PBI Barocycler 2320EXT Operator'S Manual Download Page 1

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Barocycler 2320EXT

Pressure Cycling Technology (PCT)

Sample Preparation System

Operator’s Manual

Operator’s  Manual Version: 2.4, June. 2017

Pressure BioSciences, Inc.
14 Norfolk Ave.
South Easton, MA 02375
508-230-1828

Summary of Contents for Barocycler 2320EXT

Page 1: ...320EXT Pressure Cycling Technology PCT Sample Preparation System Operator s Manual Operator s Manual Version 2 4 June 2017 Pressure BioSciences Inc 14 Norfolk Ave South Easton MA 02375 508 230 1828 ww...

Page 2: ...ion owned by Pressure BioSciences Inc that covers the Pressure Cycling Technology Sample Preparation System any composition reagent or kit or any process Specifically but without limitation no right i...

Page 3: ...zardous currents and voltages may be present inside the unit The pressure vessel hardware may be hot when operating at high temperatures Appropriate personal protection insulating gloves or glove line...

Page 4: ...13 3 2 Advanced Features of the 2320EXT Software 18 3 3 User Management GLP GMP and Electronic Records Compliance 21 4 Maintenance 23 4 1 General Maintenance 23 4 2 Confirm Quality of Compressed Air...

Page 5: ...ct the power interface cables and operate the instrument This symbol on the instrument states that high voltage or high current may be present when panels covers are removed Any adjustment maintenance...

Page 6: ...ctions and diagnostic error messages see the troubleshooting guide Section 5 The chamber closure features an internal check valve that is used to allow air or residual pressure to be purged from the c...

Page 7: ...onnected to a water bath is achieved via the water bath controller and cannot be set via the 2320EXT software When connected to a circulating water bath the 2320EXT WJ chamber can be heated up to 95 C...

Page 8: ...edded NOTE Automatic Logon for Default User is disabled on 2320EXT Software v1 3 5 1 and higher Administrator must set up user community Default user cannot use the system Non default users must have...

Page 9: ...n temperature can be set in the range between room temperature and 95 C Note that the 2320 EXT is not equipped with a built in chiller Setting temperature to below ambient will not result in chamber c...

Page 10: ...r in order to distribute force evenly on the connector screw Avoid using fewer than 4 tubes per cartridge as this may result in bending of the connector screw Blanks can be re used multiple times Asse...

Page 11: ...t until its full stop NOTE The instrument will not run while the cover is open If the Cover not in place pop up window is on the screen check the position of the cover and then close this warning wind...

Page 12: ...gain The run will continue where it left off Note that if the run was paused during the high pressure step the chamber will remain pressurized and therefore locked The Pause feature should not be used...

Page 13: ...cuation of the facility To stop operation of the system and shut it down Press the STOP button on the software main screen Wait until the system is depressurized and STOP button is greyed out Press Wi...

Page 14: ...le feature which can be toggled ON or OFF Note Autoscale feature must be turned off for manual scaling of that axis to work Start and Pause Button Pressing the Start button starts the pressure run Dur...

Page 15: ...en pressurizations the chamber pressure will drop to 0 kpsi i e ambient pressure Temperature set the desired temperature for the pressure run Allow unit at least 30 minutes to reach the desired temper...

Page 16: ...o a hh mm ss format i e 312 00 05 12 0 Duplicate time values are not allowed in the Ramp mode program The time values entered are additive The software will warn you if you have entered an invalid ram...

Page 17: ...only on units equipped with electrical heating option Offset Set the pressure level around which the waveform will oscillate Amplitude Set the total amplitude of the pressure waveform For example if...

Page 18: ...d on the Services tab this feature facilitated communication between the software and the instrument control circuits The use of this feature is reserved for the PBI service personnel Additional Admin...

Page 19: ...fault password is password See additional information on user management features in Section 3 3 below o Modify Users allows creating or modifying new users and passwords with two levels of privileges...

Page 20: ...T is equipped with a built in thermocouple and is configured to provide temperature logging within 0 5 C Fine tuning of the temperature reading may require changing the calibration of the thermocouple...

Page 21: ...vice screen System log runs automatically in a privileged mode none of the users can stop logging or alter the type of information that is being logged o Raw Data Logging and Storage Options If the op...

Page 22: ...ctivity to an External LIMS System System factory configuration includes an option called LIMS When enabled this option forces user to enter a 12 character alphanumeric run ID before a new run can be...

Page 23: ...Greater usage may require more frequent maintenance Table 2 Maintenance Schedule for optionally provided air compressor Task Weekly Monthly Annually Check oil levels in all heads and refill if necess...

Page 24: ...indows Embedded Standard Event Logs for critical errors X Check and correct MS Windows Date Time settings X Run MS Windows Update routine if system is not connected to the network or not configured to...

Page 25: ...e Section 4 4 Replacing the rubber O ring Step 1 Pull the used rubber O ring orange arrow color of the O ring may vary up and over the metal retaining ring green arrow Step 2 Inspect the area under th...

Page 26: ...e tubing while the chamber is trying to reach pressure replace closure O ring see instructions above If water continues to flow from this tubing there is an issue with the Closure Check Valve Clean ch...

Page 27: ...ebiosciences com Click on Contact Us at the top of the page Please provide your name contact information and question s To Order more MicroTubes MicroCaps MicroPestles or other Barocycler accessories...

Page 28: ...the door by sliding it all the way toward the front of the instrument Note the door error message is not displayed if the door was open at the time software was started Opening the door after softwar...

Page 29: ...control system If the control software malfunctions or freezes for any reason the confirmation signal will be lost and the heater will be disabled Contact PBI Customer support The unit fails to start...

Page 30: ...d water discharge through the attached valve would indicate that instrument service is required Contact PBI support if this happens The red tubing is connected to the intensifier seal Leakage of fluid...

Page 31: ...required at the rear and sides of the Barocycler Electrical Requirements North America Japan configuration 100 120 VAC 50 60 Hz Electric power consumption 100 watts Europe configuration 200 230 VAC 5...

Page 32: ...The benefit to the customer is a faster time to repair interval and possibly a lower cost repair as well The customer must provide access for the repair of the instrument during normal working hours...