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LE 5002 NON

INVASIVE BLOOD

PRESSURE METER

V25/05/12

USER MANUAL

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Summary of Contents for LE 5002 NON

Page 1: ...LE 5002 NON INVASIVE BLOOD PRESSURE METER V25 05 12 USER MANUAL ...

Page 2: ...sunlight B Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed WARNING For electrical safety reasons you only ca...

Page 3: ...VER pour water or liquids on the equipment Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use The user is only authorised to replace fuses with the specified type when necessary Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT OR VOLTAGE SETTING CHANGE In case of an over volt...

Page 4: ...ses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert the fuse holder again positioning it according to the voltage in the AC net 115V POSITON 230V POSITION Figure 5 Fuse holder position 6 If the fuses blow again unplug the equipment and contact technical service WARNING For electrical safety reasons never open the equipment The...

Page 5: ...EL ROW 12 6 EQUIPMENT CONNECTION 13 6 1 LE 5002 13 6 2 WORKING WITH HEATER AND SCANNER 14 6 3 WORKING WITH NIBP CHART USB 16 7 FACTORS TO TAKE INTO ACCOUNT 17 7 1 ON THE PLACEMENT OF THE PRESSURE CUFF AND PULSE TRANSDUCER 17 7 2 ON THE PROPER TREATMENT OF THE ANIMALS AND THEIR ENVIRONMENT 18 7 3 WHY MUST THE ANIMAL BE VASODILATED 18 7 4 RECOMMENDED METHODOLOGY 19 7 5 ANAESTHETISING THE ANIMALS 20 ...

Page 6: ...OPERATION 28 12 1 PUMP CALIBRATION 28 12 2 SIMULATING SYSTOLIC PRESSURE 29 12 3 TROUBLESHOOTING 29 12 4 TRANSDUCER 30 12 5 PROCEDURE TO CLEAN PULSE TRANSDUCERS 30 12 6 REPLACING THE CUFF MEMBRANE 31 12 7 CUFF AND TRANSDUCER MAINTENANCE 32 13 TECHNICAL SPECIFICATIONS 33 ...

Page 7: ...is displayed continuously and then stored along with the corresponding systolic diastolic and mean pressure values A measurement is comprised of the blood pulse heart rate and systolic and diastolic pressure values All measurements up to 6000 data are memorised internally they are not deleted when the unit is switched off so they can be retrieved and displayed in the LE 5002 or sent to a PC using ...

Page 8: ...r used to adjust signal from the transducer DISPLAY 4 row 20 column display It is used to display the result of measurements and several messages depending on the mode the instrument is in SEND Button used to send data to the computer using RS 232 cable and SEDACOM software It activates one of 2 options o SEND ACTIVE TRIAL Sends only the trial shown in the display o SEND ALL MEMORY Sends all data ...

Page 9: ... technical service mode If there is no signal or the signal level is lower than the necessary level INSUFICIENT LEVEL or if there is too much signal PULSE LEVEL HIGH the start button will not act If the pulse level is correct PULSE LEVEL READY the start button will begin taking measurements STOP Button used to cancel a currently running measurement It is also used to test the pump in technical ser...

Page 10: ...el of signal It can be connected to a data recorder system UP DOWN Selector of mode of systolic pressure detection Results obtained in both modes are different o UP Systolic pressure is detected when pump inflates This mode is for DOG o DOWN Systolic pressure is detected when pump deflates through the exhaust valve This mode is for RAT and MOUSE DOG RAT MOUSE 3 position filter It selects the windo...

Page 11: ...essure DIA Diastolic pressure MED Mean pressure calculated with the formula 33 0 DP SP DP MP 5 3 2 MESSAGE ROW This line shows several messages depending on the instrument s current status They are listed below 5 3 2 1 Auto Calibration The system takes a few seconds to auto calibrate after the instrument is turned on 5 3 2 2 Pulse level Ready This message is displayed when the pulse level received...

Page 12: ...T and while the measurement is being carried out the pump is working and pressure increases once the pulses have disappeared pressure is slowly released through an exhaust valve until the signal recovers its original level 5 3 2 6 Deflating Once measurement has been completed the air in the pump must be emptied to prepare the instrument for a new measurement This is done with an electro valve This...

