Multi-Well Plate Vacuum Manifold
Description
The Multi-well Plate vacuum manifold is an anodized aluminum manifold that has
been designed and optimized for the vacuum filtration of the AcroPrep
™
and
AcroWell
™
lines of multi-well filter plates. The vacuum manifold comes complete
with the necessary O-ring and gasket. The control block includes the vacuum
pressure gauge, vacuum metering valve, vacuum release valve and the 1/4 inch
hose barb for vacuum line attachment. Included with the vacuum manifold unit is a
Delrin spacer block designed to accommodate standard 350 µL receiver plates.
The spacer block has been optimized to reduce the space between the receiver
plate and the filter plate during vacuum filtration. A spacer block that has been
optimized to work with our 1 mL plate is available separately. An accessory kit that
contains an additional O-ring, gasket and allen wrench is also included with the
vacuum manifold unit.
Ordering Information
Prod. No. Description
Packaging
5017
Multi-well Plate Vacuum Manifold
1/pkg
5014
1 mL Receiver Plate Spacer Block
1/pkg
5015
350 µL Receiver Plate Spacer Block
1/pkg
5016
Replacement Accessory Kit (Includes O-Ring,
1/pkg
gasket and allen wrench)
Note:
the 350 µL Receiver Plate Spacer Block can be used to accomodate 384
well receiver plates as well.
Specifications
Materials of Construction
Vacuum Manifold: Anodized aluminum
Gasket: EDPM (Ethylene propylene)
O-ring: Silicone
Spacer blocks: Delrin
Dimensions
Length: 17.48 cm (6.88 in)
Width: 12.37 cm (4.87 in)
Height: 8.05 cm (3.17 in)
Weight: 6.27 lbs
Maximum Operating Vacuum
71.12 cm Hg (28 in Hg).
Note:
The multi-well plate vacuum manifold can be used with multi-well filter plates
that meet the specifications set forth by the Society for Biomolecular Screening
(SBS).
Instructions for Use
To set the vacuum manifold to a desired negative pressure setting prior to filtration,
follow the steps listed below.
1
1. Switch the on/off valve to the off position (See Figure 3).
1
2. Attach the vacuum line to the 1/4 inch hose barb.
1
3. Place a receiver plate on top of the vacuum manifold.
1
4. Turn on the vacuum pressure. You may need to press lightly on the receiver
plate to engage the vacuum seal.
1
5. Adjust the negative pressure to the desired setting by adjusting the metering
valve.
1
6. Turn off the vacuum pressure and remove the receiver plate. The vacuum
manifold is now set at the desired setting.
To filter the AcroPrep or AcroWell filter plates follow the steps listed below.
1
1. If collecting the filtrate, remove the top chamber of the vacuum manifold. The
indentations on the sides of the manifold allow for the top portion of the
manifold to be easily removed. If collecting the retentate, skip to step 3.
1
2. Place the appropriate spacer block into the lower chamber of the vacuum
manifold. The vacuum manifold can be used with any size receiver plate.
350 µL receiver plates: To use a 350 µL 96 well or 384 well receiver plate,
place the large spacer block (PN 5015) into the lower chamber of the vacuum
manifold. Place the 350 µL 96 well or 384 well receiver plate on top of the
spacer block.
1 mL receiver plates: To use 1 mL receiver plates, place the small spacer block
(PN 5014) into the lower chamber of the vacuum manifold. Place the 1 mL
receiver plate on top of the spacer block. (You can also use a standard 350 µL
receiver plate in place of the 1 mL spacer block.)
2 mL receiver plates: To use 2 mL receiver plates, place the 2 mL receiver plate
directly into the lower chamber of the vacuum manifold.
Instructions for Use (cont.)
1
3. Before replacing the top chamber of the vacuum manifold, ensure that all
surfaces are free from dirt, debris and any particulate matter that may have
accumulated on the vacuum manifold, O-ring and gasket.
Note:
If the O-ring and gasket are not clean, you will not obtain a proper seal.
1
4. Replace the top chamber of the vacuum manifold.
1
5. Place the filter plate on the gasket located on the top chamber of the vacuum
manifold. Ensure that the gasket is clean.
1
6. Ensure that the on/off switch is in the position (See Figure 3).
1
7. Connect the vacuum line to the 1/4 inch hose barb.
1
8. When ready to evacuate the filter plate, turn the on/off switch on the vacuum
manifold to the on position. You may need to press lightly on the filter plate to
engage the vacuum seal.
1
9. The wells will begin to evacuate/empty once the vacuum has been applied to
the chamber.
10. If you need to adjust the vacuum pressure up or down, adjust by moving the
metering valve located to the right of the vacuum pressure gauge.
11. When all of the wells have completely evacuated, turn the on/off valve to the off
position. To release the residual vacuum pressure that remains in the chamber,
push the release valve located to the left of the vacuum gauge. The release
valve will allow the pressure within the manifold chamber to return to
atmospheric pressure and reduce the potential for cross contamination and
spraying of the filtrate.
Do not release the vacuum by pulling the corner of
the plate as it will degrade the manifold gasket.
You can also tap the top of
the filter plate prior to removing it to release any hanging drops that may be
attached to the outlet tips.
12. Remove the filter plate and place it aside for further processing or dispose of
properly.
13. If collecting the filtrate, remove the top chamber of the vacuum manifold from
the lower portion of the vacuum manifold.
14. Remove the receiver plate from the lower chamber of the vacuum manifold and
utilize the filtrate for further processing.
To replace the O-ring follow the steps below.
1
1. Remove existing O-ring from the bottom of the upper chamber.
1
2. Ensure that the new O-ring and O-ring groove are free from dirt, debris and
particulate matter.
1
3. Place new O-ring into place.
To replace the gasket follow the steps below.
1
1. With the allen wrench provided, remove the 12 screws located on the bottom
portion of the upper chamber.
1
2. Separate the top ring from the bottom section of the upper chamber.
1
3. Remove the old gasket and clean the gasket pocket area of any dirt and
debris.
1
4. Place the new gasket into the gasket pocket. Place the top ring back onto the
bottom section of the upper chamber. Ensure that the alignment posts on the
bottom section fit into the alignment holes in the top ring.
1
5. Invert the upper chamber and replace the 12 screws.
1
6. Lightly tighten the four corner screws with the allen wrench, then tighten all 12
screws. Once this is completed recheck the screws with the allen wrench to
ensure all screws are tightly secured.
1
7. The manifold is now ready for use.
Figure 1
Control block
Vacuum release valve
Vacuum pressure gauge
Vacuum metering valve
On/Off switch
1/4 inch hose barb
350
µ
L spacer block
O-ring
Gasket
Figure 1
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