Mosaic3
50
Version 3.5 rev 8 May 2019
OPERATION
While the focus and size are not critical, they deserve some attention. The first image (left) shows a picture of a poorly
focused spot and the second (right) shows a sharply focused large spot.
Figure 16: (left) Small but poorly focused spot, (right) Larger but sharply focused spot
5.6.2 c
entration
of
the
L
aser
i
LLumination
on
the
dmd
In this discussion, we assume that the eyepieces and the imaging camera are “parcentric” and “parfocal”
i.e. maintain
the subject in centre of field of view and in focus respectively, on changing magnification.
To view the laser spot for size and centration, select the objective to be used with the Mosaic and focus on the
fluorescence sample (provided).
Select the provided Mosaic fluorescence filter cube and imaging camera port for viewing. This will engage the eyepiece
laser interlock and allow the laser to turn on when requested. Configure the Mosaic software or software toolbar installed
in the imaging system (refer to supplied software guide) to illuminate the entire Mosaic field of view and set the laser to a
low power setting. Activate the laser illuminator source and view the spot within the imaging system field of view.
Figure 17: Laser Spot
Determine the centre of the imaging system field of view using your imaging system drawing tools. Use the joystick
“tee” handle (provided) in the Position Socket of the fibre collimator shown in Figure 21 and adjust with pitch and yaw
movements to bring the laser spot to the camera centre.