Page 13: ...re 10 Equipment connections The necessary connections are detailed in the next table FROM TO CABLE 1 LE 5002 Transducer Transducer DIN 6 cable 2 LE 5002 Cuff2 Cuff Silicone pipe 3 LE 5002 RS 232 Computer serial port RS 232 cable 4 LE 5002 Remote Foot switch 6 35mm mono Jack 2 Pneumatic connection ...

Page 14: ...LE 5002 Non Invasive Blood Pressure Meter USER MANUAL 14 6 2 WORKING WITH HEATER AND SCANNER Figure 11 Connection between LE 5002 and LE 5650 ...

Page 15: ... 5002 RS 232 Computer serial port RS 232 cable 5 LE 5650 Transducer 1 Transducer 1 DIN 6 cable 6 LE 5650 Transducer 2 Transducer 2 DIN 6 cable 7 LE 5650 Transducer 3 Transducer 3 DIN 6 cable 8 LE 5650 Transducer 4 Transducer 4 DIN 6 cable 9 LE 5650 Transducer 5 Transducer 5 DIN 6 cable 10 LE 5650 Transducer 6 Transducer 6 DIN 6 cable 11 LE 5650 Cuff 13 Cuff 1 Silicone pipe 12 LE 5650 Cuff 23 Cuff ...

Page 16: ...ucer DIN 6 cable 2 LE 5002 Cuff4 Cuff Silicone pipe 3 LE 5002 Remote Foot switch 6 35mm mono Jack 4 NIBP Chart Pressure LE 5002 Cuff Pressure BNC cable 5 NIBP Chart Pulses LE 5002 Pulses BNC cable 6 NIBP Chart USB Computer USB port USB cable In order to work with the NIBP Chart USB the NIBP Chart software must be installed on the computer Read the user manual for further information on this softwa...

Page 17: ...hould always be done with the animal properly prepared See Vasodilatation This manual describes some tips that are for guidance only The animal s tail should be kept clean This will improve contact between skin cuff and pulse transducer The cuff pulse transducer unit can be used either together or separately There is no need to press the tail excessively with the transducer The pressure applied by...

Page 18: ...unstressed condition when its pressure is taken the pressure values obtained will not be those expected It is not like taking blood pressure in human beings The precautions to be taken to diagnose abnormal stress should be obvious A stressed rodent may transmit the muscular tremors produced by anxious breathing to its tail These tremors will mask the signal of the heart beat to be captured by the ...

Page 19: ...ven as guidance only RATS between 29ºC and 32ºC MICE between 30ºC and 34ºC Higher values may jeopardise accomplishment of the objective Experience has shown us that the best results are obtained by drawing out heating time rather than by increasing the temperature The following description outlines a typical protocol that should provide satisfactory results in the measuring processes Naturally it ...

Page 20: ... also be considered This reduction in body temperature triggers a fall in the flow of blood circulating through the vessels of the tail It may therefore be necessary to substantially increase the temperature which in this case may be done locally heating the tail only Disproportionate heating temperatures do not make measurement easier Generally there is a temperature value determined by experimen...

Page 21: ...ate Point 1 is the systolic pressure taken in UP mode 2 The pressure value in the sleeve at the time when the blood pulses reappear point 2 is equivalent to the systolic pressure in DOWN mode 3 The pressure in the sleeve continues to fall until it reaches the value of diastolic pressure which is the pressure value corresponding to the instant when the pulse wave recovers its initial value point 3 ...

Page 22: ... Unsuitable pulse Figure 15 Correct pulses The pulse wave PULSE WAVE plug outlet and the pressure variation PRESSURE CUFF of the cuff can be reproduced on paper with recorders oscilloscopes or a data capture system such as PowerLab BioPac or to the NIBP Chart software ...

