be off If the Power LED is not on please turn the instrument off at the PSU and check all of the connections any power socket switches and then switch the PSU on again 7 If appropriate conn...

Page 70: ...ooled Messages will be displayed in the Status bar at the bottom of the workspace and on the Scan icons on the Measurement bar If you have a motorized XYZ stage and have just switched on the stage controller box you will be required to reset the stage before you can use the stage controls SOP 2 Turning the Instrument Off 1 Shut down Spectrum software by selecting Exit from the File menu to return ...

Page 71: ...tware SOP 3 Installing and Changing Sampling Holders This SOP describes changing sample holders on the motorized XYZ stage The standard XYZ sample accessory holds a variety of sample holders This procedure outlines installing a 96 multi well plate and then changing to the sample bottle on the Versatile Sample Holder NOTE For information on the use of a polarization accessory see the Raman Polariza...

Page 72: ...mple accessory stage then drives to a preset position 10 Install the 96 well plate Slide the plate into the motorized stage being careful to avoid the snout The A1 position for all multi well plates is the left rear corner as you look at the sample accessory stage 11 Close the sample compartment lid 12 Click OK The stage will then move to the A1 position The instrument can now be used to position ...

Page 73: ...bottle mount when the versatile sample holder is installed Figure 33 Unscrew the lens snout and rotate 5 Select the Setup Instrument Basic tab 6 In the Accessory section select Horizontal as the Snout Orientation The Sample Holder drop down list will update to include sample holders suitable for this snout orientation the stage will not move 7 Select Versatile 7 mm Sample Bottle from the Sample Ho...

Page 74: ...an Instrument from the Setup menu The Setup Instrument tabs are displayed in the dialog pane 5 If required select the appropriate Accessory from the drop down list on the Setup Instrument Basic tab The accessories available to you will depend on your instrument configuration Depending on the Accessory you have selected further options may become available See SOP 3 Installing and Changing Sampling...

Page 75: ...ing patterns are co added to produce one spectrum For more information see the Spectrum on screen Help 14 If you have a Sample Area XYZ Motorized stage select any Focus options You can select to perform a Raman AutoFocus or Video AutoFocus before each sample measurement 15 When you have finished defining your experimental parameters position and optimize conditions for your sample as described in ...

Page 76: ...nitor to help position your sample for optimum spectral collection efficiency Sample positioning and alignment in Monitor mode allows you to position and focus on a specific area and to collect spectral data from that location It can also be used to enhance the signal to noise ratio of spectral data The data displayed on screen during monitoring cannot be saved To save data you must collect spectr...

Page 77: ...form an automatic video focus or Raman AutoFocus to focus using the strongest Raman signal Refer to the Spectrum on screen help for more information 7 Click The RamanStation 400 will start collecting data in the Live display 8 Adjust the Laser power in the Live display to an appropriate value for your sample 9 Adjust the Exposure time s This will automatically acquire another background The sample...

Page 78: ...enu in Spectrum The Setup Instrument tabs are displayed in the Dialog pane 3 Select the Setup Instrument Basic tab 4 Select the Fiber Optic Probe you want to use from the Accessory drop down list The following warning message is displayed 5 Ensure that laser safety precautions are being observed and then click Continue If you have any doubts about the required safety precautions click Cancel Laser...

Page 79: ...b will be displayed and real time spectral acquisition will commence 3 Adjust the Laser power on the Live display to an appropriate value for your sample 4 Adjust the Exposure time s This will automatically acquire another background The sample may now be positioned and aligned correctly for optimized data collection by adjusting the distance between the Fiber Optic Probe and the sample 5 If requi...

Page 80: ... a necessary part of the calibration routines so it is always performed The spectrometer has no moving parts in its detection system therefore frequent calibration is not necessary We recommend that you calibrate the instrument in accordance with the following SOP SOP 8 Calibration Performance Verification We recommend that wavelength calibration verification is performed on a regular basis to ens...

Page 81: ...ation verification to ensure that instrument has been brought back within specification If the instrument fails the wavelength calibration verification again please contact PerkinElmer SOP 9 Wavelength Calibration Verification 1 Ensure the RamanStation 400 Series is switched on If not follow SOP 1 2 Select Raman Instrument from the Setup menu The Setup Instrument tabs are displayed 3 Select the Se...

Page 82: ... Position controls on the Setup Sample Area XYZ Stage tab Alternatively click Raman AutoFocus to focus using the strongest Raman signal The Focus Limits should be set to Full Range 10 Select Toolbox on the Setup Instrument Advanced tab The Adjustments Toolbox dialog is displayed 11 Select The Wavelength Calibration Verification dialog is displayed 12 Ensure that you have followed the instructions ...

Page 83: ...ssory can be purchased separately 3 Ensure that the RamanStation 400 Series sample compartment is closed and if you have a RamanStation 400F ensure that any external accessory is capped or covered to prevent stray light interfering with the measurement If you have a RamanMicro 300 Accessory connected to your RamanStation 400F ensure that the microscope illumination is switched off 4 Select the Set...

Page 84: ...o the sample stage following the instructions on screen if you have a motorized stage 7 Ensure that the RamanStation 400 Series sample compartment is closed and if you have a RamanStation 400F ensure that any external accessory is capped or covered to prevent stray light interfering with the measurement If you have a RamanMicro 300 Accessory connected to your RamanStation 400F ensure that the micr...

Page 85: ...is to be acquired Otherwise any data should be used for qualitative analyses only The NIST intensity correction is valid in the recommended operating temperature range 20 C 25 C If you move the instrument to an environment outside this temperature range that is 15 C 20 C or 25 C 30 C we recommend that you perform a NIST intensity correction at this new temperature Contact your PerkinElmer Raman Bu...

Page 86: ...es sample compartment is closed and if you have a RamanStation 400F ensure that any external accessory is capped or covered to prevent stray light interfering with the measurement If you have a RamanMicro 300 Accessory connected to your RamanStation 400F ensure that the microscope illumination is switched off 8 Select the Setup Instrument Advanced tab and ensure that the laser power is set to 100 ...

Page 87: ... on armed and the laser power is 100 Summary The only calibration procedure that needs to be performed on a regular basis is the wavelength calibration verification procedure The wavelength and laser frequency calibrations should only be performed if the instrument fails the wavelength calibration verification procedure We recommend that the intensity calibration is performed on a yearly basis unl...

Page 88: ...1033 NIR rectangular cell with PTFE stopper Quartz SUPRASIL 300 light path 10 mm L1320284 Raman Polarization Accessory L1320280 Powder Holder Kit for Motorized Stage Contains powder holder baseplate powder cup loading base and tray powder holder cups 10 cm micro spatula brush and tweezers L1320278 Tablet Holder Kit for Motorized Stage Contains tablet holder baseplate sample discs tablet holder cup...

Page 89: ...306 Disposable micropipettes 2 µl 100 pack Safety and Calibration Part Number Description L1320262 Raman Laser Safety Kit 785 nm Contains external interlock cable laser glasses laser detection card and door warning label L1320237 Raman Calibration Kit 785 nm Contains neon lamp and fiber optic connector for wavelength calibration calibration plate containing polystyrene for wavelength calibration v...

Page 90: ...3 Purge Jacket for Raman probe max required to enable max probe operation at temperatures between 200 C and 500 C L1320030 Raman triggered probe 7 5 mm working distance maximum working temperature 80 C 5 m cabling L1320031 Raman triggered probe 7 5 mm working distance maximum working temperature 80 C 20 m cabling L1320070 Short immersion sleeve for TFOP for use with L1320030 and L1320031 L1320324 ...

Page 91: ...Maintenance and Troubleshooting ...

Page 92: ... immediately If anything that is spilled enters the main body of the spectrometer make the instrument inoperative and then contact a PerkinElmer Service Engineer WARNING Do not directly clean electrical contacts WARNING To avoid any electrical shock hazards do not allow liquids to run down into the external PSU Also do not allow liquids to run down into the sample compartment of the spectrometer t...

Page 93: ...ing maintenance or servicing of the FOP The immersion sleeve if purchased is manufactured with 316 stainless steel or Hastelloy C and contains either a quartz or sapphire optical window that is sealed The quartz window can be removed for cleaning by unscrewing the end of the immersion tube The sapphire window cannot be removed as it is welded into place If removing the quartz window do not lose th...

Page 94: ...rdous Class 3B laser radiation to be emitted and will invalidate the instrument warranty 1 Switch off the stage controller disconnect it from the power supply and remove the mains cord 2 Insert a screwdriver into the slot at the side of the fuse drawer and pull out and flip to one side over the mains inlet The fuse may now be removed 3 Fit the replacement fuse into the fuse drawer Make sure that y...

Page 95: ...mple compartment lid and any other remotely interlocked doors are closed properly Solution 2 If you have a fiber optic probe fitted to your system ensure your external interlock is wired up correctly and that any interlocked room entrances or sampling enclosures are closed If you do not have a fiber optic probe ensure that the Magnetic actuator a red block positioned on the inside of the sample co...

Page 96: ...lens snout in the Horizontal orientation Solution 3 If the above solutions do not solve your problem you should contact your PerkinElmer Service Representative Problem The message Video Image not available is displayed on the Live Video image on the Setup Sample Area or Setup Sample Area XYZ Stage tab Description This means that the software searched for the alignment camera on the system and coul...

Page 97: ...ntamination The customer s responsible body is required to follow the Equipment Decontamination Procedure and complete the Certificate of Decontamination These documents are available on the PerkinElmer public website http las perkinelmer com OneSource decontamination htm Alternatively if you do not have access to the internet contact Customer Care Customer Care USA 1 800 762 4000 inside the USA 8...

Page 98: ...t is the most effective means of protecting the customer s environment The requirements for waste collection reuse recycling and recovery programs are set by the regulatory authority in your location Contact your local responsible person such as your laboratory manager or authorized representative for information regarding applicable disposal regulations See the PerkinElmer web address below for i...

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