Page 23: ...e TRANSDUCER input WARNING The slot on the male connector must be aligned with the input slot always above it To secure the Pulse transducer as an additional safety measure the front cylinder of the connector should be screwed in to the right to secure its position To disconnect the transducer first unscrew said front cylinder to the left WARNING Never unscrew the main connector cylinder body Figu...

Page 24: ...l is achieved or readjust the GAIN controller Take special care with the stability of the pulse signal level as the accuracy of the diastolic pressure measurement is strongly dependent on it Animal movements are also detected by the pulse transducer and would mask the blood pressure signal If the maximum gain is reached without obtaining an adequate and stable pulse level check the transducer fitt...

Page 25: ...s reached and the current diastolic pressure value will be kept under the DIA label on the digital display The mean blood pressure is then calculated as stated in the Introduction to this manual and its value shown under the MED label on the digital display If a new animal is going to be studied connect its tail cuff and transducer to the instrument or if a scanner is available select the animal n...

Page 26: ...llow next instructions Please refer to the SEDACOM User s Manuel for the instruction about how to install and use the software with the present device A serial port RS232 communication cable provided with the present device is needed for the connection of the present devices to the computer in which the SEDACOM software is installed Please refer to the present User s Manual for the instruction abo...

Page 27: ...arrows Clear active trial Clears the measurement currently shown on the display Users can navigate through the stored data using up down arrows before entering in the Clear menu Clear all memory Erases all the stored data and resets the TRI counter to 1 To confirm the option you have selected press the CLEAR button again WARNING Be careful when erasing data Once it is erased it cannot be recovered...

Page 28: ...ed pressure value has been reached e g about 200 mmHg The pressure value will be displayed briefly on the digital display as it will immediately start to fall when the exhaust valve is opened To stabilise the reading remove the cover of the Unit and cover the air output hole with your finger The air output hole is located on the bottom of the pressure pump see next figure Figure 20 Pump valve If t...

Page 29: ...nually press the transducer until the READY level is achieved and then press START Wait until the pressure increases to a given value and then interrupt the vibrations pulses and check that the pressure falls again 12 3 TROUBLESHOOTING SYMPTOM SOLUTION The pulse transducer gives off signals sometimes erratic regular without the animal connected the BEAT light flashes erratically The transducer is ...

Page 30: ...EDURE TO CLEAN PULSE TRANSDUCERS To clean the transducers we will use alcohol talcum powder and a cotton swab Please follow these instructions to avoid damaging the transducer during the cleaning procedure Figure 21 Alcohol and talcum powder 1 Wet the cotton swab with alcohol Figure 22 Wet the cotton with alcohol 2 Put the cotton swab in parallel position to the sensor and brush softly by turning ...

Page 31: ...system When such wear has occurred they should be replaced Naturally the service life of the membranes will depend on how well serviced they are and the number of operations carried out Generally they should be changed when the first signs of loss of flexibility or drying are observed The membrane must be an original PANLAB component The use of parts made by other manufacturers is not recommended ...

Page 32: ...circumference of the cuff 5 Fit the O ring to the cuff to secure the end of the membrane 6 Repeat point 4 with the other end making sure that the membrane walls are not taut 7 Fit the other O ring and close the ends with the rings The procedure for replacing the membrane of cuffs for mice is very straightforward as in these cases the membrane must be taut and well adjusted 12 7 CUFF AND TRANSDUCER...

Page 33: ...PM From 360 BPM to 1020 BPM 2 BPM o 0 5 of reading PRESSURE SENSOR Resolution Range Maximum over pressure Total error linearity sensitivity and hysteresis at 25ºC Precision 1mmHg 50 a 300 mmHg 400 a 4000 mmHg 2 reading or 1mmHg the highest 3 reading or 1mmHg the highest ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity Storage temperature 10 C to 40 C 0 to 85 RH non conden...

Page 34: ...s D C 73 23 CEE D C 89 336 CEE D C 93 68 CEE D C 2002 96 CE Directiva de baja tensión Low Voltage Basse tension Directiva de compatibilidad electromagnética y su modificación D C 92 31 CEE EMC Modificaciones y marcado CE Residuos de aparatos eléctricos y electrónicos Para su evaluación se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were appl...

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